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    Alphavirus vectors and virosomes with modified HIV genes for use in vaccines
    2.
    发明授权
    Alphavirus vectors and virosomes with modified HIV genes for use in vaccines 有权
    甲病毒载体和具有修饰的HIV基因的病毒体用于疫苗

    公开(公告)号：US06783939B2

    公开(公告)日：2004-08-31

    申请号：US09991258

    申请日：2001-11-16

    申请人： Robert Olmsted ,  Paula Keith ,  Sergey Dryga ,  Ian Caley ,  Maureen Maughan ,  Robert Johnston ,  Nancy Davis ,  Ronald Swanstrom

    发明人： Robert Olmsted ,  Paula Keith ,  Sergey Dryga ,  Ian Caley ,  Maureen Maughan ,  Robert Johnston ,  Nancy Davis ,  Ronald Swanstrom

    IPC分类号： C12Q168

    CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K39/12 ,  A61K39/21 ,  A61K2039/5256 ,  A61K2039/5258 ,  A61K2039/54 ,  A61K2039/545 ,  A61K2039/575 ,  C12N7/00 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2740/16222 ,  C12N2740/16234 ,  C12N2770/36143 ,  C12N2770/36151

    摘要： The present invention provides methods and compositions comprising a population of alphavirus replicon particles comprising two or more isolated nucleic acids selected from 1) an isolated nucleic acid encoding an env gene product or an immunogenic fragment thereof of a human immunodeficiency virus, 2) an isolated nucleic acid encoding a g&agr;g gene product or an immunogenic fragment thereof of a human immunodeficiency virus, wherein the g&agr;g gene product or immunogenic fragment thereof is modified to inhibit formation of virus-like particles containing the g&agr;g gene product or the immunogenic fragment thereof and their release from a cell, and 3) an isolated nucleic acid encoding a pol gene product or an immunogenic fragment thereof of a human immunodeficiency virus, wherein the pol gene product or immunogenic fragment thereof is modified to inhibit protease, integrase, RNase H and/or reverse transcriptase activity, and wherein the nucleic acids are each contained within a separate alphavirus replicon particle.

    摘要翻译： 本发明提供方法和组合物，其包含包含两种或多种分离的核酸的甲型病毒复制子颗粒群，所述分离的核酸选自1）编码人免疫缺陷病毒的env基因产物或其免疫原性片段的分离的核酸，2）分离的核酸 编码人免疫缺陷病毒的galphag基因产物或其免疫原性片段的酸，其中所述galphag基因产物或其免疫原性片段被修饰以抑制含有galphag基因产物或其免疫原性片段的病毒样颗粒的形成及其从 细胞，以及3）编码人免疫缺陷病毒的pol基因产物或其免疫原性片段的分离的核酸，其中pol基因产物或其免疫原性片段被修饰以抑制蛋白酶，整合酶，RNA酶H和/或逆转录酶 活性，并且其中所述核酸各自包含在分离物内 食用甲病毒复制子颗粒。

    Nucleic acids encoding modified South African HIV-1 subtype C gag proteins
    5.
    发明授权
    Nucleic acids encoding modified South African HIV-1 subtype C gag proteins 失效
    编码修饰的南非HIV-1亚型C gag蛋白的核酸

    公开(公告)号：US07910716B2

    公开(公告)日：2011-03-22

    申请号：US12357099

    申请日：2009-01-21

    申请人： Robert Edward Johnston ,  Salim Abdol Karim ,  Lynn Morris ,  Ronald Swanstrom ,  Carolyn Williamson

    发明人： Robert Edward Johnston ,  Salim Abdol Karim ,  Lynn Morris ,  Ronald Swanstrom ,  Carolyn Williamson

    IPC分类号： C07H21/04 ,  A61K39/21

    CPC分类号： A61K39/21 ,  A61K39/00 ,  A61K39/12 ,  A61K2039/5256 ,  C07K14/005 ,  C12N7/00 ,  C12N2740/16051 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2740/16222 ,  C12N2740/16234

    摘要： Embodiments of the invention provide processes for the selection of HIV-1 subtype (clade) C isolates, selected HIV-1 subtype C isolates, their genes and modifications and derivatives thereof for use in prophylactic and therapeutic vaccines to produce proteins and polypeptides for the purpose of eliciting protection against HIV infection or disease. A process for the selection of HIV subtype isolates comprises the steps of isolating viruses from recently infected subjects; generating a consensus sequence for at least part of at least one HIV gene by identifying the most common codon or amino acid among the isolated viruses; and selecting the isolated virus or viruses with a high sequence identity to the consensus sequence. HIV-1 subtype C isolates, designated Du422, Du 151 and Du 179 (assigned Accession Numbers 01032114, 00072724 and 00072725, respectively, by the European Collection of Cell Cultures) are also provided.

    摘要翻译： 本发明的实施方案提供用于选择HIV-1亚型（进化枝）C分离株，选择的HIV-1亚型C分离物，其基因及其修饰和衍生物的方法，用于预防和治疗疫苗以产生用于该目的的蛋白质和多肽 引起艾滋病毒感染或疾病的保护。 选择HIV亚型分离物的方法包括从最近感染的受试者中分离病毒的步骤; 通过鉴定分离的病毒中最常见的密码子或氨基酸，产生至少一部分HIV基因的共有序列; 并选择与共有序列具有高序列同一性的分离的病毒或病毒。 还提供了称为Du422，Du 151和Du 179（分别由欧洲细胞培养物集合分配的登录号01032114，00072724和00072725）的HIV-1亚型C分离物。