公开(公告)号：US20230221311A1

公开(公告)日：2023-07-13

申请号：US18188549

申请日：2023-03-23

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Antje Baeumner ,  Franziska Beck ,  Carina Horn

IPC: G01N33/543 ,  G01N33/553 ,  B01L3/00 ,  G01N27/327 ,  G01N27/48

CPC classification number: G01N33/5438 ,  G01N33/553 ,  B01L3/502761 ,  G01N27/3277 ,  G01N27/3278 ,  G01N27/48 ,  B01L2300/0645 ,  B01L2200/0647

Abstract: A method for detecting at least one analyte by electrochemical detection, a working electrode of an analyte sensor and an analyte sensor for detecting at least one analyte in a sample by electrochemical detection. The method comprises contacting a fluid sample suspected to comprise the at least one analyte with the surface of an electrode comprising a binding agent capable of binding to the analyte; contacting the fluid sample with a detection agent comprising a further binding agent capable of binding to the analyte and a label, the label comprising a metal nanoparticle with a standard redox potential E° between 0 V and 1.2 V forming a detection complex on the surface of the electrode comprising the binding agent, the detection agent and the analyte precipitating at least a part of the label onto the electrode surface; and detecting the analyte by electrochemical detection.