公开(公告)号：US20070166719A1

公开(公告)日：2007-07-19

申请号：US10593790

申请日：2005-03-23

申请人： Richard Morgan ,  Geoffrey Wilson ,  Keith Lunnen ,  Daniel Heiter ,  Jack Benner ,  Celine Nkenfou ,  Stephen Picone

发明人： Richard Morgan ,  Geoffrey Wilson ,  Keith Lunnen ,  Daniel Heiter ,  Jack Benner ,  Celine Nkenfou ,  Stephen Picone

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P21/06 ,  C12N9/22 ,  C12N15/74 ,  C12N1/21

CPC分类号： C12N9/22

摘要： A novel restriction endonuclease and methods of making the same are obtainable from either Citrobacter species 2144 (NEB#1398) or the recombinant stain Escherichia coli (NEB#1554) which cleaves at nt sequence 5′-CAANNNNNGTGG-3′ (SEQ ID NO:14) in double-stranded DNA molecules. The novel restriction endonuclease is a modular protein in which the specificity moiety is an independent module from the restriction-modification module.

摘要翻译： 新型限制性内切核酸酶及其制备方法可从柠檬酸杆菌物种2144（NEB＃1398）或以nt序列5'-CAANNNNNGTGG-3'（SEQ ID NO：1）切割的重组染色体大肠杆菌（NEB＃1554） 14）在双链DNA分子中。 新型限制性内切核酸酶是一种模块蛋白，其中特异性部分是来自限制性修饰模块的独立模块。

公开(公告)号：US07081358B2

公开(公告)日：2006-07-25

申请号：US10223074

申请日：2002-08-16

申请人： Daniel Heiter ,  Keith Lunnen ,  Geoffrey G. Wilson

发明人： Daniel Heiter ,  Keith Lunnen ,  Geoffrey G. Wilson

IPC分类号： C12N15/55 ,  C12N9/22

CPC分类号： C12N9/22

摘要： Methods are provided for converting into a sequence specific strand specific and location specific DNA nicking endonuclease, a restriction endonuclease that recognizes an asymmetric DNA sequence, the endonuclease having two catalytic sites and one or more single sequence specific DNA-binding domains. In one embodiment the method requires inactivating one of the catalytic sites of the restriction endonuclease. In another embodiment, the restriction endonuclease is a dimer having a first and second subunit each comprising a sequence specific DNA binding domain, a catalytic site and a dimerization domain. The nicking endonuclease is formed from combining one subunit having an inactivated catalytic site and a second subunit having an inactivated DNA binding domain. The nicking endonuclease may be converted into a restriction endonuclease by the addition of manganese cations in the digestion buffer.

公开(公告)号：US20080009036A1

公开(公告)日：2008-01-10

申请号：US11818298

申请日：2007-06-14

申请人： Keith Lunnen ,  John Greci ,  Geoffrey Wilson

发明人： Keith Lunnen ,  John Greci ,  Geoffrey Wilson

IPC分类号： C12P21/06 ,  C12N9/22 ,  C07H21/04 ,  C12N15/09

CPC分类号： C12N9/22 ,  C12N9/1007

摘要： An isolated DNA is provided which encodes a protein that is capable of binding to 5′GGTACC-3′, the isolated DNA being capable of hybridizing to SEQ ID NO:3 under stringent hybridization conditions. The isolated DNA may be alternatively characterized as coding for a protein having an amino acid sequence comprising SEQ ID NO:5 or by an amino acid sequence with an expectation value of less than E=e−02 in a BLAST search using SEQ ID NO:5. Vectors containing the isolated DNA and host cells expressing the vectors as well as a method for making recombinant Acc65I having the above properties are also provided.

摘要翻译： 提供分离的DNA，其编码能够结合5'GGTACC-3'的蛋白质，所述分离的DNA能够在严格的杂交条件下与SEQ ID NO：3杂交。 可以将分离的DNA替代地表征为编码具有包含SEQ ID NO：5的氨基酸序列的蛋白质，或编码具有小于E = e-O 2的期望值的氨基酸序列 使用SEQ ID NO：5进行BLAST检索。 还提供了含有表达载体的分离的DNA和宿主细胞的载体以及制备具有上述性质的重组Acc65I的方法。

公开(公告)号：US20050064433A1

公开(公告)日：2005-03-24

申请号：US10668047

申请日：2003-09-22

申请人： Keith Lunnen ,  Theodore Davis ,  Geoffrey Wilson

发明人： Keith Lunnen ,  Theodore Davis ,  Geoffrey Wilson

IPC分类号： C12N1/21 ,  C12N9/10 ,  C12N9/22 ,  C12N15/55 ,  C12Q1/68 ,  C07H21/04 ,  C12N15/74

CPC分类号： C12N9/1007 ,  C12N9/22

摘要： The present invention relates to: recombinant DNA encoding the SbfI restriction endonuclease as well as the SbfI methylase, and expression of the SbfI restriction endonuclease and SbfI methylase in E. coli cells containing the recombinant DNA; and methods for cloning the SbfI restriction gene (sbfIR) from Streptomyces species Bf-61 into E. coli by PCR. The method relied on primers based on DNA sequences predicted from amino acid sequences of the purified SbfI restriction endonuclease.

摘要翻译： 本发明涉及编码SbfI限制性内切核酸酶以及SbfI甲基化酶的重组DNA，以及含有重组DNA的大肠杆菌细胞中SbfI限制性内切核酸酶和SbfI甲基化酶的表达。 以及通过PCR将来自Streptomyces物种Bf-61的SbfI限制性基因（sbfIR）克隆到大肠杆菌中的方法。 该方法依赖于基于纯化的SbfI限制性内切核酸酶的氨基酸序列预测的DNA序列的引物。

公开(公告)号：US07795408B2

公开(公告)日：2010-09-14

申请号：US11818298

申请日：2007-06-14

申请人： Keith Lunnen ,  John Greci ,  Geoffrey Wilson

发明人： Keith Lunnen ,  John Greci ,  Geoffrey Wilson

IPC分类号： C07H21/02 ,  C07H21/04 ,  C12P21/06 ,  C12N1/20 ,  C12N15/74

CPC分类号： C12N9/22 ,  C12N9/1007

摘要： An isolated DNA is provided which encodes a protein that is capable of binding to 5′GGTACC-3′, the isolated DNA being capable of hybridizing to SEQ ID NO:3 under stringent hybridization conditions. The isolated DNA may be alternatively characterized as coding for a protein having an amino acid sequence comprising SEQ ID NO:5 or by an amino acid sequence with an expectation value of less than E=e−02 in a BLAST search using SEQ ID NO:5. Vectors containing the isolated DNA and host cells expressing the vectors as well as a method for making recombinant Acc65I having the above properties are also provided.

摘要翻译： 提供分离的DNA，其编码能够结合5'GGTACC-3'的蛋白质，所述分离的DNA能够在严格杂交条件下与SEQ ID NO：3杂交。 也可以将分离的DNA表征为在使用SEQ ID NO：5的BLAST搜索中编码具有包含SEQ ID NO：5的氨基酸序列或具有小于E = e-02的期望值的氨基酸序列的蛋白质。 5。 还提供了含有表达载体的分离的DNA和宿主细胞的载体以及制备具有上述性质的重组Acc65I的方法。

公开(公告)号：US5200337A

公开(公告)日：1993-04-06

申请号：US782515

申请日：1991-10-25

申请人： Carol Polisson ,  Derek Robinson ,  Keith Lunnen

发明人： Carol Polisson ,  Derek Robinson ,  Keith Lunnen

IPC分类号： C12N9/12 ,  C12N9/10 ,  C12N9/16 ,  C12N9/22 ,  C12N15/09 ,  C12N15/55 ,  C12R1/06

CPC分类号： C12N9/22 ,  C12N9/1007

摘要： The present invention provides a novel type II restriction endonuclease obtainable from Arthrobacter protophormiae. The endonuclease known as Apo I, recognizes the following nucleotide sequence and has a cleavage point indicated by the arrows: ##STR1## Also described is a process for obtaining Apo I from Arthrobacter protophormiae.

摘要翻译： 本发明提供了可从节杆菌（Arthrobacter protophormiae）获得的新型II型限制性内切核酸酶。 称为Apo I的内切核酸酶识别以下核苷酸序列，并具有由箭头指示的切割点：还描述了从原生节杆菌获得Apo I的方法。