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1.发明申请BIOPOLYMER ANALYSIS METHOD, BIOPOLYMER ANALYZER, AND BIOPOLYMER ANALYSIS CHIP 审中-公开生物聚合物分析方法，生物聚合物分析仪和生物分析仪分析公开(公告)号：US20120312083A1
公开(公告)日：2012-12-13
申请号：US13579329
申请日：2011-02-23
申请人： Rena Akahori , Takashi Anazawa , Satoshi Ozawa , Yoshiaki Yazawa , Tomoyuki Sakai , Hideyuki Noda
发明人： Rena Akahori , Takashi Anazawa , Satoshi Ozawa , Yoshiaki Yazawa , Tomoyuki Sakai , Hideyuki Noda
IPC分类号： G01N33/48
CPC分类号： C12Q1/6869 , G01N21/6428 , G01N33/48721 , C12Q2565/631
摘要： There have been the following problems with sequence analysis using multiple nanopores: trapping a sample in the nanopores is not always 100% efficient and unnecessary time is spent to measure pores in which no sample has been trapped, resulting in low measurement efficiency. To address the problems, a labeling substance is boned to a sample, and the sample to which the labeling substance has been bonded is trapped in the nanopores. An apparatus for observing the labeling substance is used to observe the labeling substance and monitor whether or not the sample has been trapped in the nanopores. Measuring only nanopores in which the sample has been trapped allows the measurement efficiency to be improved.
摘要翻译： 使用多个纳米孔的序列分析存在以下问题：在纳米孔中捕获样品并不总是100％的有效性，并且花费不需要的时间来测量没有样品被捕获的孔隙，导致低的测量效率。 为了解决这些问题，将标记物质结合到样品中，并且标记物质已经结合的样品被捕获在纳米孔中。 用于观察标记物质的装置用于观察标记物质并监测样品是否已被捕获在纳米孔中。 仅测量样品已被捕获的纳米孔，可以提高测量效率。
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公开(公告)号：US08040515B2
公开(公告)日：2011-10-18
申请号：US12051245
申请日：2008-03-19
IPC分类号： G01N21/25
CPC分类号： G01N21/6452 , G02B2207/113
摘要： In order to provide a fluorescence detection apparatus having a high sensitivity, a high processing capacity and a competitive edge in cost, the fluorescence detection apparatus according to this invention irradiate the sample with light so that the aspect ratio of the form of the irradiated region by light on the arrangement surface of the sample may be 1±0.1. The preferable form of irradiate region is not limited to one and varies to some extent depending on the item to be optimized. The form of irradiated region may be, for example, a circle, an equilateral triangle, a square, a regular hexagon and the like.
摘要翻译： 为了提供具有高灵敏度，高处理能力和成本方面的竞争优势的荧光检测装置，根据本发明的荧光检测装置用光照射样品，使得照射区域的形式的纵横比由 样品排列表面上的光可能为1±0.1。 照射区域的优选形式不限于一个，并且根据要优化的项目而在一定程度上变化。 照射区域的形式可以是例如圆形，等边三角形，正方形，正六边形等。
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公开(公告)号：US20080239311A1
公开(公告)日：2008-10-02
申请号：US12051245
申请日：2008-03-19
CPC分类号： G01N21/6452 , G02B2207/113
摘要： In order to provide a fluorescence detection apparatus having a high sensitivity, a high processing capacity and a competitive edge in cost, the fluorescence detection apparatus according to this invention irradiate the sample with light so that the aspect ratio of the form of the irradiated region by light on the arrangement surface of the sample may be 1±0.1. The preferable form of irradiate region is not limited to one and varies to some extent depending on the item to be optimized. The form of irradiated region may be, for example, a circle, an equilateral triangle, a square, a regular hexagon and the like.
摘要翻译： 为了提供具有高灵敏度，高处理能力和成本方面的竞争优势的荧光检测装置，根据本发明的荧光检测装置用光照射样品，使得照射区域的形式的纵横比由 样品排列表面上的光可能为1±0.1。 照射区域的优选形式不限于一个，并且根据要优化的项目而在一定程度上变化。 照射区域的形式可以是例如圆形，等边三角形，正方形，正六边形等。
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公开(公告)号：US07419578B2
公开(公告)日：2008-09-02
申请号：US10819182
申请日：2004-04-07
申请人： Tomoyuki Sakai , Takashi Anazawa
发明人： Tomoyuki Sakai , Takashi Anazawa
IPC分类号： G01N27/453
CPC分类号： G01N27/44782 , G01N27/44721
摘要： A capillary array electrophoresis which can measure fluorescence of a number of capillaries at a time with high sensitivity and can automatically analyze a sample sequentially. An end edge as a sample dissolution edge of a number of capillaries is connected to a polymer filling block having a mechanism of filling the polymer as an isolation medium inside a capillary. Fluorescence irradiated from an edge surface of an end edge portion of the capillary is detected through a detection window in which distance between an external surface and a detection surface is smaller than focal distance of an optical lens closest to a detection flat surface.
摘要翻译： 一种毛细管阵列电泳，可以高灵敏度一次测量多个毛细血管的荧光，并可以顺序自动分析样品。 作为多个毛细管的样品溶解边缘的端边缘连接到聚合物填充块，该聚合物填充块具有将聚合物填充在毛细管内的隔离介质的机构。 通过检测窗口检测从毛细管的端缘部分的边缘表面照射的荧光，其中外表面和检测表面之间的距离小于最接近检测平面的光学透镜的焦距。
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公开(公告)号：US20050211558A1
公开(公告)日：2005-09-29
申请号：US11006535
申请日：2004-12-08
IPC分类号： G01N27/447 , G01N27/453
CPC分类号： G01N27/44721
摘要： An end detection type capillary electrophoresis apparatus that enables high-speed electrophoresis at a high resolution. The capillary electrophoresis apparatus has an inner diameter of at least 20 μm and less than 80 μm, and satisfies the constraint that the inner diameter/glass outer diameter≧0.34. High fluorescence detection sensitivity is maintained and an analysis is carried out more quickly even as separation power improves.
摘要翻译： 一种能够以高分辨率进行高速电泳的末端检测型毛细管电泳装置。 毛细管电泳装置的内径为20μm以上且小于80μm，满足内径/玻璃外径> 0.34的限制。 维持高荧光检测灵敏度，即使分离能力提高，也能更快地进行分析。
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公开(公告)号：US08293191B2
公开(公告)日：2012-10-23
申请号：US11675749
申请日：2007-02-16
CPC分类号： B01L3/0244 , B01F7/00258 , B01F11/0071 , B01F11/0258 , B01F13/0071 , B01L3/0251 , B01L3/0255 , B01L3/50 , B01L2300/0845 , B01L2300/089 , B01L2300/12 , G01N21/03 , G01N21/64 , G01N2021/0346 , G01N2021/035 , G01N2035/1037 , G01N2035/1046
摘要： The invention provides a method in which an annular or spiral droplet holder formed of wire is used to hold a droplet in a state of being hung therefrom or being contained therein. A means for moving the droplet holder is added to the droplet holder to enable droplet transfer. To merge two droplets, they are brought into contact. To drip the droplet held by a droplet holder formed of wire, the droplet holder is deformed using an external force. A light path which passes through a droplet is set to enable optical measurement. The present invention enables inexpensive, simple droplet transfer. An inexpensive, simple configuration for handling droplets in the fields of chemical analysis, biochemical analysis, and automatic blood analysis can be realized according to the present invention.
摘要翻译： 本发明提供一种方法，其中使用由线形成的环形或螺旋液滴保持器将其悬挂在其中或被容纳在其中的状态。 用于移动液滴保持器的装置被添加到液滴保持器以使液滴转移。 为了合并两个液滴，它们被接触。 为了滴落由线形成的液滴保持器保持的液滴，液滴保持器使用外力而变形。 设置通过液滴的光路以进行光学测量。 本发明可实现廉价的简单的液滴转印。 根据本发明，可以实现用于处理化学分析，生化分析和自动血液分析领域中的液滴的廉价，简单的构造。
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公开(公告)号：US20070207064A1
公开(公告)日：2007-09-06
申请号：US11675749
申请日：2007-02-16
IPC分类号： B01L3/02
CPC分类号： B01L3/0244 , B01F7/00258 , B01F11/0071 , B01F11/0258 , B01F13/0071 , B01L3/0251 , B01L3/0255 , B01L3/50 , B01L2300/0845 , B01L2300/089 , B01L2300/12 , G01N21/03 , G01N21/64 , G01N2021/0346 , G01N2021/035 , G01N2035/1037 , G01N2035/1046
摘要： The invention provides a method in which an annular or spiral droplet holder formed of wire is used to hold a droplet in a state of being hung therefrom or being contained therein. A means for moving the droplet holder is added to the droplet holder to enable droplet transfer. To merge two droplets, they are brought into contact. To drip the droplet held by a droplet holder formed of wire, the droplet holder is deformed using an external force. A light path which passes through a droplet is set to enable optical measurement. The present invention enables inexpensive, simple droplet transfer. An inexpensive, simple configuration for handling droplets in the fields of chemical analysis, biochemical analysis, and automatic blood analysis can be realized according to the present invention.
摘要翻译： 本发明提供一种方法，其中使用由线形成的环形或螺旋液滴保持器将其悬挂在其中或被容纳在其中的状态。 用于移动液滴保持器的装置被添加到液滴保持器以使液滴转移。 为了合并两个液滴，它们被接触。 为了滴落由线形成的液滴保持器保持的液滴，液滴保持器使用外力而变形。 设置通过液滴的光路以进行光学测量。 本发明可实现廉价的简单的液滴转印。 根据本发明，可以实现用于处理化学分析，生化分析和自动血液分析领域中的液滴的廉价，简单的构造。
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公开(公告)号：US10365287B2
公开(公告)日：2019-07-30
申请号：US13148479
申请日：2010-03-01
申请人： Tomoyuki Sakai , Takeshi Fujita
发明人： Tomoyuki Sakai , Takeshi Fujita
IPC分类号： G01N33/68 , G01N33/487
摘要： Although analysis can be very quickly conducted at a low cost by a method for measuring a biopolymer using a nanopore, the accuracy of distinguishing the individual monopolymers constituting the biopolymer is low. To both ends of a biopolymer through a nanopore, molecules which are larger than the nanopore are attached and then the biopolymer is reciprocated by an external force to thereby perform repeated measurements.
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公开(公告)号：US08012327B2
公开(公告)日：2011-09-06
申请号：US11653332
申请日：2007-01-16
IPC分类号： G01N27/447
CPC分类号： G01N27/44704 , G01N27/44721
摘要： An object of the present invention is to provide a capillary electrophoresis apparatus in which simultaneity can be ensured between sensitivity and data acquisition to decrease a pull-up signal while spectral data acquisition is eliminated in each capillary exchange. The invention relates to a capillary electrophoresis apparatus in which capillary position shift is detected in each capillary exchange by detecting a capillary position. A capillary position measuring light source is provided in the capillary electrophoresis apparatus of the invention. The capillary is irradiated with light emitted from the capillary position measuring light source, a capillary image is detected with a two-dimensional detector, and thereby a position deviation of the capillary is determined. On the basis of the position deviation of the capillary, a data acquisition area is set in the two-dimensional detector, or a reference fluorescent light spectrum determined from the capillary at the standard position is corrected.
摘要翻译： 本发明的目的是提供一种毛细管电泳装置，其中在每个毛细管交换中消除光谱数据采集时，可以在灵敏度和数据采集之间确保同时性，以减小上拉信号。 本发明涉及一种毛细管电泳装置，其中通过检测毛细管位置在每个毛细管交换中检测毛细管位置偏移。 在本发明的毛细管电泳装置中设置毛细管位置测定光源。 用从毛细管位置测量光源发射的光照射毛细管，用二维检测器检测毛细管图像，从而确定毛细管的位置偏差。 基于毛细管的位置偏差，在二维检测器中设置数据获取区域，或者校正从标准位置处的毛细管确定的参考荧光光谱。
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公开(公告)号：US20060231400A1
公开(公告)日：2006-10-19
申请号：US11397890
申请日：2006-04-05
申请人： Ryoji Inaba , Tomoyuki Sakai , Motohiro Yamazaki , Takashi Gomi , Kazumichi Imai
发明人： Ryoji Inaba , Tomoyuki Sakai , Motohiro Yamazaki , Takashi Gomi , Kazumichi Imai
CPC分类号： G01N27/44721 , G01N27/44782
摘要： The present invention relates to detection of an emission spectrum by irradiating excitation light onto a plurality of electrophoretic paths and dispersing fluorescent light output from the electrophoretic paths in a direction approximately vertical to an electrophoretic direction. According to the invention, since an emission spectrum to be detected does not substantially change over time, it becomes possible to make observed emission spectrums completely correspond to various fluorescent dyes or various bases.
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