-
1.发明申请Novel modular type II restriction endonuclease, cspci, and the use of modular endonucleases for generating endonucleases with new specificities 审中-公开新型模块化II型限制性内切核酸酶，cspci以及使用模块化内切核酸酶产生具有新特异性的核酸内切酶公开(公告)号：US20070166719A1
公开(公告)日：2007-07-19
申请号：US10593790
申请日：2005-03-23
申请人： Richard Morgan , Geoffrey Wilson , Keith Lunnen , Daniel Heiter , Jack Benner , Celine Nkenfou , Stephen Picone
发明人： Richard Morgan , Geoffrey Wilson , Keith Lunnen , Daniel Heiter , Jack Benner , Celine Nkenfou , Stephen Picone
CPC分类号： C12N9/22
摘要： A novel restriction endonuclease and methods of making the same are obtainable from either Citrobacter species 2144 (NEB#1398) or the recombinant stain Escherichia coli (NEB#1554) which cleaves at nt sequence 5′-CAANNNNNGTGG-3′ (SEQ ID NO:14) in double-stranded DNA molecules. The novel restriction endonuclease is a modular protein in which the specificity moiety is an independent module from the restriction-modification module.
摘要翻译： 新型限制性内切核酸酶及其制备方法可从柠檬酸杆菌物种2144（NEB＃1398）或以nt序列5'-CAANNNNNGTGG-3'（SEQ ID NO：1）切割的重组染色体大肠杆菌（NEB＃1554） 14）在双链DNA分子中。 新型限制性内切核酸酶是一种模块蛋白，其中特异性部分是来自限制性修饰模块的独立模块。
-
2.发明申请Composition and Method for Synthesizing a Deoxyribonucleotide Chain Using a Double Stranded Nucleic Acid Complex with a Thermostable Polymerase 审中-公开使用具有热稳定性聚合酶的双链核酸复合物合成脱氧核糖核苷酸链的组合物和方法公开(公告)号：US20110111462A1
公开(公告)日：2011-05-12
申请号：US12940307
申请日：2010-11-05
CPC分类号： C12Q1/6853 , C12Q2527/107 , C12Q2525/186 , C12Q2521/101 , C12Q2525/204
摘要： The present invention relates to the field of molecular biology, and more particular, to a nucleic acid construct for use in amplification processes. More precisely, the invention enhances the specificity of amplification of nucleic acids by means of a double stranded oligonucleotide modified with a molecule having the ability to prevent extension of the double stranded nucleic acid.
摘要翻译： 本发明涉及分子生物学领域，更具体地涉及用于扩增过程的核酸构建体。 更准确地说，本发明通过用具有防止双链核酸延伸的能力的分子修饰的双链寡核苷酸增强核酸扩增的特异性。
-
搜索结果
2 条记录 (耗时 0.024 秒)
