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1.发明申请BIOPOLYMER ANALYSIS METHOD, BIOPOLYMER ANALYZER, AND BIOPOLYMER ANALYSIS CHIP 审中-公开生物聚合物分析方法，生物聚合物分析仪和生物分析仪分析公开(公告)号：US20120312083A1
公开(公告)日：2012-12-13
申请号：US13579329
申请日：2011-02-23
申请人： Rena Akahori , Takashi Anazawa , Satoshi Ozawa , Yoshiaki Yazawa , Tomoyuki Sakai , Hideyuki Noda
发明人： Rena Akahori , Takashi Anazawa , Satoshi Ozawa , Yoshiaki Yazawa , Tomoyuki Sakai , Hideyuki Noda
IPC分类号： G01N33/48
CPC分类号： C12Q1/6869 , G01N21/6428 , G01N33/48721 , C12Q2565/631
摘要： There have been the following problems with sequence analysis using multiple nanopores: trapping a sample in the nanopores is not always 100% efficient and unnecessary time is spent to measure pores in which no sample has been trapped, resulting in low measurement efficiency. To address the problems, a labeling substance is boned to a sample, and the sample to which the labeling substance has been bonded is trapped in the nanopores. An apparatus for observing the labeling substance is used to observe the labeling substance and monitor whether or not the sample has been trapped in the nanopores. Measuring only nanopores in which the sample has been trapped allows the measurement efficiency to be improved.
摘要翻译： 使用多个纳米孔的序列分析存在以下问题：在纳米孔中捕获样品并不总是100％的有效性，并且花费不需要的时间来测量没有样品被捕获的孔隙，导致低的测量效率。 为了解决这些问题，将标记物质结合到样品中，并且标记物质已经结合的样品被捕获在纳米孔中。 用于观察标记物质的装置用于观察标记物质并监测样品是否已被捕获在纳米孔中。 仅测量样品已被捕获的纳米孔，可以提高测量效率。
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公开(公告)号：US20130176563A1
公开(公告)日：2013-07-11
申请号：US13812801
申请日：2011-07-21
申请人： Satoshi Ozawa , Takashi Anazawa , Rena Akahori , Satoshi Takahashi , Takeshi Ohura , Masashi Kiguchi
发明人： Satoshi Ozawa , Takashi Anazawa , Rena Akahori , Satoshi Takahashi , Takeshi Ohura , Masashi Kiguchi
IPC分类号： G01J3/02
CPC分类号： G01J3/0267 , B82Y5/00 , B82Y15/00 , C12Q1/6869 , G01N21/658 , G01N33/48721 , C12Q2565/631 , C12Q2565/632
摘要： The present invention provides a device and method for analyzing the characteristics of a biopolymer with excellent mechanical stability, high spatial resolution and sensitivity using a simple device construction. Specifically, the Raman scattered light of a biopolymer is measured and the properties of monomer units forming the biopolymer are analyzed by using a biopolymer property analysis chip (100a) characterized by comprising: a solid substrate (110); at least one nanopore (120) disposed in the solid substrate (110); and one or more electrically conductive thin films (130a, 130b) disposed on the solid substrate (110). The biopolymer property analysis chip (100a) is characterized in that the electrically conductive thin films (130a, 130b) are disposed partially on the solid substrate (110) where the nanopore (120) is formed and a biopolymer which has penetrated into the nanopore (120) is caused to generate Raman scattered light by means of irradiation with external light.
摘要翻译： 本发明提供一种使用简单的装置结构分析具有优异机械稳定性，高空间分辨率和灵敏度的生物聚合物的特性的装置和方法。 具体地，测量生物聚合物的拉曼散射光，并通过使用生物聚合物性质分析芯片（100a）分析形成生物聚合物的单体单元的性质，其特征在于包括：固体基材（110）; 设置在所述固体基板（110）中的至少一个纳米孔（120）; 以及设置在固体基板（110）上的一个或多个导电薄膜（130a，130b）。 生物聚合物性质分析芯片（100a）的特征在于，导电薄膜（130a，130b）部分地设置在形成有纳米孔（120）的固体基板（110）上，并且已经渗透到纳米孔中的生物聚合物 120）通过外部光照射产生拉曼散射光。
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公开(公告)号：US09562809B2
公开(公告)日：2017-02-07
申请号：US13812801
申请日：2011-07-21
申请人： Satoshi Ozawa , Takashi Anazawa , Rena Akahori , Satoshi Takahashi , Takeshi Ohura , Masashi Kiguchi
发明人： Satoshi Ozawa , Takashi Anazawa , Rena Akahori , Satoshi Takahashi , Takeshi Ohura , Masashi Kiguchi
IPC分类号： G01J3/02 , B82Y5/00 , B82Y15/00 , G01N33/487 , G01N21/65
CPC分类号： G01J3/0267 , B82Y5/00 , B82Y15/00 , C12Q1/6869 , G01N21/658 , G01N33/48721 , C12Q2565/631 , C12Q2565/632
摘要： The present invention provides a device and method for analyzing the characteristics of a biopolymer with excellent mechanical stability, high spatial resolution and sensitivity using a simple device construction. Specifically, the Raman scattered light of a biopolymer is measured and the properties of monomer units forming the biopolymer are analyzed by using a biopolymer property analysis chip (100a) characterized by comprising: a solid substrate (110); at least one nanopore (120) disposed in the solid substrate (110); and one or more electrically conductive thin films (130a, 130b) disposed on the solid substrate (110). The biopolymer property analysis chip (100a) is characterized in that the electrically conductive thin films (130a, 130b) are disposed partially on the solid substrate (110) where the nanopore (120) is formed and a biopolymer which has penetrated into the nanopore (120) is caused to generate Raman scattered light by means of irradiation with external light.
摘要翻译： 本发明提供一种使用简单的装置结构分析具有优异机械稳定性，高空间分辨率和灵敏度的生物聚合物的特性的装置和方法。 具体地，测量生物聚合物的拉曼散射光，并通过使用生物聚合物性质分析芯片（100a）分析形成生物聚合物的单体单元的性质，其特征在于包括：固体基材（110）; 设置在所述固体基板（110）中的至少一个纳米孔（120）; 以及设置在固体基板（110）上的一个或多个导电薄膜（130a，130b）。 生物聚合物性质分析芯片（100a）的特征在于，导电薄膜（130a，130b）部分地设置在形成有纳米孔（120）的固体基板（110）上，并且已经渗透到纳米孔中的生物聚合物 120）通过外部光照射产生拉曼散射光。
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公开(公告)号：US08197751B2
公开(公告)日：2012-06-12
申请号：US12976031
申请日：2010-12-22
申请人： Hideyuki Noda , Satoshi Ozawa , Masahiro Okanojo , Kenko Uchida
发明人： Hideyuki Noda , Satoshi Ozawa , Masahiro Okanojo , Kenko Uchida
摘要： An apparatus includes a system for guiding chemiluminescence and a system for preventing a variation in dark currents. The apparatus includes a first light shielding BOX having a sample container holder and a shutter unit therein, the shutter unit including a top plate which is partly formed by a movement of a plate member, and a second light shielding BOX having a photodetector therein. While a measurement is not implemented, the shutter unit is closed to block entrance of stray light to the photodetector, and while a measurement is implemented, the plate member is moved to open the shutter unit, and the tip of the photodetector is inserted into a through hole formed in the top plate, so that the distance between the bottom of the sample container and a sensitive area of the photodetector is reduced to several millimeters or less.
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公开(公告)号：US20110091355A1
公开(公告)日：2011-04-21
申请号：US12976031
申请日：2010-12-22
申请人： Hideyuki NODA , Satoshi Ozawa , Masahiro Okanojo , Kenko Uchida
发明人： Hideyuki NODA , Satoshi Ozawa , Masahiro Okanojo , Kenko Uchida
IPC分类号： G01N21/76
摘要： An apparatus includes a system for guiding chemiluminescence and a system for preventing a variation in dark currents. The apparatus includes a first light shielding BOX having a sample container holder and a shutter unit therein, the shutter unit including a top plate which is partly formed by a movement of a plate member, and a second light shielding BOX having a photodetector therein. While a measurement is not implemented, the shutter unit is closed to block entrance of stray light to the photodetector, and while a measurement is implemented, the plate member is moved to open the shutter unit, and the tip of the photodetector is inserted into a through hole formed in the top plate, so that the distance between the bottom of the sample container and a sensitive area of the photodetector is reduced to several millimeters or less.
摘要翻译： 一种装置包括用于引导化学发光的系统和用于防止暗电流变化的系统。 该装置包括：第一遮光箱，其具有样本容器保持器和其中的快门单元，所述快门单元包括部分地由板构件的运动形成的顶板和在其中具有光电检测器的第二遮光箱。 虽然没有实现测量，但是快门单元被关闭以阻止杂散光入射到光电检测器，并且在实现测量的同时，移动板构件以打开快门单元，并且将光电检测器的末端插入到 通孔形成在顶板中，使得样品容器的底部与光电检测器的敏感区域之间的距离减小到几毫米或更小。
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公开(公告)号：US07879290B2
公开(公告)日：2011-02-01
申请号：US12034880
申请日：2008-02-21
申请人： Hideyuki Noda , Satoshi Ozawa , Masahiro Okanojo , Kenko Uchida
发明人： Hideyuki Noda , Satoshi Ozawa , Masahiro Okanojo , Kenko Uchida
摘要： An apparatus includes a system for guiding chemiluminescence and a system for preventing a variation in dark currents. The apparatus includes a first light shielding BOX having a sample container holder and a shutter unit therein, the shutter unit including a top plate which is partly formed by a movement of a plate member and a second light shielding BOX having a photodetector therein. While a measurement is not implemented, the shutter unit is closed to block entrance of stray light to the photodetector, and while a measurement is implemented, the plate member is moved to open the shutter unit, and the tip of the photodetector is inserted into a through hole formed in the top plate, so that the distance between the bottom of the sample container and a sensitive area of the photodetector is reduced to several millimeters or less.
摘要翻译： 一种装置包括用于引导化学发光的系统和用于防止暗电流变化的系统。 该装置包括：第一遮光盒，其具有样品容器保持器和其中的快门单元，所述快门单元包括顶板，该顶板部分地通过板构件的运动和在其中具有光电检测器的第二遮光箱而形成。 虽然没有实现测量，但是快门单元被关闭以阻止杂散光入射到光电检测器，并且在实现测量的同时，移动板构件以打开快门单元，并且将光电检测器的末端插入到 通孔形成在顶板中，使得样品容器的底部与光电检测器的敏感区域之间的距离减小到几毫米或更小。
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公开(公告)号：US20080241871A1
公开(公告)日：2008-10-02
申请号：US12010497
申请日：2008-01-25
申请人： Masahiro Okanojo , Satoshi Ozawa , Hideyuki Noda , Masaaki Hirano
发明人： Masahiro Okanojo , Satoshi Ozawa , Hideyuki Noda , Masaaki Hirano
CPC分类号： G01N21/11 , G01N21/274 , G01N21/6428 , G01N2021/7786
摘要： The present invention provides a luminescence measuring method that can be accurately and quickly carried out while inhibiting a possible background associated with viable bacteria adhering to a nozzle or Adenosine Tri Phosphate remaining in the nozzle, and an apparatus for the method. The present invention uses a washing apparatus characterized by including a nozzle, a lysys solution, a luminescence reagent solution, and a detection section, as well as a relevant washing method and a relevant luminescence measuring method. To remove viable bacteria adhering to the nozzle, the nozzle is immersed in the lysys solution and then in the luminescence reagent solution. The detection section monitors luminescence occurring during a washing process.
摘要翻译： 本发明提供一种能够准确，快速地进行发光测定的方法，同时抑制与残留在喷嘴中的喷嘴或三磷酸腺苷的活菌相关的可能背景，以及该方法的装置。 本发明使用一种洗涤装置，其特征在于包括喷嘴，溶解液，发光试剂溶液和检测部分，以及相关的洗涤方法和相关的发光测量方法。 为了除去附着在喷嘴上的活细菌，将喷嘴浸入溶解液中，然后浸入发光试剂溶液中。 检测部分监视在洗涤过程中发生的发光。
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公开(公告)号：US20080261294A1
公开(公告)日：2008-10-23
申请号：US12034880
申请日：2008-02-21
申请人： Hideyuki Noda , Satoshi Ozawa , Masahiro Okanojo , Kenko Uchida
发明人： Hideyuki Noda , Satoshi Ozawa , Masahiro Okanojo , Kenko Uchida
摘要： An apparatus includes a system for guiding chemiluminescence and a system for preventing a variation in dark currents. The apparatus includes a first light shielding BOX having a sample container holder and a shutter unit therein, the shutter unit including a top plate which is partly formed by a movement of a plate member and a second light shielding BOX having a photodetector therein. While a measurement is not implemented, the shutter unit is closed to block entrance of stray light to the photodetector, and while a measurement is implemented, the plate member is moved to open the shutter unit and the tip of the photodetector is inserted into a through hole formed in the top plate, so that the distance between the bottom of the sample container and a sensitive area of the photodetector is reduced to several millimeters or less.
摘要翻译： 一种装置包括用于引导化学发光的系统和用于防止暗电流变化的系统。 该装置包括：第一遮光盒，其具有样品容器保持器和其中的快门单元，所述快门单元包括顶板，该顶板部分地通过板构件的运动和在其中具有光电检测器的第二遮光箱而形成。 虽然没有实现测量，但是关闭快门单元以阻止杂散光入射到光电检测器，并且在实施测量的同时，移动板构件以打开快门单元，并且将光电检测器的末端插入通孔 使得样品容器的底部与光电检测器的敏感区域之间的距离减小到几毫米或更小。
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9.发明授权Capturing carrier, capturing device, analysis system using the same, and method for capturing and testing microorganisms 有权捕获载体，捕获装置，使用其的分析系统以及捕获和测试微生物的方法公开(公告)号：US08628953B2
公开(公告)日：2014-01-14
申请号：US12292626
申请日：2008-11-21
申请人： Noe Osato , Ryusuke Gotoda , Satoshi Ozawa , Hideyuki Noda , Masahiro Okanojo
发明人： Noe Osato , Ryusuke Gotoda , Satoshi Ozawa , Hideyuki Noda , Masahiro Okanojo
IPC分类号： C12M1/00
CPC分类号： C12Q1/04 , G01N1/2208 , G01N1/2214 , G01N2001/4088
摘要： In order to make a process of capturing and testing air-borne microorganisms more convenient and quick, a capturing device which comprises the capturing carrier comprising a polymer which is in a gel phase at the time of capturing the microorganisms but undergoes a phase transition under heating to a sol phase at the temperature at or less than 40° C. (especially in the temperature range between 15° C. and 37° C.), and a vessel to contain the capturing carrier is used. Further, by comprising a test reagent in the polymer, the test reagent can be eluted upon said phase transition from a gel phase to a sol phase. By heating the capturing carrier, a phase transition to a sol phase can occur at or less than 40° C. As such, since recovery of microorganisms and addition of a test reagent to the microorganisms can be carried out simultaneously, the process can be simplified and the process time can be shortened.
摘要翻译： 为了更方便快捷地捕获和测试空气传播的微生物的过程，捕获装置包括捕获载体，捕获载体包含在捕获微生物时处于凝胶相的聚合物，但在加热下经历相变 在等于或低于40℃的温度下（特别是在15℃至37℃的温度范围内）的溶胶相，并且使用容纳捕获载体的容器。 此外，通过在聚合物中包含测试试剂，在从凝胶相到溶胶相的所述相转变时，可以洗脱测试试剂。 通过加热捕获载体，可以在40℃以下发生溶胶相的相变。因此，由于可以同时进行微生物的回收和微生物的添加试验，因此可以简化该过程 并且可以缩短处理时间。
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公开(公告)号：US08334144B2
公开(公告)日：2012-12-18
申请号：US13478527
申请日：2012-05-23
申请人： Hideyuki Noda , Satoshi Ozawa , Masahiro Okanojo , Kenko Uchida
发明人： Hideyuki Noda , Satoshi Ozawa , Masahiro Okanojo , Kenko Uchida
摘要： An apparatus includes a system for guiding chemiluminescence and a system for preventing a variation in dark currents. The apparatus includes a first light shielding BOX having a sample container holder and a shutter unit therein, the shutter unit including a top plate which is partly formed by a movement of a plate member, and a second light shielding BOX having a photodetector therein. While a measurement is not implemented, the shutter unit is closed to block entrance of stray light to the photodetector, and while a measurement is implemented, the plate member is moved to open the shutter unit, and the tip of the photodetector is inserted into a through hole formed in the top plate, so that the distance between the bottom of the sample container and a sensitive area of the photodetector is reduced to several millimeters or less.
摘要翻译： 一种装置包括用于引导化学发光的系统和用于防止暗电流变化的系统。 该装置包括：第一遮光箱，其具有样本容器保持器和其中的快门单元，所述快门单元包括部分地由板构件的运动形成的顶板和在其中具有光电检测器的第二遮光箱。 虽然没有实现测量，但是快门单元被关闭以阻止杂散光入射到光电检测器，并且在实现测量的同时，移动板构件以打开快门单元，并且将光电检测器的末端插入到 通孔形成在顶板中，使得样品容器的底部与光电检测器的敏感区域之间的距离减小到几毫米或更小。
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