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    Synthetic membrane vesicles containing functionally active fusion peptides as drug delivery systems
    1.
    发明授权
    Synthetic membrane vesicles containing functionally active fusion peptides as drug delivery systems 失效
    含有功能活性融合肽作为药物递送系统的合成膜囊泡

    公开(公告)号：US6040167A

    公开(公告)日：2000-03-21

    申请号：US930593

    申请日：1992-11-02

    申请人： Reinhard Gluck ,  Peter Klein ,  Peter Herrmann ,  Ernst Rudolf Walti

    发明人： Reinhard Gluck ,  Peter Klein ,  Peter Herrmann ,  Ernst Rudolf Walti

    IPC分类号： A61K9/127 ,  A61K31/16 ,  A61K31/17 ,  A61K31/415 ,  A61K31/505 ,  A61K31/675 ,  A61K31/70 ,  A61K39/395 ,  A61K47/24 ,  A61K47/48 ,  A61P31/18 ,  A61P35/00 ,  C12N7/00 ,  C07K14/11

    CPC分类号： A61K9/1277 ,  A61K47/48523 ,  A61K47/48561 ,  A61K47/48823 ,  A61K9/1271

    摘要： The phospholipid bilayer contains at least one pharmaceutically active drug and comprises cell-specific markers on the membrane which have at least 90% biological activity when measured according to Luescher & Glueck, Antiviral Research 14, 39-50. In the membrane, the cholesterol content is preferably less than 2% by weight, the detergent content preferably less than 1 ppb. The vesicle diameter preferably is about 80 nm. the phospholipid in the membrane may comprise 70 to 95% by weight of phosphatidylcholine and preferably 10 to 20% by weight of phosphatidylethanolamine; preferably 6-8% by weight of a crosslinker, preferably of a sulfosuccinimidyl derivate, and at least one cell-specific fusion peptide are linked to the membrane. The vesicles are used for the preparation of pharmaceuticals against AIDS and carcinomas.

    摘要翻译： PCT No.PCT / EP92 / 00089 Sec。 371日期：1992年11月2日 102（e）日期1992年11月2日PCT提交1992年1月17日PCT公布。 出版物WO92 / 日期1992年8月20日当根据Luescher＆Glueck，Antiviral Research 14,39-50测量时，磷脂双层含有至少一种药物活性药物，并且包括膜上的细胞特异性标记物，其具有至少90％的生物活性。 在膜中，胆固醇含量优选小于2重量％，洗涤剂含量优选小于1ppb。 泡囊直径优选为约80nm。 膜中的磷脂可以包含70至95重量％的磷脂酰胆碱，优选10至20重量％的磷脂酰乙醇胺; 优选6-8重量％的交联剂，优选磺基琥珀酰亚胺基衍生物，和至少一个细胞特异性融合肽连接到膜。 囊泡用于制备抗艾滋病和癌症的药物。

    Cationic virosomes as transfer system for genetic material
    2.
    发明授权
    Cationic virosomes as transfer system for genetic material 失效
    阳离子病毒体作为遗传物质的转移系统

    公开(公告)号：US06210708B1

    公开(公告)日：2001-04-03

    申请号：US09414872

    申请日：1999-10-08

    申请人： Ernst Rudolf Walti ,  Reinhard Gluck ,  Peter Klein

    发明人： Ernst Rudolf Walti ,  Reinhard Gluck ,  Peter Klein

    IPC分类号： A61K9127

    CPC分类号： A61K9/1271 ,  A61K9/1272 ,  C12N15/88

    摘要： The present invention relates to a positively charged virosome for efficient delivery of genetic material to resting or proliferating mammalian cells in vitro and in vivo. The virosome membrane contains cationic and/or polycationic lipids, at least one viral fusion peptide and preferably at least one cell-specific marker, advantageously selected from the group consisting of monoclonal antibodies, antibody fragments F(ab′)2 and Fab′, cytokines, and growth factors, for a selective detection and binding of target cells. The invention further relates to a method for the manufacture of the novel virosomes and to applications thereof, particularly for the manufacture of pharmaceutical compositions to treat cancer or leukemia.

    摘要翻译： 本发明涉及用于在体外和体内有效地将遗传物质递送至休眠或增殖哺乳动物细胞的带正电荷的病毒颗粒。 病毒体膜包含阳离子和/或聚阳离子脂质，至少一种病毒融合肽，优选至少一种细胞特异性标记，有利地选自单克隆抗体，抗体片段F（ab'）2和Fab'，细胞因子 和生长因子，用于靶细胞的选择性检测和结合。 本发明还涉及用于制造新的病毒体及其应用的方法，特别是用于制备治疗癌症或白血病的药物组合物。

    Method of preparing lysozyme dimers
    3.
    发明授权
    Method of preparing lysozyme dimers 失效
    制备溶菌酶二聚体的方法

    公开(公告)号：US5989880A

    公开(公告)日：1999-11-23

    申请号：US197647

    申请日：1994-02-16

    申请人： Peter Herrmann ,  Peter Klein

    发明人： Peter Herrmann ,  Peter Klein

    IPC分类号： C12N9/36 ,  A61K37/48

    CPC分类号： C12N9/2462

    摘要： A method is provided for preparing a highly purified lysozyme dimer product which comprises dimerizing the lysozyme monomer with a suberimidate coupling reagent in a buffer solution adjusted to pH 10, stopping the dimerization at a given point by lowering the pH to 7 via addition of HCl or other suitable acid solution, and purifying the dimeric lysozyme by a series of elution steps carried out using ion exchange resin column chromatography. Monomeric lysozyme remaining undimerized by the initial dimerization step is recycled into the process in order to increase yield. The present system is advantageous in that large amounts of purified lysozyme dimer can be prepared efficiently and relatively inexpensively, and the lysozyme dimer so produced is useful in treating viral and/or bacterial diseases without causing the cytotoxic effects associated with the lysozyme monomer.

    摘要翻译： 提供了一种制备高纯度溶菌酶二聚体产物的方法，其包括将溶菌酶单体与缓冲溶液中的苏木精偶联试剂二聚化，调节至pH为10，通过加入HCl将pH降至7，停止给定点的二聚 其他合适的酸溶液，并通过使用离子交换树脂柱色谱法进行的一系列洗脱步骤纯化二聚溶菌酶。 通过初始二聚步骤保持未稳定化的单体溶菌酶被再循环到该方法中以提高产率。 本发明的系统的优点在于可以有效和相对廉价地制备大量纯化的溶菌酶二聚体，所产生的溶菌酶二聚体可用于治疗病毒和/或细菌疾病，而不会引起与溶菌酶单体相关的细胞毒性作用。

    Lysozyme dimer
    4.
    发明授权
    Lysozyme dimer 失效
    溶菌酶二聚体

    公开(公告)号：US06316236B1

    公开(公告)日：2001-11-13

    申请号：US09130810

    申请日：1998-08-07

    申请人： Peter Herrmann ,  Peter Klein

    发明人： Peter Herrmann ,  Peter Klein

    IPC分类号： C12N936

    CPC分类号： C12N9/2462

    摘要： A method is provided for preparing a highly purified lysozyme dimer product which comprises dimerizing the lysozyme monomer with a suberimidate coupling reagent in a buffer solution adjusted to pH 10, stopping the dimerization at a given point by lowering the pH to 7 via addition of HCl or other suitable acid solution, and purifying the dimeric lysozyme by a series of elution steps carried out using ion exchange resin column chromatography. Monomeric lysozyme remaining undimerized by the initial dimerization step is recycled into the process in order to increase yield. The present system is advantageous in that large amounts of purified lysozyme dimer can be prepared efficiently and relatively inexpensively, and the lysozyme dimer so produced is useful in treating viral and/or bacterial diseases without causing the cytotoxic effects associated with the lysozyme monomer.

    摘要翻译： 提供了一种制备高纯度溶菌酶二聚体产物的方法，其包括将溶菌酶单体与缓冲溶液中的苏木精偶联试剂二聚化，调节至pH为10，通过加入HCl将pH降至7，停止给定点的二聚 其他合适的酸溶液，并通过使用离子交换树脂柱色谱法进行的一系列洗脱步骤纯化二聚溶菌酶。 通过初始二聚步骤保持未稳定化的单体溶菌酶被再循环到该方法中以提高产率。 本发明的系统的优点在于可以有效和相对廉价地制备大量纯化的溶菌酶二聚体，所产生的溶菌酶二聚体可用于治疗病毒和/或细菌疾病，而不会引起与溶菌酶单体相关的细胞毒性作用。

    SYSTEM AND APPARATUS FOR MEASURING PARAMETER DATA ON IMPACT OF A GOLF CLUB FACE WITH A TARGET SURFACE
    5.
    发明申请
    SYSTEM AND APPARATUS FOR MEASURING PARAMETER DATA ON IMPACT OF A GOLF CLUB FACE WITH A TARGET SURFACE 有权
    用于测量高尔夫球杆面与目标表面碰撞的参数数据的系统和装置

    公开(公告)号：US20120295725A1

    公开(公告)日：2012-11-22

    申请号：US13473741

    申请日：2012-05-17

    申请人： Garry Peters ,  Peter Klein

    发明人： Garry Peters ,  Peter Klein

    IPC分类号： A63B57/00

    CPC分类号： G09B19/0038

    摘要： A system for measuring golf club parameter data on impact of a club face of a user's golf club with a target surface includes a static impact bag having a front facing surface for receiving an impact thereon with the club face, and a plurality of sensors for detecting force data upon impact of the club face with the front facing surface. The system includes a processor connected to sensors for receiving the force data and determining golf club parameter data related to the club face's impact with the front surface of the bag.

    摘要翻译： 用于测量使用者具有目标表面的使用者高尔夫球杆的球杆面的冲击的高尔夫球杆参数数据的系统包括：具有前表面的静态冲击袋，用于接收与球杆面的冲击;以及多个用于检测的传感器 在球杆面与前表面碰撞时强制数据。 该系统包括连接到传感器的处理器，用于接收力数据并确定与球杆表面与袋的前表面的冲击相关的高尔夫球杆参数数据。

    Method for producing biperiden IV
    6.
    发明授权
    Method for producing biperiden IV 失效
    双倍叶甙生产方法IV

    公开(公告)号：US07501519B2

    公开(公告)日：2009-03-10

    申请号：US10478384

    申请日：2002-05-17

    申请人： Markus Grosse ,  Klaus Martin Weber ,  Marco Thyes ,  Peter Klein ,  Elmar Vilsmaier

    发明人： Markus Grosse ,  Klaus Martin Weber ,  Marco Thyes ,  Peter Klein ,  Elmar Vilsmaier

    IPC分类号： C07D211/06

    CPC分类号： C07D295/088 ,  C07D295/108

    摘要： The invention relates to a method for producing biperiden by reacting exo-1-(bi-cyclo[2.2.1]hept-5-en-2-yl)-3-piperidino-1-propanone with a phenylmagnesium compound. According to the invention, exo-1-(bi-cyclo[2.2.1]hept-5-en-2-yl)-3-piperidino-1-propanone can be obtained by reacting the exo-ether of enol silylene of formula (IV) with a compound of N-methylenepiperidinium.

    摘要翻译： 本发明涉及通过使外-1-（双环[2.2.1]庚-5-烯-2-基）-3-哌啶子基-1-丙酮与苯基镁化合物反应制备双倍哌啶的方法。 根据本发明，外环1-（双环[2.2.1]庚-5-烯-2-基）-3-哌啶子基-1-丙酮可以通过使式 （Ⅳ）与N-亚甲基哌啶鎓的化合物反应。

    Tonneau cover for a truck box
    7.
    发明申请
    Tonneau cover for a truck box 失效
    Tonneau一个卡车箱盖

    公开(公告)号：US20070210608A1

    公开(公告)日：2007-09-13

    申请号：US11442652

    申请日：2006-05-30

    申请人： Peter Klein

    发明人： Peter Klein

    IPC分类号： B60P7/02

    CPC分类号： B60J7/1621

    摘要： A tonneau cover is arranged to mount on the truck box of a conventional pick up truck for enclosing the box. The cover comprises a pair of panels which are pivotally mounted on respective sides of the box. The panels extend laterally inward such that free ends of the panels engage each other in a closed position. The panels may be pivoted upward and secured in an upward orientation by a pair of rails connected between the free ends of the respective panels in an extended position of the panels. The panels form a pair of side walls in the extended position for supporting large cargo in the box. The rails form a rack in the extended position for supporting additional cargo thereon. A locking device secures the panels in the closed position while a plurality of struts provides additional support to the panels when pivoting the panels between the closed and extended positions.

    摘要翻译： 一个吨罩被布置成安装在用于封闭箱的传统拾取车的卡车箱上。 盖子包括一对可枢转地安装在箱体的相应侧面上的面板。 面板横向向内延伸，使得面板的自由端在闭合位置彼此接合。 面板可以向上枢转并且通过在面板的延伸位置中连接在相应面板的自由端之间的一对轨道向上固定。 面板在延伸位置形成一对侧壁，用于支撑箱子中的大货物。 轨道形成延伸位置的支架，用于在其上支撑附加货物。 锁定装置将面板固定在关闭位置，同时当多个支柱在闭合位置和延伸位置之间枢转面板时向板提供额外的支撑。