公开(公告)号：US4851356A

公开(公告)日：1989-07-25

申请号：US72802

申请日：1987-07-14

申请人： Robert E. Canfield ,  Elmo G. Armstrong ,  Paul H. Ehrlich ,  Steven Birken

发明人： Robert E. Canfield ,  Elmo G. Armstrong ,  Paul H. Ehrlich ,  Steven Birken

IPC分类号： G01N33/574 ,  G01N33/577 ,  G01N33/76

CPC分类号： G01N33/76 ,  G01N33/57476 ,  G01N33/577 ,  Y10S436/804 ,  Y10S436/813 ,  Y10S436/814 ,  Y10S436/818 ,  Y10S436/823

摘要： An immunoassay for detecting and measuring hCG in a sample includes an antibody directed to the carboxy terminal portion of the .beta. subunit of hCG and a monoclonal antibody directed to a determinant on hCG at a locus sufficiently remote from the carboxy terminal portion of the .beta. subunit of hCG that both antibodies can simultaneously bind to hCG, wherein at least one of the antibodies is delectable when both are bound to hCG.In a presently preferred embodiment, an immunoassay for hCG or hCB.beta. in urine includes a purified, labeled or detectable serum-derived antibody directed to the carboxy-terminal portion of the .beta. subunit of hCG and a matrix-bound monoclonal antibody directed to a locus on the .beta. subunit sufficiently remote from the carboxy-terminal portion that both antibodies can simultaneously bind to hCG or hCG.beta..

摘要翻译： 用于检测和测量样品中的hCG的免疫测定包括针对hCG的β亚单位的羧基末端部分的抗体和针对hCG上的决定簇的单克隆抗体，其位于足够远离β-亚基的β亚基的羧基末端部分 两种抗体可以同时结合hCG的hCG，其中当二者结合hCG时，至少一种抗体是可选的。 在目前优选的实施方案中，尿中hCG或hCBβ的免疫测定包括针对hCG的β亚基的羧基末端部分的纯化的，标记的或可检测的血清衍生的抗体，以及针对基因座的基质结合的单克隆抗体 在足够远离羧基末端部分的β亚基上，两个抗体可以同时结合hCG或hCGβ。