公开(公告)号：US07005274B1

公开(公告)日：2006-02-28

申请号：US09661848

申请日：2000-09-14

申请人： Robert Terkeltaub ,  Anne N. Murphy ,  James A. Dykens ,  Soumitra S. Ghosh ,  Robert E. Davis ,  Andrew E. Granston, Jr.

发明人： Robert Terkeltaub ,  Anne N. Murphy ,  James A. Dykens ,  Soumitra S. Ghosh ,  Robert E. Davis ,  Andrew E. Granston, Jr.

IPC分类号： C12Q1/02

CPC分类号： G01N33/5088 ,  G01N33/6887 ,  G01N2333/4722 ,  G01N2333/495 ,  G01N2333/78 ,  G01N2400/40 ,  G01N2800/102 ,  G01N2800/105

摘要： The present invention relates to improved diagnostic methods for early detection of a risk for developing an arthritic disorder in humans, and screening assays for therapeutic agents useful in the treatment of arthritic disorders, by comparing the levels of one or more indicators of altered mitochondrial function. Indicators of altered mitochondrial function include enzymes such as mitochondrial enzymes and ATP biosynthesis factors. Other indicators of altered mitochondrial function include mitochondrial mass, mitochondrial number and mitochondrial DNA content, cellular responses to elevated intracellular calcium and to apoptogens, and free radical production. Methods of treating, and of stratifying, human patients as such methods relate to disclosed indicators of altered mitchondrial function are also provided.

摘要翻译： 本发明涉及通过比较改变的线粒体功能的一个或多个指示剂的水平，用于早期发现人类关节炎疾病的风险的改进的诊断方法以及用于治疗关节炎疾病的治疗剂的筛选测定。 改变线粒体功能的指标包括线粒体酶和ATP生物合成因子等酶。 改变的线粒体功能的其他指标包括线粒体质量，线粒体数量和线粒体DNA含量，细胞内升高的钙和凋亡细胞的反应以及自由基生成。 还提供了用于治疗和分层人类患者的方法，这些方法涉及公开的改变的脑膜功能指标。

公开(公告)号：US06562563B1

公开(公告)日：2003-05-13

申请号：US09434354

申请日：1999-11-03

申请人： Anne N. Murphy ,  William Clevenger ,  Sandra Eileen Wiley ,  Alexander Y. Andreyev ,  Luciano G. Frigeri ,  Gonul Velecelebi ,  Robert E. Davis

发明人： Anne N. Murphy ,  William Clevenger ,  Sandra Eileen Wiley ,  Alexander Y. Andreyev ,  Luciano G. Frigeri ,  Gonul Velecelebi ,  Robert E. Davis

IPC分类号： C12N500

CPC分类号： G01N33/5079 ,  C07K14/43595 ,  C07K2319/00 ,  C07K2319/21 ,  C07K2319/23 ,  C07K2319/50 ,  C07K2319/60 ,  C12N9/90 ,  C12N15/62 ,  G01N33/542

摘要： Compositions and methods are provided for identifying agents that alter mitochondrial membrane permeability transition. The screening methods generally detect agents that alter the interaction between the mitochondrial adenine nucleotide translocator and cyclophilin D. Such agents may be used, for example, in the treatment of a variety of conditions associated with altered mitochondrial function.

摘要翻译： 提供组合物和方法用于鉴定改变线粒体膜通透性转换的药剂。 筛选方法通常检测改变线粒体腺嘌呤核苷酸转运体和亲环蛋白D之间的相互作用的试剂。这种试剂可用于例如治疗与线粒体功能改变相关的多种病症。

公开(公告)号：US06797467B1

公开(公告)日：2004-09-28

申请号：US09709785

申请日：2000-11-03

申请人： Anne N. Murphy ,  William Clevenger ,  Sandra Eileen Wiley ,  Alexander Y. Andreyev ,  Luciano G. Frigeri ,  Gonul Velicelebi ,  Robert E. Davis

发明人： Anne N. Murphy ,  William Clevenger ,  Sandra Eileen Wiley ,  Alexander Y. Andreyev ,  Luciano G. Frigeri ,  Gonul Velicelebi ,  Robert E. Davis

IPC分类号： C12Q168

CPC分类号： G01N33/5079 ,  C07K14/43595 ,  C07K2319/00 ,  C07K2319/21 ,  C07K2319/23 ,  C07K2319/50 ,  C07K2319/60 ,  C12N9/90 ,  C12N15/62 ,  G01N33/542

摘要： Compositions and methods are provided for identifying agents that alter mitochondrial membrane permeability transition. The screening methods generally detect agents that alter the interaction between the mitochondrial adenine nucleotide translocator and cyclophilin D. Such agents may be used, for example, in the treatment of a variety of conditions associated with altered mitochondrial function.

公开(公告)号：US06808873B2

公开(公告)日：2004-10-26

申请号：US09765104

申请日：2001-01-16

申请人： Anne N. Murphy ,  Amy K. Stout

发明人： Anne N. Murphy ,  Amy K. Stout

IPC分类号： C12Q100

CPC分类号： G01N33/5079

摘要： The invention provides methods for screening for agents that modulate mitochondrial function and in particular mitochondrial regulation of intracellular calcium. The methods may be used to detect agents that bind to a mitochondrial calcium uniporter and may also detect inhibitors or uncouplers of mitochondrial respiration. Agents identified using the screens provided herein have application in the prevention and treatment of a variety of diseases associated with abnormal mitochondrial function.

摘要翻译： 本发明提供筛选调节线粒体功能，特别是细胞内钙线粒体调节剂的方法。 该方法可用于检测结合线粒体钙单转运体的试剂，并且还可以检测线粒体呼吸的抑制剂或解偶联剂。 使用本文提供的筛选鉴定的药剂可用于预防和治疗与异常线粒体功能相关的各种疾病。