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公开(公告)号：US4959319A
公开(公告)日：1990-09-25
申请号：US311846
申请日：1989-02-17
IPC分类号： C12N5/071
CPC分类号： C12N5/0621
摘要： A process for establishing functional human corneal tissue consisting of an enhanced contact-inhibited endothelial monolayer derived from isolated human corneal endothelial cells with other integral corneal layers remaining intact. The process is divided into four integral parts.1. Isolation of human corneal endothelial cells from donor corneas.2. Establishment of a human corneal endothelial cell line which involves the establishment of primary cell cultures, proliferation, and continued maintenance and subculturing of these cells in vitro.3. The utilization of long term storage of isolated human corneal endothelial cells at -80.degree. C.4. Utilizing these isolated corneal endothelial cells from the above processes to enhance the corneal endothelial monolayer of human donor corneas to be used for penetrating keratoplasty.Two distinct methods of corneal endothelial enhancement may be utilized. In the first method, isolated human corneal endothelial cells are incorporated into the existing donor monolayer and re-establish a contact-inhibited functional monolayer, with a flattened, hexagonal configuration. In this procedure, the donor corneal endothelium remains intact and is supplemented with additional corneal endothelium. In the second method, isolated human corneal endothelial cells are seeded onto a denuded Descement's membrane and re-establish an intact cellular matrix, and form a contact-inhibited functional endothelial monolayer. In this procedure, the donor corneal endothelium is completely removed and replaced with new endothelial cells.
摘要翻译： 用于建立功能性人角膜组织的方法，其由来自分离的人角膜内皮细胞的增强的接触抑制内皮单层组成，其余整体角膜层保持完整。 该过程分为四个组成部分。 1.从供体角膜分离人角膜内皮细胞。 2.建立人类角膜内皮细胞系，其涉及在体外建立原代细胞培养物，增殖和持续维持和传代培养这些细胞。 3.在-80℃下利用长期保存的孤立人角膜内皮细胞。4.利用上述方法分离的角膜内皮细胞，增强用于穿透角膜移植术的人供体角膜角膜内皮单层。 可以使用两种不同的角膜内皮增强方法。 在第一种方法中，将孤立的人角膜内皮细胞掺入现有的供体单层中，并重新建立具有扁平六边形构型的接触抑制功能单层。 在此过程中，供体角膜内皮保持完整，并补充额外的角膜内皮。 在第二种方法中，将分离的人角膜内皮细胞接种到裸露的Descement膜上并重新建立完整的细胞基质，并形成接触抑制功能内皮单层。 在此过程中，供体角膜内皮被完全去除，并用新的内皮细胞代替。
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公开(公告)号：US4886786A
公开(公告)日：1989-12-12
申请号：US151480
申请日：1988-02-02
IPC分类号： A61K9/00
CPC分类号： A61K9/0048
摘要： An additive for intraocular irrigation solution or surgical solution which provides the anterior chamber and posterior chamber of the eye with protection during surgical procedures that require irrigation. The additive for the solutions is composed of a balanced salt solution containing dextrose supplemented with chondroitin sulfate. Other chemical compositions can supplement the additive.
摘要翻译： 用于眼内灌注溶液或手术溶液的添加剂，其在需要灌洗的外科手术过程中提供眼睛的前房和后房保护。 溶液的添加剂由含有补充硫酸软骨素的葡萄糖的平衡盐溶液组成。 其他化学成分可以补充添加剂。
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公开(公告)号：US5019084A
公开(公告)日：1991-05-28
申请号：US893785
申请日：1986-08-06
CPC分类号： A01N1/0273 , A01N1/02 , A01N1/0263 , A61B17/32 , A61F2/14 , A61F2/142
摘要： A corneal holder, amenable for use in both a corneal storage system and a corneal cutting system with minimal physical manipulation of the corneal tissue itself. The holder provides a passageway through the apertures of both a base member and a cap member that is configured to allow a cutting device to be passed into cutting contact with a predetermined portion of the corneal tissue. The holder allows radial orientation of the predetermined portion in order to limit astigmatism; as well as constriction of the periphery of the corneal tissue during storage in order to alleviate swelling of the tissue due to fluid absorption.
摘要翻译： 角膜保持器（10），适用于角膜存储系统和角膜切割系统，其角膜组织（16）自身的物理操作最小。 保持器提供穿过基部构件（12）和盖构件（14）的孔的通道，该构件构造成允许切割装置与角膜组织（16）的预定部分切割接触。 保持器允许预定部分的径向取向以限制散光; 以及在储存期间角膜组织（16）的周边的收缩，以便减轻由于流体吸收引起的组织肿胀。
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公开(公告)号：US5102653A
公开(公告)日：1992-04-07
申请号：US463485
申请日：1990-01-11
IPC分类号： A61K49/00
CPC分类号： A61K49/00 , Y10S514/912
摘要： This is a non-primate vitreal replacement process that quantitates the inflammatory response to viscoelastic agents or solutions. More specifically, the rabbit vitreal replacement process consists of the surgical replacement of a small volume of the central gel vitreous of the rabbit with a viscoelastic agent or solution, and measuring the invasion of white blood cells in the vitreous and anterior chamber at 48 hours post injection. Additional parameters measured are anterior chamber flare, haze and flare in the vitreal chamber, ocular fundus clarity, presence of iris congestion, lens involvement, corneal clarity, conjunctival congestion and conjunctival swelling. In this process, the rabbit is evaluated prior to injection of the viscoelastic agent or solution and 48 hours post injection. Each parameter is evaluated on a scale of 0 to 5. A non-inflammatory sample consists of a mean total score of equal to or less than one or a vitreal cell count less than 50 cells/mm.sup.3. This is an extremely sensitive system for detecting small amounts of inflammatory agents in viscoelastic agents or solutions. The test solution is rated as acceptable for veterinary or human use if a non-inflammatory response is obtained in this process.
摘要翻译： 这是非灵长类玻璃体替代过程，其定量为粘弹剂或溶液的炎症反应。 更具体地，兔子玻璃体替代过程包括用粘弹性剂或溶液手术置换少量兔中心凝胶玻璃体，并在48小时后测量玻璃体和前房中白细胞的侵入 注射。 测量的附加参数是玻璃体腔中的前房耀斑，浑浊和耀斑，眼底清晰度，虹膜充血的存在，镜片参与，角膜清晰度，结膜充血和结膜肿胀。 在该过程中，在注射粘弹剂或溶液之前和注射后48小时评价兔子。 每个参数以0至5的等级进行评估。非炎性样品由等于或小于1的平均总分数或小于50个细胞/ mm 3的玻璃体细胞计数组成。 这是一种非常敏感的系统，用于在粘弹剂或溶液中检测少量的炎症因子。 如果在该过程中获得非炎症反应，则测试溶液被评为兽医或人类可接受的。
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公开(公告)号：US4713375A
公开(公告)日：1987-12-15
申请号：US761406
申请日：1985-08-01
CPC分类号： C08L1/00
摘要： Viscoelastic solution including a buffered solution, 1-8% cellulose gum and 1-8% chondroitin sulfate, pH adjusted to 7.2-7.6 at a osmolality between 200-400 MOSM. The buffered solution can be HEPES buffered minimum essential media (MEM), phosphate buffer system (PBS), balanced salt solution, or TC199.
摘要翻译： 包括缓冲溶液，1-8％纤维素胶和1-8％硫酸软骨素的粘弹性溶液，在200-400MMM之间的重量摩尔渗透压浓度下将pH调节至7.2-7.6。 缓冲溶液可以是HEPES缓冲的最小必需培养基（MEM），磷酸盐缓冲液（PBS），平衡盐溶液或TC199。
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公开(公告)号：US4696917A
公开(公告)日：1987-09-29
申请号：US761407
申请日：1985-08-01
IPC分类号： A61K31/715 , A61K9/00 , A61K31/70
CPC分类号： A61K9/0048
摘要： An irrigation solution which provides the anterior and posterior chamber of the eye protection during surgical procedures that require irrigation. This irrigation solution is composed of a HEPES buffered Eagle's Minimum Essential Media (MEM) with Earle's Salts, without phenol red, supplemented with mixed isomers of 99% pure, chondroitin sulfate, MEM non-essential amino acids, 2-mercaptoethanol, and sodium pyruvate.
摘要翻译： 一种在需要灌洗的手术过程中提供眼睛保护的前房和后房的灌洗溶液。 该灌注溶液由具有Earle Salts的HEPES缓冲的Eagle's Minimum Essential Media（MEM），不含酚红，补充99％纯度的混合异构体，硫酸软骨素，MEM非必需氨基酸，2-巯基乙醇和丙酮酸钠 。
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公开(公告)号：US5385723A
公开(公告)日：1995-01-31
申请号：US765427
申请日：1991-09-25
IPC分类号： A61K49/00
CPC分类号： A61K49/00 , Y10S514/912
摘要： This is a non-primate vitreal replacement process that quantitates the inflammatory response to viscoelastic agents or solutions. More specifically, the rabbit vitreal replacement process consists of the surgical replacement of a small volume of the central gel vitreous of the rabbit with a viscoelastic agent or solution, and measuring the invasion of white blood cells in the vitreous and anterior chamber at 48 hours post injection. Additional parameters measured are anterior chamber flare, haze and flare in the vitreal chamber, ocular fundus clarity, presence of iris congestion, lens involvement, corneal clarity, conjunctival congestion and conjunctival swelling. In this process, the rabbit is evaluated prior to injection of the viscoelastic agent or solution and 48 hours post injection. Each parameter is evaluated on a scale of 0 to 5. A non-inflammatory sample consists of a mean total score of equal to or less than one or a vitreal cell count less than 50 cells/mm.sup.3. This is an extremely sensitive system for detecting small amounts of inflammatory agents in viscoelastic agents or solutions. The test solution is rated as acceptable for veterinary or human use if a non-inflammatory response is obtained in this process.
摘要翻译： 这是非灵长类玻璃体替代过程，其定量为粘弹剂或溶液的炎症反应。 更具体地，兔子玻璃体替代过程包括用粘弹性剂或溶液手术置换少量兔中心凝胶玻璃体，并在48小时后测量玻璃体和前房中白细胞的侵入 注射。 测量的附加参数是玻璃体腔中的前房耀斑，浑浊和耀斑，眼底清晰度，虹膜充血的存在，镜片参与，角膜清晰度，结膜充血和结膜肿胀。 在该过程中，在注射粘弹剂或溶液之前和注射后48小时评价兔子。 每个参数以0至5的等级进行评估。非炎性样品由等于或小于1的平均总分数或小于50个细胞/ mm 3的玻璃体细胞计数组成。 这是一种非常敏感的系统，用于在粘弹剂或溶液中检测少量的炎症因子。 如果在该过程中获得非炎症反应，则测试溶液被评为兽医或人类可接受的。
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公开(公告)号：US6153582A
公开(公告)日：2000-11-28
申请号：US186580
申请日：1998-11-05
申请人： Debra L. Skelnik
发明人： Debra L. Skelnik
CPC分类号： A01N1/02 , A01N1/0226 , C12N5/0621 , C12N2500/10 , C12N2500/32 , C12N2500/34 , C12N2500/36 , C12N2500/40 , C12N2501/01 , C12N2501/135 , C12N2501/392 , Y10S514/912
摘要： A defined serumfree medical solution for applications in Ophthalmology, that contains one or more cell nutrient supplements, and a growth factor(s) which maintains and enhances the preservation of eye tissues, including human corneal, retinal and corneal epithelial tissues at low to physiological temperatures (2.degree. C. to 38.degree. C.). This solution is composed of a defined aqueous nutrient and electrolyte solution, supplemented with a glycosaminoglycan(s), a deturgescent agent(s), an energy source(s), a buffer system(s), an antioxidant(s), membrane stabilizing agents, an antibiotic(s) and/or antimycotic agent(s), ATP or energy precursors, nutrient cell supplements, coenzymes and enzyme supplements, nucleotide precursors, hormonal supplements, non-essential amino acids, trace minerals, trace elements and a growth factor(s).
摘要翻译： 用于眼科应用的含有一种或多种细胞营养补充剂的定义的无血液医学溶液以及在低至生理温度下维持和增强眼组织（包括人角膜，视网膜和角膜上皮组织）的保存和增强的生长因子 （2℃〜38℃）。 该溶液由限定的水性营养物和电解质溶液组成，补充有糖胺聚糖，一种或多种防腐剂，能量源，缓冲系统，抗氧化剂，膜稳定剂 药剂，抗生素和/或抗真菌剂，ATP或能量前体，营养细胞补充剂，辅酶和酶补充剂，核苷酸前体，激素补充剂，非必需氨基酸，微量元素，微量元素和生长 因素。
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