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    Immunoassay for human chorionic gonadotropin
    1.
    发明授权
    Immunoassay for human chorionic gonadotropin 失效
    人绒毛膜促性腺激素免疫测定

    公开(公告)号：US4851356A

    公开(公告)日：1989-07-25

    申请号：US72802

    申请日：1987-07-14

    申请人： Robert E. Canfield ,  Elmo G. Armstrong ,  Paul H. Ehrlich ,  Steven Birken

    发明人： Robert E. Canfield ,  Elmo G. Armstrong ,  Paul H. Ehrlich ,  Steven Birken

    IPC分类号： G01N33/574 ,  G01N33/577 ,  G01N33/76

    CPC分类号： G01N33/76 ,  G01N33/57476 ,  G01N33/577 ,  Y10S436/804 ,  Y10S436/813 ,  Y10S436/814 ,  Y10S436/818 ,  Y10S436/823

    摘要： An immunoassay for detecting and measuring hCG in a sample includes an antibody directed to the carboxy terminal portion of the .beta. subunit of hCG and a monoclonal antibody directed to a determinant on hCG at a locus sufficiently remote from the carboxy terminal portion of the .beta. subunit of hCG that both antibodies can simultaneously bind to hCG, wherein at least one of the antibodies is delectable when both are bound to hCG.In a presently preferred embodiment, an immunoassay for hCG or hCB.beta. in urine includes a purified, labeled or detectable serum-derived antibody directed to the carboxy-terminal portion of the .beta. subunit of hCG and a matrix-bound monoclonal antibody directed to a locus on the .beta. subunit sufficiently remote from the carboxy-terminal portion that both antibodies can simultaneously bind to hCG or hCG.beta..

    摘要翻译： 用于检测和测量样品中的hCG的免疫测定包括针对hCG的β亚单位的羧基末端部分的抗体和针对hCG上的决定簇的单克隆抗体，其位于足够远离β-亚基的β亚基的羧基末端部分 两种抗体可以同时结合hCG的hCG，其中当二者结合hCG时，至少一种抗体是可选的。 在目前优选的实施方案中，尿中hCG或hCBβ的免疫测定包括针对hCG的β亚基的羧基末端部分的纯化的，标记的或可检测的血清衍生的抗体，以及针对基因座的基质结合的单克隆抗体 在足够远离羧基末端部分的β亚基上，两个抗体可以同时结合hCG或hCGβ。

    Antibodies specific for hLH .beta. core fragment
    4.
    发明授权
    Antibodies specific for hLH .beta. core fragment 失效
    针对hLHβ核心片段的抗体

    公开(公告)号：US5976876A

    公开(公告)日：1999-11-02

    申请号：US763669

    申请日：1996-12-11

    申请人： Robert E. Canfield ,  Steven Birken ,  John O'Connor ,  Galina Kovalevskaya

    发明人： Robert E. Canfield ,  Steven Birken ,  John O'Connor ,  Galina Kovalevskaya

    IPC分类号： C07K16/26 ,  C12N5/12

    CPC分类号： C07K16/26

    摘要： This invention provides an antibody which specifically binds to hLH.beta.cf without cross-reacting with hLH, hLH.beta. or hCG.beta.cf. In an embodiment, the monoclonal antibody is designated B505. In a further embodiment, the hybridoma cell line producing the monoclonal antibody B 505 is designated ATCC Accession No.12000. This invention provides different uses of the antibodies. Finally, this invention provides a method for determining the amount of hLH.beta.cf or hLH.beta.cf-related molecule in a sample.

    摘要翻译： 本发明提供了一种特异性结合hLH beta cf而不与hLH，hLHβ或hCGβ比较的交叉反应的抗体。 在一个实施方案中，单克隆抗体命名为B505。 在另一个实施方案中，产生单克隆抗体B 505的杂交瘤细胞系称为ATCC登录号12000。 本发明提供了抗体的不同用途。 最后，本发明提供了一种测定样品中hLH beta cf或hLH beta cf相关分子量的方法。

    Methods of using antibodies against hormone-related determinants
    5.
    发明授权
    Methods of using antibodies against hormone-related determinants 失效
    使用针对激素相关决定簇的抗体的方法

    公开(公告)号：US06339143B1

    公开(公告)日：2002-01-15

    申请号：US08487949

    申请日：1995-06-07

    申请人： Alexander Krichevsky ,  Steven Birken ,  John O'Connor ,  Robert E. Canfield

    发明人： Alexander Krichevsky ,  Steven Birken ,  John O'Connor ,  Robert E. Canfield

    IPC分类号： C07K16/26 ,  G01N33/50 ,  G01N33/569 ,  G01N33/574 ,  G01N33/76

    CPC分类号： C07K16/26 ,  G01N33/5091 ,  G01N33/56966 ,  G01N33/57492 ,  G01N33/76 ,  G01N2333/59 ,  G01N2333/705

    摘要： This invention provides methods for detecting the presence of human malignant cells in a sample of tumor cells; determining whether a tumor present in a human subject is malignant; obtaining an enriched population of live human malignant cells; determining the amount of intact luteinizing hormone in a sample; determining the ovulatory stage of a subject; determining the amount of intact follicle stimulating hormone in a sample; determining the ovulatory stage of a subject; determining the amount of intact human chorionic gonadotropin in a sample; determining whether a subject is pregnant; determining the ovulatory stage of a subject; determining the amount of free &agr; subunit of human luteinizing hormone in a sample; determining whether a subject has a malignant tumor; determining the amount of nicked human chorionic gonadotropin in a sample; and determining the likelihood of a fetus's being afflicted with Down's syndrome.

    摘要翻译： 本发明提供了用于检测肿瘤细胞样品中人恶性细胞存在的方法; 确定存在于人类受试者中的肿瘤是否是恶性的; 获得活的人类恶性细胞的富集群体; 确定样品中完整的黄体生成激素的量; 确定受试者的排卵期; 确定样品中完整的促卵泡激素的量; 确定受试者的排卵期; 确定样品中完整的人绒毛膜促性腺激素的量; 确定受试者是否怀孕; 确定受试者的排卵期; 测定样品中人黄体生成激素的游离α亚基的量; 确定受试者是否具有恶性肿瘤; 确定样品中切口的人绒毛膜促性腺激素的量; 并确定胎儿患有唐氏综合征的可能性。

    Antibodies specific for HLH βcore fragment and uses thereof
    6.
    发明授权
    Antibodies specific for HLH βcore fragment and uses thereof 失效
    对HLH betacore片段特异性的抗体及其用途

    公开(公告)号：US07034124B2

    公开(公告)日：2006-04-25

    申请号：US09404076

    申请日：1999-09-23

    申请人： Robert E. Canfield ,  Steven Birken ,  John O'Connor ,  Galina Kovalevskaya

    发明人： Robert E. Canfield ,  Steven Birken ,  John O'Connor ,  Galina Kovalevskaya

    IPC分类号： C07K16/00

    CPC分类号： C07K16/26

    摘要： This invention provides an antibody which specifically binds to hLHβcf without cross-reacting with hLH, hLHβ or hCGβcf. In an embodiment, the monoclonal antibody is designated B505. In a further embodiment, the hybridoma cell line producing the monoclonal antibody B 505 is designated ATCC Accession No. 12000. This invention provides different uses of the antibodies. Finally, this invention provides a method for determining the amount of hLHβcf or hLHβcf-related molecule in a sample.

    摘要翻译： 本发明提供了与hLHbetacf特异性结合而不与hLH，hLHbeta或hCGbetacf交叉反应的抗体。 在一个实施方案中，单克隆抗体命名为B505。 在另一个实施方案中，产生单克隆抗体B 505的杂交瘤细胞系被命名为ATCC登录号12000.本发明提供了抗体的不同用途。 最后，本发明提供了一种测定样品中hLHbetacf或hLHbetacf相关分子量的方法。

    Sensitive bioassay using monoclonal antibodies which bind to hormone-receptor complexes
    7.
    发明授权
    Sensitive bioassay using monoclonal antibodies which bind to hormone-receptor complexes 失效
    使用与激素受体复合物结合的单克隆抗体的敏感生物测定

    公开(公告)号：US5284778A

    公开(公告)日：1994-02-08

    申请号：US759645

    申请日：1991-09-12

    申请人： Robert E. Canfield ,  E. Glenn Armstrong ,  William R. Moyle ,  Gordon J. MacDonald ,  Donna M. Anderson

    发明人： Robert E. Canfield ,  E. Glenn Armstrong ,  William R. Moyle ,  Gordon J. MacDonald ,  Donna M. Anderson

    IPC分类号： G01N33/76 ,  G01N33/536

    CPC分类号： G01N33/76

    摘要： This invention provides a quantitative assay for determining the amount of a biologically active ligand selected from the group consisting of human chorionic gonadotropin and luetinizing hormone present in a sample comprising contacting the sample with both the receptor to which the ligand naturally binds in order to effect its biologicaly activity and a monoclonal antibody directed to the ligand or to a complex of the ligand and the receptor so as to form a complex of the ligand bound to both the receptor, at the site to which the ligand naturally binds to the receptor, and the monoclonal antibody. In the complex so formed, either the receptor or the monoclonal antibody is labeled with a detectable marker and a determination is made of the amount of labeled receptor or of labeled monoclonal antibody bound to the ligand or the amount of labeled receptor or of labeled monoclonal antibody not bound to the ligand, or both such amounts.This invention further provides a quantitative assay for determining the amount present in a sample of a receptor to which a biologically acitve ligand selected from the group consisting of human chorionic gonadotropin and luetinizing hormone naturally binds in order to effect its biological activity. Finally, this invention provides kits for quantitatively assaying for the amount present in a sample of a biologically active ligand or for a receptor to which the ligand naturally binds in order to effect its biological activity.

    摘要翻译： 本发明提供了用于确定选自样品中存在的人绒毛膜促性腺激素和呼吸系数激素的生物活性配体的量的定量测定法，其包括将样品与配体天然结合的受体相接触以实现其 生物活性和针对配体或配体和受体的复合物的单克隆抗体，以便在配体天然与受体结合的位点处形成与受体结合的配体的复合物，以及 单克隆抗体。 在如此形成的复合物中，受体或单克隆抗体用可检测的标记物标记，并且确定与配体结合的标记受体或标记的单克隆抗体的量或标记的受体或标记的单克隆抗体的量 不与配体结合，或两者都相同。 本发明进一步提供了一种定量测定法，用于确定存在于受体的样品中的量，其中选自人绒毛膜促性腺激素和呼吸系数激素的生物活性配体天然结合以实现其生物学活性。 最后，本发明提供了用于定量测定生物活性配体样品中存在的量或配体天然结合的受体以实现其生物学活性的试剂盒。