公开(公告)号：US20080311677A1

公开(公告)日：2008-12-18

申请号：US12046964

申请日：2008-03-12

申请人： Robert C. Chin ,  Gregory F. Lopreato

发明人： Robert C. Chin ,  Gregory F. Lopreato

IPC分类号： G01N33/553

CPC分类号： G01N33/54326 ,  G01N33/58

摘要： A method of detecting a target substance includes contacting the target substance with a substrate. The substrate has a first receptor bound to the substrate. The target substance binds to the first receptor. The method further includes contacting a second receptor with the substrate. The second receptor is associated with the target substance. The second receptor is biotinylated. The method also includes contacting an anti-biotin antibody conjugated paramagnetic particle with the substrate.

摘要翻译： 检测目标物质的方法包括使目标物质与基底接触。 底物具有与底物结合的第一受体。 靶物质与第一受体结合。 该方法还包括使第二受体与基底接触。 第二受体与靶物质相关。 第二受体是生物素化的。 该方法还包括将抗生物素抗体共轭顺磁性颗粒与底物接触。

公开(公告)号：US08026071B2

公开(公告)日：2011-09-27

申请号：US12046964

申请日：2008-03-12

申请人： Robert C Chin ,  Gregory F. Lopreato

发明人： Robert C Chin ,  Gregory F. Lopreato

IPC分类号： G01N33/53

CPC分类号： G01N33/54326 ,  G01N33/58

摘要： A method of detecting a target substance includes contacting the target substance with a substrate. The substrate has a first receptor bound to the substrate. The target substance binds to the first receptor. The method further includes contacting a second receptor with the substrate. The second receptor is associated with the target substance. The second receptor is biotinylated. The method also includes contacting an anti-biotin antibody conjugated paramagnetic particle with the substrate.

摘要翻译： 检测目标物质的方法包括使目标物质与基底接触。 底物具有与底物结合的第一受体。 靶物质与第一受体结合。 该方法还包括使第二受体与基底接触。 第二受体与靶物质相关。 第二受体是生物素化的。 该方法还包括将抗生物素抗体共轭顺磁性颗粒与底物接触。

公开(公告)号：US07829502B2

公开(公告)日：2010-11-09

申请号：US12033522

申请日：2008-02-19

申请人： Gregory F. Lopreato

发明人： Gregory F. Lopreato

IPC分类号： C40B30/04 ,  C40B40/08

CPC分类号： C12N15/1048

摘要： A method for identifying aptamers that bind to target molecules may include contacting an oligonucleotide library with target molecule and digesting unbound oligonucleotides with one or more endonucleases, one or more exonucleases, or one or more endonucleases in combination with one or more exonucleases. A method for identifying aptamers may further include optionally subjecting selected aptamers to one or more rounds of selection under conditions of increased stringency. A method for identifying aptamers may include yet further amplifying selected aptamers. The described methods may be performed in a screen for identifying aptamers either alone or in combination with other methods typically employed in the art for selecting aptamers (such as, e.g., SELEX). Also contemplated herein are systems and kits for accomplishing the above.

摘要翻译： 用于鉴定与靶分子结合的适体的方法可包括使寡核苷酸文库与靶分子接触并用一种或多种核酸内切酶，一种或多种外切核酸酶或一种或多种内切核酸酶与一种或多种外切核酸酶组合消化未结合的寡核苷酸。 用于鉴定适体的方法还可以包括在增加严格性的条件下任选地使选定的适体进行一轮或多轮选择。 用于鉴定适体的方法可以包括进一步扩增所选择的适体。 所描述的方法可以在用于单独鉴定适体或与本领域通常用于选择适体（例如SELEX）的其它方法组合的筛选中进行。 本文还考虑用于实现上述的系统和套件。

公开(公告)号：US20100152056A1

公开(公告)日：2010-06-17

申请号：US12033522

申请日：2008-02-19

申请人： Gregory F. Lopreato

发明人： Gregory F. Lopreato

IPC分类号： C40B30/04 ,  C40B40/08

CPC分类号： C12N15/1048

摘要： A method for identifying aptamers that bind to target molecules may include contacting an oligonucleotide library with target molecule and digesting unbound oligonucleotides with one or more endonucleases, one or more exonucleases, or one or more endonucleases in combination with one or more exonucleases. A method for identifying aptamers may further include optionally subjecting selected aptamers to one or more rounds of selection under conditions of increased stringency. A method for identifying aptamers may include yet further amplifying selected aptamers. The described methods may be performed in a screen for identifying aptamers either alone or in combination with other methods typically employed in the art for selecting aptamers (such as, e.g., SELEX). Also contemplated herein are systems and kits for accomplishing the above.

摘要翻译： 用于鉴定与靶分子结合的适体的方法可包括使寡核苷酸文库与靶分子接触并用一种或多种核酸内切酶，一种或多种外切核酸酶或一种或多种内切核酸酶与一种或多种外切核酸酶组合消化未结合的寡核苷酸。 用于鉴定适体的方法还可以包括在增加严格性的条件下任选地使选定的适体进行一轮或多轮选择。 用于鉴定适体的方法可以包括进一步扩增所选择的适体。 所描述的方法可以在用于单独鉴定适体或与本领域通常用于选择适体（例如SELEX）的其它方法组合的筛选中进行。 本文还考虑用于实现上述的系统和套件。