公开(公告)号：US08026071B2

公开(公告)日：2011-09-27

申请号：US12046964

申请日：2008-03-12

申请人： Robert C Chin ,  Gregory F. Lopreato

发明人： Robert C Chin ,  Gregory F. Lopreato

IPC分类号： G01N33/53

CPC分类号： G01N33/54326 ,  G01N33/58

摘要： A method of detecting a target substance includes contacting the target substance with a substrate. The substrate has a first receptor bound to the substrate. The target substance binds to the first receptor. The method further includes contacting a second receptor with the substrate. The second receptor is associated with the target substance. The second receptor is biotinylated. The method also includes contacting an anti-biotin antibody conjugated paramagnetic particle with the substrate.

摘要翻译： 检测目标物质的方法包括使目标物质与基底接触。 底物具有与底物结合的第一受体。 靶物质与第一受体结合。 该方法还包括使第二受体与基底接触。 第二受体与靶物质相关。 第二受体是生物素化的。 该方法还包括将抗生物素抗体共轭顺磁性颗粒与底物接触。

公开(公告)号：US20110194979A1

公开(公告)日：2011-08-11

申请号：US13024227

申请日：2011-02-09

申请人： Robert C. Chin ,  Ronald E. Ham

发明人： Robert C. Chin ,  Ronald E. Ham

IPC分类号： G01N30/00 ,  G01N33/00

CPC分类号： G01N33/54326 ,  G01N33/58

摘要： An analyte detection system includes a detector situated close to a well of a substrate. The well includes conjugated paramagnetic beads. The detection system also includes a magnetic field generator that provides an oscillating magnetic field in the well and the detector, an oscillator circuit coupled to the detector, and a circuit coupled to the detector that detect the conjugated paramagnetic beads. A method includes applying a magnetic field to well of a substrate with conjugated paramagnetic beads, alternating the polarity of the magnetic field, detecting a waveform associated with the alternating magnetic field, and associating the waveform with the quantity of conjugated paramagnetic beads. An analyte detection kit includes a substrate with an attached antibody that is reactive to the analyte, a conjugated paramagnetic particle, and a conjugated paramagnetic particle detector.

摘要翻译： 分析物检测系统包括靠近基底阱的检测器。 该井包括共轭顺磁珠。 检测系统还包括一个磁场发生器，它在阱和检测器中提供振荡磁场，耦合到检测器的振荡器电路，以及耦合到检测器的电路，其检测共轭顺磁珠。 一种方法包括将磁场施加到具有共轭顺磁珠的衬底的阱中，交替磁场的极性，检测与交变磁场相关联的波形，以及将波形与共轭顺磁珠的量相关联。 分析物检测试剂盒包括具有与分析物反应的附着抗体的底物，共轭顺磁性颗粒和共轭顺磁性颗粒检测器。

公开(公告)号：US5981957A

公开(公告)日：1999-11-09

申请号：US958779

申请日：1997-10-27

申请人： Tommy C. Cruce ,  William H. Hallidy ,  Robert C. Chin

发明人： Tommy C. Cruce ,  William H. Hallidy ,  Robert C. Chin

IPC分类号： G01J3/433 ,  G01J3/44 ,  G01N21/64

CPC分类号： G01J3/4406 ,  G01J3/4338 ,  G01N21/6408

摘要： The present invention additionally comprises a method and apparatus for generating and mixing signals for frequency-domain lifetime and spectral fluorometry. The present invention comprises a plurality of signal generators that generate a plurality of signals where the signal generators modulate the amplitude and/or the frequency of the signals. The present invention uses one of these signals to drive an excitation signal that the present invention then directs and transmits at a target mixture, which absorbs the energy from the excitation signal. The property of fluorescence causes the target mixture to emit an emitted signal that the present invention detects with a signal detector. The present invention uses a plurality of mixers to produce a processor reference signal and a data signal. The present invention then uses a processor to compare the processor reference signal with the data signal by analyzing the differences in the phase and the differences in the amplitude between the two signals. The processor then extracts the fluorescence lifetime and fluorescence spectrum of the emitted signal from the phase and amplitude information using a chemometric analysis.

摘要翻译： 本发明还包括用于产生和混合用于频域寿命和频谱荧光测定的信号的方法和装置。 本发明包括多个信号发生器，其产生多个信号，其中信号发生器调制信号的幅度和/或频率。 本发明使用这些信号中的一个来驱动激励信号，本发明然后在从激励信号吸收能量的目标混合物引导和发射。 荧光的性质使目标混合物发射本发明用信号检测器检测的发射信号。 本发明使用多个混频器来产生处理器参考信号和数据信号。 本发明然后使用处理器来通过分析相位差和两个信号之间的幅度差异来比较处理器参考信号与数据信号。 然后处理器使用化学计量分析从相位和振幅信息中提取发射信号的荧光寿命和荧光光谱。

公开(公告)号：US5955737A

公开(公告)日：1999-09-21

申请号：US958413

申请日：1997-10-27

申请人： William H. Hallidy ,  Robert C. Chin

发明人： William H. Hallidy ,  Robert C. Chin

IPC分类号： G01N21/64

CPC分类号： G01N21/6408

摘要： The present invention is a system for chemometric analysis for the extraction of the individual component fluorescence spectra and fluorescence lifetimes from a target mixture. The present invention combines a processor with an apparatus for generating an excitation signal to transmit at a target mixture and an apparatus for detecting the emitted signal from the target mixture. The present invention extracts the individual fluorescence spectrum and fluorescence lifetime measurements from the frequency and wavelength data acquired from the emitted signal. The present invention uses an iterative solution that first requires the initialization of several decision variables and the initial approximation determinations of intermediate matrices. The iterative solution compares the decision variables for convergence to see if further approximation determinations are necessary. If the solution converges, the present invention then determines the reduced best fit error for the analysis of the individual fluorescence lifetime and the fluorescence spectrum before extracting the individual fluorescence lifetime and fluorescence spectrum from the emitted signal of the target mixture.

摘要翻译： 本发明是用于从目标混合物中提取各组分荧光光谱和荧光寿命的化学计量分析系统。 本发明将处理器与用于产生在目标混合物上发射的激励信号的装置和用于检测来自目标混合物的发射信号的装置相结合。 本发明从从发射信号中获取的频率和波长数据中提取各个荧光光谱和荧光寿命测量值。 本发明使用迭代解决方案，其首先需要初始化几个决策变量和中间矩阵的初始近似确定。 迭代解决方案比较收敛的决策变量，以查看是否需要进一步的近似确定。 如果溶液收敛，则本发明随后根据目标混合物的发射信号，提取单个荧光寿命和荧光光谱，确定用于分析各个荧光寿命和荧光光谱的降低的最佳拟合误差。

公开(公告)号：US5737085A

公开(公告)日：1998-04-07

申请号：US822556

申请日：1997-03-19

申请人： Byron G. Zollars ,  Robert C. Chin ,  Gary B. McMillian ,  Tommy C. Cruce ,  William H. Hallidy

发明人： Byron G. Zollars ,  Robert C. Chin ,  Gary B. McMillian ,  Tommy C. Cruce ,  William H. Hallidy

IPC分类号： G01B11/02 ,  G01S17/36 ,  G01B11/24

CPC分类号： G01B11/026 ,  G01S17/36

摘要： Apparatus and method for measuring the relative distance between different areas on a remote surface or displacements of a selected area of a remote surface with respect to a measuring apparatus are provided. A laser beam is modulated at an RF frequency and focused on a selected area. The selected area is scanned by movement of a mirror and at each selected area the phase difference is measured. Digital data processing of signals derived from the source and return beams is used to provide high precision. For detecting or measuring vibration or displacement, an analog signal is obtained from a low-pass filter.

摘要翻译： 提供了用于测量远程表面上的不同区域之间的相对距离或远离表面的选定区域相对于测量装置的位移的装置和方法。 激光束以RF频率被调制并且聚焦在所选择的区域上。 通过镜子的移动扫描所选择的区域，并且在每个选择的区域测量相位差。 来自源和返回光束的信号的数字数据处理用于提供高精度。 为了检测或测量振动或位移，从低通滤波器获得模拟信号。

公开(公告)号：US08927260B2

公开(公告)日：2015-01-06

申请号：US13024227

申请日：2011-02-09

申请人： Robert C. Chin ,  Ronald E. Ham

发明人： Robert C. Chin ,  Ronald E. Ham

IPC分类号： G01N33/553 ,  G01N33/543 ,  G01N33/58

CPC分类号： G01N33/54326 ,  G01N33/58

摘要： An analyte detection system includes a detector situated close to a well of a substrate. The well includes conjugated paramagnetic beads. The detection system also includes a magnetic field generator that provides an oscillating magnetic field in the well and the detector, an oscillator circuit coupled to the detector, and a circuit coupled to the detector that detect the conjugated paramagnetic beads. A method includes applying a magnetic field to well of a substrate with conjugated paramagnetic beads, alternating the polarity of the magnetic field, detecting a waveform associated with the alternating magnetic field, and associating the waveform with the quantity of conjugated paramagnetic beads. An analyte detection kit includes a substrate with an attached antibody that is reactive to the analyte, a conjugated paramagnetic particle, and a conjugated paramagnetic particle detector.

摘要翻译： 分析物检测系统包括靠近基底阱的检测器。 该井包括共轭顺磁珠。 检测系统还包括一个磁场发生器，它在阱和检测器中提供振荡磁场，耦合到检测器的振荡器电路，以及耦合到检测器的电路，其检测共轭顺磁珠。 一种方法包括将磁场施加到具有共轭顺磁珠的衬底的阱中，交替磁场的极性，检测与交变磁场相关联的波形，以及将波形与共轭顺磁珠的量相关联。 分析物检测试剂盒包括具有与分析物反应的附着抗体的底物，共轭顺磁性颗粒和共轭顺磁性颗粒检测器。

公开(公告)号：US20120022377A1

公开(公告)日：2012-01-26

申请号：US13182185

申请日：2011-07-13

申请人： Robert C. Chin ,  Sridhar Madala ,  Graham Patrick Sattler

发明人： Robert C. Chin ,  Sridhar Madala ,  Graham Patrick Sattler

IPC分类号： A61B8/00

CPC分类号： A61B8/06 ,  A61B8/0808 ,  A61B8/42 ,  A61B8/4254 ,  A61B8/4461 ,  A61B8/4472

摘要： The disclosure is directed to a transcranial Doppler probe. The transcranial Doppler probe includes a spherical bearing, a piezoelectric transducer pivotally attached to the spherical bearing, and first and second rods coupled to the piezoelectric transducer. The first rod is configured to pivot the piezoelectric transducer around a first pivot axis and the second rod is configured to pivot the piezoelectric transducer around a second pivot axis.

摘要翻译： 本公开涉及经颅多普勒探针。 经颅多普勒探针包括球面轴承，枢转地附接到球面轴承的压电换能器，以及耦合到压电换能器的第一和第二杆。 第一杆构造成围绕第一枢转轴线枢转压电换能器，并且第二杆构造成围绕第二枢转轴线枢转压电换能器。

公开(公告)号：US08622912B2

公开(公告)日：2014-01-07

申请号：US13182185

申请日：2011-07-13

申请人： Robert C. Chin ,  Sridhar Madala ,  Graham Patrick Sattler

发明人： Robert C. Chin ,  Sridhar Madala ,  Graham Patrick Sattler

IPC分类号： A61B8/00

CPC分类号： A61B8/06 ,  A61B8/0808 ,  A61B8/42 ,  A61B8/4254 ,  A61B8/4461 ,  A61B8/4472

摘要： The disclosure is directed to a transcranial Doppler probe. The transcranial Doppler probe includes a spherical bearing, a piezoelectric transducer pivotally attached to the spherical bearing, and first and second rods coupled to the piezoelectric transducer. The first rod is configured to pivot the piezoelectric transducer around a first pivot axis and the second rod is configured to pivot the piezoelectric transducer around a second pivot axis.

摘要翻译： 本公开涉及经颅多普勒探针。 经颅多普勒探针包括球面轴承，枢转地附接到球面轴承的压电换能器，以及耦合到压电换能器的第一和第二杆。 第一杆构造成围绕第一枢转轴线枢转压电换能器，并且第二杆构造成围绕第二枢转轴线枢转压电换能器。

公开(公告)号：US20080311677A1

公开(公告)日：2008-12-18

申请号：US12046964

申请日：2008-03-12

申请人： Robert C. Chin ,  Gregory F. Lopreato

发明人： Robert C. Chin ,  Gregory F. Lopreato

IPC分类号： G01N33/553

CPC分类号： G01N33/54326 ,  G01N33/58

摘要： A method of detecting a target substance includes contacting the target substance with a substrate. The substrate has a first receptor bound to the substrate. The target substance binds to the first receptor. The method further includes contacting a second receptor with the substrate. The second receptor is associated with the target substance. The second receptor is biotinylated. The method also includes contacting an anti-biotin antibody conjugated paramagnetic particle with the substrate.

摘要翻译： 检测目标物质的方法包括使目标物质与基底接触。 底物具有与底物结合的第一受体。 靶物质与第一受体结合。 该方法还包括使第二受体与基底接触。 第二受体与靶物质相关。 第二受体是生物素化的。 该方法还包括将抗生物素抗体共轭顺磁性颗粒与底物接触。

公开(公告)号：USRE39537E1

公开(公告)日：2007-04-03

申请号：US10035461

申请日：2001-11-09

申请人： Tommy Clay Cruce ,  William H. Hallidy ,  Robert C. Chin

发明人： Tommy Clay Cruce ,  William H. Hallidy ,  Robert C. Chin

IPC分类号： G01N21/64

CPC分类号： G01J3/4406 ,  G01J3/4338 ,  G01N21/6408

摘要： The present invention additionally comprises a method and apparatus for generating and mixing signals for frequency-domain lifetime and spectral fluorometry. The present invention comprises a plurality of signal generators that generate a plurality of signals where the signal generators modulate the amplitude and/or the frequency of the signals. The present invention uses one of these signals to drive an excitation signal that the present invention then directs and transmits at a target mixture, which absorbs the energy from the excitation signal. The property of fluorescence causes the target mixture to emit an emitted signal that the present invention detects with a signal detector. The present invention uses a plurality of mixers to produce a processor reference signal and a data signal. The present invention then uses a processor to compare the processor reference signal with the data signal by analyzing the differences in the phase and the differences in the amplitude between the two signals. The processor then extracts the fluorescence lifetime and fluorescence spectrum of the emitted signal from the phase and amplitude information using a chemometric analysis.

摘要翻译： 本发明还包括用于产生和混合用于频域寿命和频谱荧光测定的信号的方法和装置。 本发明包括多个信号发生器，其产生多个信号，其中信号发生器调制信号的幅度和/或频率。 本发明使用这些信号中的一个来驱动激励信号，本发明然后在从激励信号吸收能量的目标混合物引导和发射。 荧光的性质使目标混合物发射本发明用信号检测器检测的发射信号。 本发明使用多个混频器来产生处理器参考信号和数据信号。 本发明然后使用处理器来通过分析相位差和两个信号之间的幅度差异来比较处理器参考信号与数据信号。 然后处理器使用化学计量分析从相位和振幅信息中提取发射信号的荧光寿命和荧光光谱。