公开(公告)号：US20050123555A1

公开(公告)日：2005-06-09

申请号：US10929234

申请日：2004-08-30

申请人： Robert Olmsted ,  Paula Keith ,  Sergey Dryga ,  Ian Caley ,  Maureen Maughan ,  Robert Johnston ,  Nancy Davis ,  Ronald Swanstrom

发明人： Robert Olmsted ,  Paula Keith ,  Sergey Dryga ,  Ian Caley ,  Maureen Maughan ,  Robert Johnston ,  Nancy Davis ,  Ronald Swanstrom

IPC分类号： A61K39/00 ,  A61K39/21 ,  C07K14/16 ,  C07K14/18 ,  C12N7/02 ,  C12Q1/68 ,  A61K39/29 ,  C07H21/02 ,  C12N7/00 ,  C12N15/86

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K39/12 ,  A61K39/21 ,  A61K2039/5256 ,  A61K2039/5258 ,  A61K2039/54 ,  A61K2039/545 ,  A61K2039/575 ,  C12N7/00 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2740/16222 ,  C12N2740/16234 ,  C12N2770/36143 ,  C12N2770/36151

摘要： The present invention provides methods and compositions comprising a population of alphavirus replicon particles comprising two or more isolated nucleic acids selected from 1) an isolated nucleic acid encoding an env gene product or an immunogenic fragment thereof of a human immunodeficiency virus, 2) an isolated nucleic acid encoding a gag gene product or an immunogenic fragment thereof of a human immunodeficiency virus, wherein the gag gene product or immunogenic fragment thereof is modified to inhibit formation of virus-like particles containing the gag gene product or the immunogenic fragment thereof and their release from a cell, and 3) an isolated nucleic acid encoding a pol gene product or an immunogenic fragment thereof of a human immunodeficiency virus, wherein the pol gene product or immunogenic fragment thereof is modified to inhibit protease, integrase, RNase H and/or reverse transcriptase activity, and wherein the nucleic acids are each contained within a separate alphavirus replicon particle.

摘要翻译： 本发明提供方法和组合物，其包含包含两种或多种分离的核酸的甲型病毒复制子颗粒群，所述分离的核酸选自1）编码人免疫缺陷病毒的env基因产物或其免疫原性片段的分离的核酸，2）分离的核酸 酸编码人免疫缺陷病毒的gag基因产物或其免疫原性片段，其中修饰gag基因产物或其免疫原性片段以抑制含有gag基因产物或其免疫原性片段的病毒样颗粒的形成，以及它们从 细胞，以及3）编码人免疫缺陷病毒的pol基因产物或其免疫原性片段的分离的核酸，其中pol基因产物或其免疫原性片段被修饰以抑制蛋白酶，整合酶，RNA酶H和/或逆转录酶 活性，并且其中所述核酸各自包含在单独的阿夫法韦内 我们复制品颗粒。

公开(公告)号：US06783939B2

公开(公告)日：2004-08-31

申请号：US09991258

申请日：2001-11-16

申请人： Robert Olmsted ,  Paula Keith ,  Sergey Dryga ,  Ian Caley ,  Maureen Maughan ,  Robert Johnston ,  Nancy Davis ,  Ronald Swanstrom

发明人： Robert Olmsted ,  Paula Keith ,  Sergey Dryga ,  Ian Caley ,  Maureen Maughan ,  Robert Johnston ,  Nancy Davis ,  Ronald Swanstrom

IPC分类号： C12Q168

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K39/12 ,  A61K39/21 ,  A61K2039/5256 ,  A61K2039/5258 ,  A61K2039/54 ,  A61K2039/545 ,  A61K2039/575 ,  C12N7/00 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2740/16222 ,  C12N2740/16234 ,  C12N2770/36143 ,  C12N2770/36151

摘要： The present invention provides methods and compositions comprising a population of alphavirus replicon particles comprising two or more isolated nucleic acids selected from 1) an isolated nucleic acid encoding an env gene product or an immunogenic fragment thereof of a human immunodeficiency virus, 2) an isolated nucleic acid encoding a g&agr;g gene product or an immunogenic fragment thereof of a human immunodeficiency virus, wherein the g&agr;g gene product or immunogenic fragment thereof is modified to inhibit formation of virus-like particles containing the g&agr;g gene product or the immunogenic fragment thereof and their release from a cell, and 3) an isolated nucleic acid encoding a pol gene product or an immunogenic fragment thereof of a human immunodeficiency virus, wherein the pol gene product or immunogenic fragment thereof is modified to inhibit protease, integrase, RNase H and/or reverse transcriptase activity, and wherein the nucleic acids are each contained within a separate alphavirus replicon particle.

摘要翻译： 本发明提供方法和组合物，其包含包含两种或多种分离的核酸的甲型病毒复制子颗粒群，所述分离的核酸选自1）编码人免疫缺陷病毒的env基因产物或其免疫原性片段的分离的核酸，2）分离的核酸 编码人免疫缺陷病毒的galphag基因产物或其免疫原性片段的酸，其中所述galphag基因产物或其免疫原性片段被修饰以抑制含有galphag基因产物或其免疫原性片段的病毒样颗粒的形成及其从 细胞，以及3）编码人免疫缺陷病毒的pol基因产物或其免疫原性片段的分离的核酸，其中pol基因产物或其免疫原性片段被修饰以抑制蛋白酶，整合酶，RNA酶H和/或逆转录酶 活性，并且其中所述核酸各自包含在分离物内 食用甲病毒复制子颗粒。