公开(公告)号：US12031151B2

公开(公告)日：2024-07-09

申请号：US17578681

申请日：2022-01-19

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Reginald Smith ,  Sarah Nicoletti ,  Victor Shashilov ,  Hongxia Wang ,  Jikang Wu ,  Abdelqader Zamamiri

IPC: C12N5/00 ,  C12N5/071 ,  C12N15/79

CPC classification number: C12N5/0018 ,  C12N5/0682 ,  C12N15/79 ,  C12N2500/32 ,  C12N2500/60

Abstract: Methods of improving recombinant protein titer and cell titer in cell culture using cell culture media having reduced impurities are provided, and well as cell culture media having reduced impurities that can used for the production of a recombinant protein and cells with improved titer. The cell culture media having reduced impurities comprises a HEPES buffer, and the reduced impurities are HEPES related impurities. In certain aspects, methods and media improve protein titer, cell growth, and/or viable cell density.

公开(公告)号：US20220228107A1

公开(公告)日：2022-07-21

申请号：US17578681

申请日：2022-01-19

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Reginald Smith ,  Sarah Nicoletti ,  Victor Shashilov ,  Hongxia Wang ,  Jikang Wu ,  Abdelqader Zamamiri

IPC: C12N5/00 ,  C12N15/79 ,  C12N5/071

Abstract: Methods of improving recombinant protein titer and cell titer in cell culture using cell culture media having reduced impurities are provided, and well as cell culture media having reduced impurities that can used for the production of a recombinant protein and cells with improved titer. The cell culture media having reduced impurities comprises a HEPES buffer, and the reduced impurities are HEPES related impurities. In certain aspects, methods and media improve protein titer, cell growth, and/or viable cell density.

公开(公告)号：US20250059501A1

公开(公告)日：2025-02-20

申请号：US18673522

申请日：2024-05-24

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Reginald Smith ,  Sarah Nicoletti ,  Victor Shashilov ,  Hongxia Wang ,  Jikang Wu ,  Abdelqader Zamamiri

IPC: C12N5/00 ,  C12N5/071 ,  C12N15/79

Abstract: Methods of improving recombinant protein titer and cell titer in cell culture using cell culture media having reduced impurities are provided, and well as cell culture media having reduced impurities that can used for the production of a recombinant protein and cells with improved titer. The cell culture media having reduced impurities comprises a HEPES buffer, and the reduced impurities are HEPES related impurities. In certain aspects, methods and media improve protein titer, cell growth, and/or viable cell density.