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公开(公告)号：US20090263848A1
公开(公告)日：2009-10-22
申请号：US12227186
申请日：2007-04-24
申请人： Stefan Obermann , Reinhard Baumfalk , Oscar-Werner Reif , Florian Wurm , Maria De Jesus , Matthieu Stettler , Martin Jordan
发明人： Stefan Obermann , Reinhard Baumfalk , Oscar-Werner Reif , Florian Wurm , Maria De Jesus , Matthieu Stettler , Martin Jordan
IPC分类号： C12Q1/02
CPC分类号： G01N33/58 , G01N1/30 , G01N15/042 , G01N33/5005 , Y10T436/25375
摘要： The invention relates to a method for determining the viability of cells in cell cultures, said method comprising the steps of: colouring the sample with a dye which can penetrate into individual cells depending on the viability of the latter, determining the proportions of cells which are coloured to differing extents. The invention is distinguished by virtue of the fact that a cell suspension of the coloured cells is centrifuged as a sample in a sample vessel (10) until the solid content present in the sample has settled as a compressed cell cake (13), and, in order to determine the proportions, the volumes of differently coloured sections (131, 132, 133) of the compressed cell cake (13) are determined as a measure of the proportions of cells of different viability.
摘要翻译： 本发明涉及一种用于确定细胞培养物中细胞存活率的方法，所述方法包括以下步骤：根据染色体的活力来染色样品，所述染料可渗透到单个细胞中，确定细胞的比例 彩色到不同的程度。 本发明的区别在于，将着色细胞的细胞悬浮液作为样品在样品容器（10）中离心直到样品中存在的固体含量作为压缩细胞饼（13）沉降， 为了确定比例，压缩细胞饼（13）的不同颜色的部分（131,132,133）的体积被确定为不同存活力的细胞比例的量度。
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公开(公告)号：US08107715B2
公开(公告)日：2012-01-31
申请号：US12227184
申请日：2007-04-24
申请人： Reinhard Baumfalk , Oscar-Werner Reif , Florian Wurm , Maria de Jesus , Martin Jordan , Matthieu Stettler , Stefan Obermann
发明人： Reinhard Baumfalk , Oscar-Werner Reif , Florian Wurm , Maria de Jesus , Martin Jordan , Matthieu Stettler , Stefan Obermann
CPC分类号： G01N21/0303 , G01N2021/0367
摘要： The invention relates to a device for detecting a sample in a longitudinal sample container (10), comprising a sample container holder (30) for holding the sample container in a housing. Said sample container holder comprises a side viewing window in the region of at least one longitudinal side of the sample container, and a front viewing window (35) in the region at least one front side of the sample container. Said device also comprises a first illumination arrangement for illuminating the sample container through the front viewing window, a second illumination arrangement for illuminating the sample container through the side viewing window, and an imaging photodetector for detecting a first image of the sample illuminated by means of the first illumination arrangement, and a second image of the sample illuminated by means of the second illumination arrangement. Said detection takes place through the side viewing window. Said device further comprises a digital data processing unit for overlaying the first and second images and for representing the resulting overlay image on a display device connected to the data processing unit.
摘要翻译： 本发明涉及一种用于检测纵向样品容器（10）中的样品的装置，包括用于将样品容器保持在壳体中的样品容器保持器（30）。 所述样品容器保持器包括在样品容器的至少一个纵向侧区域中的侧视窗和位于样品容器的至少一个正面的区域中的前视窗（35）。 所述装置还包括用于通过前视窗照亮样本容器的第一照明装置，用于通过侧视窗照射样本容器的第二照明装置，以及用于检测通过以下方式照射的样本的第一图像的成像光电检测器： 第一照明装置和通过第二照明装置照亮的样本的第二图像。 所述检测通过侧视窗进行。 所述设备还包括数字数据处理单元，用于覆盖第一和第二图像，并用于在连接到数据处理单元的显示设备上表示所得覆盖图像。
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公开(公告)号：US20090103772A1
公开(公告)日：2009-04-23
申请号：US12227184
申请日：2007-04-24
申请人： Reinhard Baumfalk , Oscar-Werner Reif , Florian Wurm , Maria De Jesus , Martin Jordan , Matthieu Stettler , Stefan Obermann
发明人： Reinhard Baumfalk , Oscar-Werner Reif , Florian Wurm , Maria De Jesus , Martin Jordan , Matthieu Stettler , Stefan Obermann
IPC分类号： G06K9/00
CPC分类号： G01N21/0303 , G01N2021/0367
摘要： The invention relates to a device for detecting a sample in a longitudinal sample container (10), comprising a sample container holder (30) for holding the sample container in a housing. Said sample container holder comprises a side viewing window in the region of at least one longitudinal side of the sample container, and a front viewing window (35) in the region at least one front side of the sample container. Said device also comprises a first illumination arrangement for illuminating the sample container through the front viewing window, a second illumination arrangement for illuminating the sample container through the side viewing window, and an imaging photodetector for detecting a first image of the sample illuminated by means of the first illumination arrangement, and a second image of the sample illuminated by means of the second illumination arrangement. Said detection takes place through the side viewing window. Said device further comprises a digital data processing unit for overlaying the first and second images and for representing the resulting overlay image on a display device connected to the data processing unit.
摘要翻译： 本发明涉及一种用于检测纵向样品容器（10）中的样品的装置，包括用于将样品容器保持在壳体中的样品容器保持器（30）。 所述样品容器保持器包括在样品容器的至少一个纵向侧区域中的侧视窗和位于样品容器的至少一个正面的区域中的前视窗（35）。 所述装置还包括用于通过前视窗照亮样本容器的第一照明装置，用于通过侧视窗照射样本容器的第二照明装置，以及用于检测通过以下方式照射的样本的第一图像的成像光电检测器： 第一照明装置和通过第二照明装置照亮的样本的第二图像。 所述检测通过侧视窗进行。 所述设备还包括数字数据处理单元，用于覆盖第一和第二图像，并用于在连接到数据处理单元的显示设备上表示所得覆盖图像。
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公开(公告)号：US08093015B2
公开(公告)日：2012-01-10
申请号：US12227186
申请日：2007-04-24
申请人： Stefan Obermann , Reinhard Baumfalk , Oscar-Werner Reif , Florian Wurm , Maria de Jesus , Matthieu Stettler , Martin Jordan
发明人： Stefan Obermann , Reinhard Baumfalk , Oscar-Werner Reif , Florian Wurm , Maria de Jesus , Matthieu Stettler , Martin Jordan
CPC分类号： G01N33/58 , G01N1/30 , G01N15/042 , G01N33/5005 , Y10T436/25375
摘要： The invention relates to a method for determining the viability of cells in cell cultures, said method comprising the steps of: coloring the sample with a dye which can penetrate into individual cells depending on the viability of the latter, determining the proportions of cells which are colored to differing extents. The invention is distinguished by virtue of the fact that a cell suspension of the colored cells is centrifuged as a sample in a sample vessel (10) until the solid content present in the sample has settled as a compressed cell cake (13), and, in order to determine the proportions, the volumes of differently colored sections (131, 132, 133) of the compressed cell cake (13) are determined as a measure of the proportions of cells of different viability.
摘要翻译： 本发明涉及一种用于确定细胞培养物中细胞存活率的方法，所述方法包括以下步骤：根据染色体的活力来染色样品，所述染料可以渗透到各个细胞中，确定细胞的比例 彩色到不同的程度。 本发明的区别在于，将着色细胞的细胞悬浮液作为样品在样品容器（10）中离心直到样品中存在的固体含量作为压缩细胞饼（13）沉降， 为了确定比例，压缩细胞饼（13）的不同颜色的部分（131,132,133）的体积被确定为不同存活力的细胞比例的量度。
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公开(公告)号：US20070159920A1
公开(公告)日：2007-07-12
申请号：US11643548
申请日：2006-12-21
IPC分类号： B01F15/04
CPC分类号： B01F7/162 , B01F15/00207 , B01F15/0022 , B01F15/00831 , B01F15/0085 , B01L3/505
摘要： Container and method for the mixing of media, in particular as a disposable container, which has at least one closable opening for the introduction and/or removal of the media and has a first sensor for measurement of at least one parameter of the mixture, in which at least one second sensor for measurement of the same parameter is arranged at a distance from the first sensor.
摘要翻译： 用于混合介质，特别是作为一次性容器的容器和方法，其具有用于引入和/或移除介质的至少一个可闭合的开口，并且具有用于测量混合物的至少一个参数的第一传感器， 其中用于测量相同参数的至少一个第二传感器布置在离第一传感器一定距离处。
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6.发明授权Disposable container having sensor mounts sealed to the container and sensors in the sensor mounts for measuring at least one parameter of media in the container 有权具有密封到容器的传感器安装件和传感器安装件中的传感器的一次性容器，用于测量容器中的介质的至少一个参数公开(公告)号：US08123397B2
公开(公告)日：2012-02-28
申请号：US11643548
申请日：2006-12-21
IPC分类号： B01F15/04
CPC分类号： B01F7/162 , B01F15/00207 , B01F15/0022 , B01F15/00831 , B01F15/0085 , B01L3/505
摘要： A disposable container is provided for mixing media. The container has at least one closable opening for introducing and/or removing media and has sensor mounts at spaced apart locations on the wall of the container. Each sensor mount has a front surface facing into the container and a rear surface sealed against the wall of the container. Sensors are embedded in the respective sensor mounts at positions adjacent the front surfaces. The sensors measure at least one specified parameter of the media at spaced apart locations in the container. The seals between the rear surfaces of the sensor mounts and the wall of the container ensure sterility for the media in the container.
摘要翻译： 提供一次性容器用于混合介质。 容器具有用于引入和/或移除介质的至少一个可关闭的开口，并且在容器的壁上的间隔开的位置处具有传感器安装件。 每个传感器支架具有面向容器的前表面和密封在容器的壁上的后表面。 传感器嵌入相邻的传感器安装座位于与前表面相邻的位置。 传感器在容器中的间隔开的位置测量介质的至少一个指定参数。 传感器支架后表面和容器壁之间的密封确保了容器中介质的无菌性。
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公开(公告)号：US08123395B2
公开(公告)日：2012-02-28
申请号：US12319727
申请日：2009-01-12
IPC分类号： B01F15/04
CPC分类号： B01F7/162 , B01F15/00207 , B01F15/0022 , B01F15/00831 , B01F15/0085 , B01L3/505
摘要： A method is provided for mixing media in a container. The method includes introducing a first media into the container and then introducing a second media into the container while mixing the media to form a mixture. The method continues by measuring the values of two parameters of the mixture at spaced apart locations in the container. The introduction of the second media into the container may be interrupted based on a comparison of the measured values to a preselected intended value.
摘要翻译： 提供了用于将容器中的介质混合的方法。 该方法包括将第一介质引入容器中，然后将第二介质引入容器中同时混合介质以形成混合物。 该方法通过在容器中的间隔开的位置处测量混合物的两个参数的值来继续。 可以基于将测量值与预选的预期值的比较来中断将第二介质引入容器中。
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公开(公告)号：US20090147617A1
公开(公告)日：2009-06-11
申请号：US12319727
申请日：2009-01-12
IPC分类号： G05D11/02
CPC分类号： B01F7/162 , B01F15/00207 , B01F15/0022 , B01F15/00831 , B01F15/0085 , B01L3/505
摘要： Container and method for the mixing of media, in particular as a disposable container, which has at least one closable opening for the introduction and/or removal of the media and has a first sensor for measurement of at least one parameter of the mixture, in which at least one second sensor for measurement of the same parameter is arranged at a distance from the first sensor.
摘要翻译： 用于混合介质，特别是作为一次性容器的容器和方法，其具有用于引入和/或移除介质的至少一个可闭合的开口，并且具有用于测量混合物的至少一个参数的第一传感器， 其中用于测量相同参数的至少一个第二传感器布置在离第一传感器一定距离处。
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公开(公告)号：US20090314666A1
公开(公告)日：2009-12-24
申请号：US12226082
申请日：2007-04-12
IPC分类号： B65D25/00
CPC分类号： B01F15/0085 , B01F7/16 , B01F11/04 , B01F13/08 , B01F15/00896 , B01F2215/0032
摘要： Container system with one container (1) with a flexible partition, in particular a disposable container, that can be inserted into a carrying container that supports its lateral container partition (10) and in which liquid medium (8) that has been poured in can be mixed, with the carrying container (4) featuring at least one baffle (15, 15′, 15″, 15′″) on the internal support partition (11) that forms the lateral container partition.
摘要翻译： 具有一个容器（1）的容器系统，其具有柔性分隔物，特别是一次性容器，其可以插入到支撑其侧向容器隔板（10）的运载容器中，并且其中已经注入罐的液体介质（8） 与在内部支撑隔板（11）上具有至少一个挡板（15,15'，15“，15”）的承载容器（4）混合，形成侧向容器隔板。
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公开(公告)号：US20090075362A1
公开(公告)日：2009-03-19
申请号：US12226463
申请日：2007-04-24
申请人： Reinhard Baumfalk , Oscar-Werner Reif , Thomas Scheper , Daniel Riechers , Russell Hayden , Philip Nuzzo
发明人： Reinhard Baumfalk , Oscar-Werner Reif , Thomas Scheper , Daniel Riechers , Russell Hayden , Philip Nuzzo
IPC分类号： C12M3/00
CPC分类号： C12M23/28 , B01L3/505 , B01L2200/026 , B01L2200/04 , B01L2300/0627 , B01L2300/0645 , B01L2300/0654 , C12M23/00 , C12M23/14 , C12M41/00
摘要： The invention relates to a disposable bioreactor comprising a reversible, externally attachable sensor arrangement for measuring a physical variable of a contained medium. A sensor adapter (28) for receiving an electronic sensor arrangement (34, 38; 42; 44a, 44b, 46, 48) interacting with the medium cross-flowing the peripheral lines (14, 16, 18), by means of an inner delimiting surface (32a, 32b; 42; 44a, 44b, 46, 48) of the sensor adapter, is integrated into at least one of the peripheral lines (14, 16, 18) of the bioreactor which is used to supply or discharge the medium. Said sensor adapter (28) is mounted on the peripheral line (14, 16, 18) as an insert extending the peripheral line (14, 16, 18) and which can be cross-flown by the contained medium.
摘要翻译： 本发明涉及一种一次性生物反应器，其包括用于测量所容纳介质的物理变量的可逆的外部可附接的传感器装置。 一种传感器适配器（28），用于接收与所述介质相互作用的电子传感器装置（34,38; 42; 44a，44b，46,48），所述电子传感器装置通过内部 所述传感器适配器的限定表面（32a，32b; 42; 44a，44b，46,48）被整合到所述生物反应器的至少一个外围线路（14,16,18）中，所述外围线路用于提供或释放 中。 所述传感器适配器（28）作为插入件延伸外围线路（14,16,18）而安装在外围线路（14,16,18）上，并可由所容纳的介质交叉流动。
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