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    Heterologous gene expression in lactococcus, and the expression products therefrom
    1.
    发明授权
    Heterologous gene expression in lactococcus, and the expression products therefrom 失效
    在乳球菌中的异源基因表达及其表达产物

    公开(公告)号：US06221648B1

    公开(公告)日：2001-04-24

    申请号：US08290995

    申请日：1994-10-27

    申请人： Richard William Falla Le Page ,  Jeremy Mark Wells ,  Peter William Wilson ,  Pamela Norton De Villareal

    发明人： Richard William Falla Le Page ,  Jeremy Mark Wells ,  Peter William Wilson ,  Pamela Norton De Villareal

    IPC分类号： C12N1574

    CPC分类号： C07K14/005 ,  A01N63/00 ,  A61K39/00 ,  C07K14/325 ,  C07K14/33 ,  C12N15/746 ,  C12N2740/16122

    摘要： Heterologous polypeptides are produced in lactococcus using a T7 or T7-like RNA polymerase gene under the control of an inducible promoter effective in a lactococcus host, and a promoter specific for said polymerase upstream of a coding sequence for the heterologous polypeptide. Thus, the promoter specific for the polymerase directs transcription of the coding sequence selectively as a result of expression of the polymerase. The heterologous polypeptide can be produced at high yield, and can be secreted. The polypeptide within the cell, being biologically active, can be delivered in the encapsulated form, e.g. as a medicament, vaccine or as an environmental pesticide.

    摘要翻译： 异源多肽在乳球菌中使用T7或T7样RNA聚合酶基因产生，所述T7或T7样RNA聚合酶基因在有效于乳球菌宿主的诱导型启动子的控制下，以及在异源多肽的编码序列上游的所述聚合酶特异的启动子。 因此，作为聚合酶表达的结果，聚合酶特异性启动子选择性地指导编码序列的转录。 异源多肽可以高产率生产，并可分泌。 具有生物活性的细胞内的多肽可以以包封的形式递送，例如， 作为药物，疫苗或环境农药。

    Clostridium perfringens vaccine
    7.
    发明授权
    Clostridium perfringens vaccine 失效
    产气荚膜梭菌疫苗

    公开(公告)号：US06610300B1

    公开(公告)日：2003-08-26

    申请号：US09100703

    申请日：1998-06-19

    申请人： Ruud Philip Antoon Maria Segers ,  Nicolas Robin Waterfield ,  Peer Lyng Frandsen ,  Jeremy Mark Wells

    发明人： Ruud Philip Antoon Maria Segers ,  Nicolas Robin Waterfield ,  Peer Lyng Frandsen ,  Jeremy Mark Wells

    IPC分类号： A61K3900

    CPC分类号： A61K39/08 ,  A61K39/00 ,  C07K14/33

    摘要： The present invention relates to detoxified immunogenic derivatives of Clostridium perfringens &bgr;-toxin or an immunogenic fragment thereof that have as a characteristic that they carry a mutation in the &bgr;-toxin amino acid sequence, not found in the wild-type &bgr;-toxin amino acid sequence. The invention also relates to genes encoding such &bgr;-toxins, as well as to expression systems expressing such &bgr;-toxins. Moreover, the invention relates to bacterial expression systems expressing a native &bgr;-toxin. Finally, the invention relates to vaccines based upon detoxified immunogenic derivatives of Clostridium perfringens &bgr;-toxin, and methods for the preparation of such vaccines.

    摘要翻译： 本发明涉及产气荚膜梭菌β-毒素或其免疫原性片段的解毒免疫原性衍生物，其具有在β-毒素氨基酸序列中携带突变的特征，在野生型β-毒素氨基酸中未发现 序列。 本发明还涉及编码这种β-毒素的基因以及表达这种β-毒素的表达系统。 此外，本发明涉及表达天然β-毒素的细菌表达系统。 最后，本发明涉及基于产气荚膜梭菌β-毒素的解毒免疫原性衍生物的疫苗，以及制备这种疫苗的方法。