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公开(公告)号：US5110908A
公开(公告)日：1992-05-05
申请号：US436092
申请日：1989-11-09
申请人： Robert A. Deich , Gary Zlotnick , Bruce Green
发明人： Robert A. Deich , Gary Zlotnick , Bruce Green
IPC分类号： A61K38/00 , A61K39/00 , C07K14/285 , C07K16/12 , C12N15/62 , C12N15/70 , C12Q1/68 , G01N33/569
CPC分类号： G01N33/56911 , C07K14/285 , C07K16/1242 , C12N15/625 , C12N15/70 , C12Q1/689 , A61K38/00 , A61K39/00 , C07K2319/00 , C07K2319/02 , C07K2319/035 , C07K2319/40 , Y10S435/82 , Y10S435/851 , Y10S530/806 , Y10S530/808 , Y10S530/825
摘要： Peptides and proteins related to an epitope comprising an outer membrane protein of Haemophilus influenzae are described. The peptides and proteins can be prepared by methods including novel and improved methods of puiification from H. influenzae cultures, and by recombinant DNA and chemical synthetic techniques. Additionally, recombinant vectors containing nucleotide sequences encoding PBOMP-1 and PBOMP-2 related peptides and proteins are also described. Recombinant vectors include plasmid DNA and viral DNA such as human viruses, animal viruses, insect viruses and bacteriophages that direct the expression of the PBOMP-1 and PBOMP-2 related peptides and proteins in appropriate host cells. The peptides, proteins and viruses both "live" and "inactivated" are used as immunogens in vaccine formulations to protect against H. influenzae infections. The peptides and proteins are also used as reagents in immunoassays as well as to prepare immunoglobulins for passive immunization. Use of the nucleotide sequences encoding the PBOMP related peptides and proteins in hybridization assays is also described.
摘要翻译： 描述了与包含流感嗜血杆菌的外膜蛋白的表位相关的肽和蛋白质。 肽和蛋白质可以通过包括来自流感嗜血杆菌培养物的新颖改良方法和重组DNA和化学合成技术的方法制备。 此外，还描述了含有编码PBOMP-1和PBOMP-2相关肽和蛋白质的核苷酸序列的重组载体。 重组载体包括质粒DNA和病毒DNA，例如人类病毒，动物病毒，昆虫病毒和噬菌体，其将PBOMP-1和PBOMP-2相关肽和蛋白质的表达指导在合适的宿主细胞中。 “活”和“灭活”的肽，蛋白质和病毒用作疫苗制剂中的免疫原，以防止流感嗜血杆菌感染。 肽和蛋白质也用作免疫测定中的试剂以及制备用于被动免疫的免疫球蛋白。 还描述了在杂交测定中使用编码PBOMP相关肽和蛋白质的核苷酸序列。
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公开(公告)号：US5196338A
公开(公告)日：1993-03-23
申请号：US480396
申请日：1990-02-15
IPC分类号： A61K38/00 , A61K39/00 , C07K14/285 , C07K16/12 , C12N15/62 , C12N15/70 , C12Q1/68 , C12Q1/70 , G01N33/569
CPC分类号： C07K14/285 , C07K16/1242 , C12N15/625 , C12N15/70 , C12Q1/689 , C12Q1/701 , G01N33/56911 , G01N33/56983 , A61K38/00 , A61K39/00 , C07K2317/34 , C07K2319/02 , C07K2319/035 , C07K2319/40
摘要： Peptides and proteins related to an epitope comprising an outer membrane protein of Haemophilus influenzae are described. The peptides and proteins can be prepared by methods including novel and improved methods of purification from H. influenzae cultures, and by recombinant DNA and chemical synthetic techniques. Additionally, recombinant vectors containing nucleotide sequences encoding PBOMP-1 and PBOMP-2 related peptides, proteins and fusion proteins are also described. Recombinant vectors include plasmid DNA and viral DNA such as human viruses, animal viruses, insect viruses and bacteriophages that direct the expression of the PBOMP-1 and PBOMP-2 related peptides, proteins, and fusion proteins in appropriate host cells. The peptides, proteins, fusion proteins and viruses both "live" and "inactivated" are used as immunogens in vaccine formulations to protect against H. influenzae infections. The peptides, proteins and fusion proteins are also used as reagents in immunoassays as well as to prepare immunoglobulins for passive immunization. Use of the nucleotide sequences encoding the PBOMP related peptides, proteins and fusion proteins in hybridization assays is also described.
摘要翻译： 描述了与包含流感嗜血杆菌的外膜蛋白的表位相关的肽和蛋白质。 肽和蛋白质可以通过包括来自流感嗜血杆菌培养物的新颖和改进的纯化方法以及重组DNA和化学合成技术的方法制备。 此外，还描述了含有编码PBOMP-1和PBOMP-2相关肽，蛋白质和融合蛋白的核苷酸序列的重组载体。 重组载体包括质粒DNA和病毒DNA，例如人类病毒，动物病毒，昆虫病毒和噬菌体，其在适当的宿主细胞中引导PBOMP-1和PBOMP-2相关肽，蛋白质和融合蛋白的表达。 “活”和“灭活”的肽，蛋白质，融合蛋白和病毒用作疫苗制剂中的免疫原，以防止流感嗜血杆菌感染。 肽，蛋白质和融合蛋白也用作免疫测定中的试剂，并且用于制备用于被动免疫的免疫球蛋白。 还描述了在杂交测定中使用编码PBOMP相关肽，蛋白质和融合蛋白的核苷酸序列。
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公开(公告)号：US5643747A
公开(公告)日：1997-07-01
申请号：US221750
申请日：1994-03-31
申请人： Steven M. Baker , Robert A. Deich
发明人： Steven M. Baker , Robert A. Deich
IPC分类号： C12N15/09 , C07H21/04 , C07K14/195 , C07K14/235 , C07K14/41 , C12N1/21 , C12N15/31 , C12P21/02 , C12R1/125 , C12R1/19 , C12N15/74
CPC分类号： C07K14/235
摘要： The invention relates to a cloned region of the Bordetella pertussis genome located 3' of the ptx operon encoding factors required for expression, assembly and secretion of pertussis holotoxin. Methods for obtaining increased levels of holotoxin production using homologous and heterologous hosts are also described.
摘要翻译： 本发明涉及百日咳杆菌基因组的克隆区域，位于编码百日咳全毒素表达，装配和分泌所需的ptx操纵子3'的3'位。 还描述了使用同源和异源宿主获得增加的全毒素产生水平的方法。
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公开(公告)号：US5098997A
公开(公告)日：1992-03-24
申请号：US239572
申请日：1988-09-01
IPC分类号： A61K38/00 , A61K39/00 , C07K14/285 , C07K16/12 , C12N15/62 , C12N15/70 , C12Q1/68 , C12Q1/70 , G01N33/569
CPC分类号： G01N33/56911 , C07K14/285 , C07K16/1242 , C12N15/625 , C12N15/70 , C12Q1/689 , C12Q1/701 , G01N33/56983 , A61K38/00 , A61K39/00 , C07K2317/34 , C07K2319/02 , C07K2319/035 , C07K2319/40 , Y10S435/851 , Y10S530/806 , Y10S530/825
摘要： Peptides and proteins related to an epitope comprising an outer membrane protein of Haemophilus influenzae are described. The peptides and proteins can be prepared by methods including novel and improved methods of purification from H. influenzae cultures, and by recombinant DNA and chemical synthetic techniques. Additionally, recombinant vectors containing nucleotide sequences encoding PBOMP-1 and PBOMP-2 related peptides, proteins and fusion proteins are also described. Recombinant vectors include plasmid DNA and viral DNA such as human viruses, animal viruses, insect viruses and bacteriophages that direct the expression of the PBOMP-1 and PBOMP-2 related peptides, proteins, and fusion proteins in appropriate host cells. The peptides, proteins, fusion proteins and viruses both "live" and "inactivated" are used as immunogens in vaccine formulations to protect against H. influenzae infections. The peptides, proteins and fusion proteins are also used as reagents in immunoassays as well as to prepare immunoglobulins for passive immunization. Use of the nucleotide sequences encoding the PBOMP related peptides, proteins and fusion proteins in hybridization assays is also described.
摘要翻译： 描述了与包含流感嗜血杆菌的外膜蛋白的表位相关的肽和蛋白质。 肽和蛋白质可以通过包括来自流感嗜血杆菌培养物的新颖和改进的纯化方法以及重组DNA和化学合成技术的方法制备。 此外，还描述了含有编码PBOMP-1和PBOMP-2相关肽，蛋白质和融合蛋白的核苷酸序列的重组载体。 重组载体包括质粒DNA和病毒DNA，例如人类病毒，动物病毒，昆虫病毒和噬菌体，其在适当的宿主细胞中引导PBOMP-1和PBOMP-2相关肽，蛋白质和融合蛋白的表达。 “活”和“灭活”的肽，蛋白质，融合蛋白和病毒用作疫苗制剂中的免疫原，以防止流感嗜血杆菌感染。 肽，蛋白质和融合蛋白也用作免疫测定中的试剂，并且用于制备用于被动免疫的免疫球蛋白。 还描述了在杂交测定中使用编码PBOMP相关肽，蛋白质和融合蛋白的核苷酸序列。
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公开(公告)号：US5108744A
公开(公告)日：1992-04-28
申请号：US434625
申请日：1989-11-09
IPC分类号： A61K38/00 , A61K39/00 , C07K14/285 , C07K16/12 , C12N15/62 , C12N15/70 , C12Q1/68 , G01N33/569
CPC分类号： C07K16/1242 , C07K14/285 , C12N15/625 , C12N15/70 , C12Q1/689 , G01N33/56911 , A61K38/00 , A61K39/00 , C07K2319/02 , C07K2319/035 , C07K2319/40
摘要： Peptides and proteins related to an epitope comprising an outer membrane protein of Haemophilus influenzae are described. The peptides and proteins can be prepared by methods includng novel and improved methods of purification from H. influenzae cultures, and by recombinant DNA and chemical synthetic techniques. Additionally, recombinant vectors containing nucleotide sequence encoding PBOMP-1 and PBOMP-2 related peptides and proteins are also described. Recombinant vectors include plasmid DNA and viral DNA such as human viruses, animal viruses, insect viruses and bacteriophages that direct the expression of the PBOMP-1 and PBOMP-2 related peptides and proteins in appropriate host cells. The peptides, proteins and viruses both "live" and "inactivated" are used as immunogens in vaccine formulations to protect against H. influenzae infections. The peptides and proteins are also used as reagents in immunoassays as well as to prepare immunoglobulins for passive immunization. Use of the nucleotide sequences encoding the PBOMP related peptides and proteins in hybridization assays is also described.
摘要翻译： 描述了与包含流感嗜血杆菌的外膜蛋白的表位相关的肽和蛋白质。 肽和蛋白质可以通过包括来自流感嗜血杆菌培养物的新颖和改进的纯化方法以及重组DNA和化学合成技术的方法制备。 此外，还描述了含有编码PBOMP-1和PBOMP-2相关肽和蛋白质的核苷酸序列的重组载体。 重组载体包括质粒DNA和病毒DNA，例如人类病毒，动物病毒，昆虫病毒和噬菌体，其将PBOMP-1和PBOMP-2相关肽和蛋白质的表达指导在合适的宿主细胞中。 “活”和“灭活”的肽，蛋白质和病毒在疫苗制剂中用作免疫原，以防止流感嗜血杆菌感染。 肽和蛋白质也用作免疫测定中的试剂以及制备用于被动免疫的免疫球蛋白。 还描述了在杂交测定中使用编码PBOMP相关肽和蛋白质的核苷酸序列。
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