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1.发明申请Methods and compositions for high throughput identification of protein/nucleic acid binding pairs 失效高通量鉴定蛋白质/核酸结合对的方法和组合物公开(公告)号：US20050003395A1
公开(公告)日：2005-01-06
申请号：US10813639
申请日：2004-03-29
申请人： Robert Gellibolian , Eric Leproust
发明人： Robert Gellibolian , Eric Leproust
CPC分类号： C12Q1/6837 , B01J2219/00378 , B01J2219/00387 , B01J2219/00497 , B01J2219/00527 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00677 , B01J2219/00707 , B01J2219/00722 , B01J2219/00729 , C12Q1/6818 , C40B40/06 , C40B60/14 , C12Q2565/531 , C12Q2561/101
摘要： Methods and compositions for high-throughput identification of protein/nucleic acid binding pairs are provided. In the subject methods, a nucleic acid probe array, e.g., a molecular beacon probe array, is contacted with a target nucleic acid population to produce a hybridized array. The resultant hybridized array is then contacted with a population of proteins to produce a protein bound array. Any resultant array surface bound target nucleic acid/protein complexes are then detected to identify protein/nucleic acid binding pairs. In certain embodiments, the protein and/or nucleic acid members of the identified protein/nucleic acid binding pairs are further characterized. Also provided are systems and kits for use in practicing the subject methods. The subject invention finds use in a variety of different applications.
摘要翻译： 提供了用于蛋白质/核酸结合对的高通量鉴定的方法和组合物。 在本主题方法中，将核酸探针阵列（例如分子信标探针阵列）与靶核酸群体接触以产生杂交阵列。 然后将所得杂交阵列与蛋白质群体接触以产生蛋白质结合阵列。 然后检测任何得到的阵列表面结合的靶核酸/蛋白质复合物，以鉴定蛋白质/核酸结合对。 在某些实施方案中，进一步表征鉴定的蛋白质/核酸结合对的蛋白质和/或核酸成员。 还提供了用于实践主题方法的系统和工具包。 本发明可用于各种不同的应用。
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2.发明授权Methods and compositions for high throughput identification of protein/nucleic acid binding pairs 失效高通量鉴定蛋白质/核酸结合对的方法和组合物公开(公告)号：US06713262B2
公开(公告)日：2004-03-30
申请号：US10180437
申请日：2002-06-25
IPC分类号： C07H2104
CPC分类号： C12Q1/6837 , B01J2219/00378 , B01J2219/00387 , B01J2219/00497 , B01J2219/00527 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00677 , B01J2219/00707 , B01J2219/00722 , B01J2219/00729 , C12Q1/6818 , C40B40/06 , C40B60/14 , C12Q2565/531 , C12Q2561/101
摘要： Methods and compositions for high-throughput identification of protein/nucleic acid binding pairs are provided. In the subject methods, a nucleic acid probe array, e.g., a molecular beacon probe array, is contacted with a target nucleic acid population to produce a hybridized array. The resultant hybridized array is then contacted with a population of proteins to produce a protein bound array. Any resultant array surface bound target nucleic acid/protein complexes are then detected to identify protein/nucleic acid binding pairs. In certain embodiments, the protein and/or nucleic acid members of the identified protein/nucleic acid binding pairs are further characterized. Also provided are systems and kits for use in practicing the subject methods. The subject invention finds use in a variety of different applications.
摘要翻译： 提供了用于蛋白质/核酸结合对的高通量鉴定的方法和组合物。 在本主题方法中，将核酸探针阵列（例如分子信标探针阵列）与靶核酸群体接触以产生杂交阵列。 然后将所得杂交阵列与蛋白质群体接触以产生蛋白质结合阵列。 然后检测任何得到的阵列表面结合的靶核酸/蛋白质复合物，以鉴定蛋白质/核酸结合对。 在某些实施方案中，进一步表征鉴定的蛋白质/核酸结合对的蛋白质和/或核酸成员。 还提供了用于实践主题方法的系统和工具包。 本发明可用于各种不同的应用。
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公开(公告)号：US20070264667A1
公开(公告)日：2007-11-15
申请号：US10934647
申请日：2004-09-03
申请人： Robert Gellibolian , Riaz Rouhani
发明人： Robert Gellibolian , Riaz Rouhani
IPC分类号： G01N33/53
CPC分类号： C12Q1/48
摘要： Embodiments of the present invention are directed to sensitive, specific, and commercially feasible assays for transferase activity. Various embodiments of the present invention include artificial, multifunctional substrates specific for particular transferases that are chemically altered by the transferases to produce easily detectable, modified, multifunctional substrates. In one class of embodiments, the artificial, multifunctional substrate comprises a small-molecule-substrate component, or small-molecule-substrate-analog component, linked by a linking component to a biopolymer-substrate-mimetic or biopolymer-substrate-analog component. At least two, generally well-separated reporter moieties are included in the artificial, multifunctional substrate. The transferase, for which the artificial, multifunctional substrate is designed to serve as an assay reagent, catalyzes a generally covalent modification of the artificial, multifunctional substrate to produce a modified, artificial, multifunctional substrate reaction product in which the two reporter moieties are closely positioned to one another. When closely positioned to one another, the reporter moieties are detectable by one of various instrumental techniques.
摘要翻译： 本发明的实施方案涉及用于转移酶活性的敏感的，特异性的和商业可行的测定。 本发明的各种实施方案包括对特定转移酶特异的人造的多功能底物，其通过转移酶进行化学改变以产生易于检测的，修饰的多功能底物。 在一类实施方案中，人造多功能底物包括通过连接组分与生物聚合物 - 底物 - 模拟物或生物聚合物 - 底物 - 类似物组分连接的小分子底物组分或小分子底物 - 类似物组分。 在人造多功能底物中包含至少两个通常分离得很好的报道分子。 将人造多功能底物设计用作测定试剂的转移酶催化人造多功能底物的通常共价修饰，以产生修饰的人造多功能底物反应产物，其中两个报道部分紧密定位 彼此。 当彼此紧密定位时，报道分子可通过各种仪器技术之一检测。
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4.发明授权Methods and compositions for high throughput identification of protein/nucleic acid binding pairs 失效高通量鉴定蛋白质/核酸结合对的方法和组合物公开(公告)号：US07192710B2
公开(公告)日：2007-03-20
申请号：US10813639
申请日：2004-03-29
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , B01J2219/00378 , B01J2219/00387 , B01J2219/00497 , B01J2219/00527 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00677 , B01J2219/00707 , B01J2219/00722 , B01J2219/00729 , C12Q1/6818 , C40B40/06 , C40B60/14 , C12Q2565/531 , C12Q2561/101
摘要： Methods and compositions for high-throughput identification of protein/nucleic acid binding pairs are provided. In the subject methods, a nucleic acid probe array, e.g., a molecular beacon probe array, is contacted with a target nucleic acid population to produce a hybridized array. The resultant hybridized array is then contacted with a population of proteins to produce a protein bound array. Any resultant array surface bound target nucleic acid/protein complexes are then detected to identify protein/nucleic acid binding pairs. In certain embodiments, the protein and/or nucleic acid members of the identified protein/nucleic acid binding pairs are further characterized. Also provided are systems and kits for use in practicing the subject methods. The subject invention finds use in a variety of different applications.
摘要翻译： 提供了用于蛋白质/核酸结合对的高通量鉴定的方法和组合物。 在本主题方法中，将核酸探针阵列（例如分子信标探针阵列）与靶核酸群体接触以产生杂交阵列。 然后将所得杂交阵列与蛋白质群体接触以产生蛋白质结合阵列。 然后检测任何得到的阵列表面结合的靶核酸/蛋白质复合物，以鉴定蛋白质/核酸结合对。 在某些实施方案中，进一步表征鉴定的蛋白质/核酸结合对的蛋白质和/或核酸成员。 还提供了用于实践主题方法的系统和工具包。 本发明可用于各种不同的应用。
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