公开(公告)号：US20240337664A1

公开(公告)日：2024-10-10

申请号：US18617922

申请日：2024-03-27

申请人： Regeneron Pharmaceuticals, Inc.

发明人： Haibo Qiu ,  Sunnie Kim ,  Xiang Zheng ,  Yimeng Zhao

IPC分类号： G01N33/68 ,  C12N9/10 ,  C12N9/48 ,  C12N9/76 ,  G01N30/02 ,  G01N30/72

CPC分类号： G01N33/6857 ,  C12N9/1044 ,  C12N9/485 ,  C12N9/6427 ,  G01N30/7233 ,  G01N33/6842 ,  G01N33/6848 ,  C12Y304/17002 ,  G01N2030/027

摘要： The present invention generally pertains to methods of characterizing crosslinking sites of a protein of interest. In particular, the present invention pertains to the use of size exclusion chromatography, peptide mapping and subunit analysis to identify and quantify crosslinking sites of a protein of interest and determine a contribution of crosslinking to the formation of high molecular weight species.