公开(公告)号：US20200041502A1

公开(公告)日：2020-02-06

申请号：US16599480

申请日：2019-10-11

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Jens Christian Bolle ,  Martin Eduardo Silvestre ,  Stephan Hug ,  Carolyn Bylda

IPC: G01N33/543 ,  G01N33/553 ,  B01J13/14 ,  B03C1/01

Abstract: The present disclosure relates to magnetic particles, wherein each particle has a polymer matrix and at least one magnetic core (M), wherein the polymer matrix has at least one crosslinked polymer and wherein the magnetic particle has a particle size in the range of from 1 to 60 micrometer. Further, the present disclosure relates to a method of preparing such particles, and to particles obtainable or obtained by said method. Moreover, the present disclosure uses these magnetic particles for qualitative and/or quantitative determination of at least one analyte in a fluid. Further, the present disclosure relates to a method for determining at least one analyte in a fluid sample using the contacting of a magnetic particle of the disclosure or a magnetic particle obtained by the method of the present disclosure with a fluid sample having or suspected to have the at least one analyte.

公开(公告)号：US10573443B2

公开(公告)日：2020-02-25

申请号：US15170251

申请日：2016-06-01

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Christoph Seidel ,  Jens Christian Bolle ,  Sandra Recklies

IPC: H01F1/44 ,  C08F257/00 ,  C08K9/10

Abstract: Disclosed is a simplified process for producing magnetic polymer particles. The process comprises: (a) providing a composition having a liquid monomer which is radical polymerizable, a radical initiator soluble in the monomer, a steric stabilizer, and a ferrofluid comprising surfactant-coated colloidal magnetic particles in a carrier fluid which is miscible with the monomer; (b) preparing an emulsion from a polar solvent which is immiscible with the monomer, and the composition of step (a); (c) adding seed polymer particles to the emulsion, mixing to form a seeded emulsion, and incubating the seeded emulsion, thereby swelling the seed polymer particles; and (d) activating the radical initiator and polymerizing the monomer in the swollen seed polymer particles; thereby producing the magnetic polymer particles. The process forms monodisperse magnetic particles. The particles are characterized by a uniform distribution of magnetic material, and an absence of magnetite bleeding.

公开(公告)号：US20160276080A1

公开(公告)日：2016-09-22

申请号：US15170251

申请日：2016-06-01

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Christoph Seidel ,  Jens Christian Bolle ,  Sandra Recklies

IPC: H01F1/44 ,  C08F257/00 ,  C08K9/10

CPC classification number: H01F1/44 ,  C08F257/00 ,  C08K9/10 ,  C08K2201/01 ,  H01F1/0054 ,  C08F212/36 ,  C08F212/08 ,  C08F2/44

Abstract: Disclosed is a simplified process for producing magnetic polymer particles. The process comprises: (a) providing a composition having a liquid monomer which is radical polymerizable, a radical initiator soluble in the monomer, a steric stabilizer, and a ferrofluid comprising surfactant-coated colloidal magnetic particles in a carrier fluid which is miscible with the monomer; (b) preparing an emulsion from a polar solvent which is immiscible with the monomer, and the composition of step (a); (c) adding seed polymer particles to the emulsion, mixing to form a seeded emulsion, and incubating the seeded emulsion, thereby swelling the seed polymer particles; and (d) activating the radical initiator and polymerizing the monomer in the swollen seed polymer particles; thereby producing the magnetic polymer particles. The process forms monodisperse magnetic particles. The particles are characterized by a uniform distribution of magnetic material, and an absence of magnetite bleeding.

Abstract translation: 公开了用于制备磁性聚合物颗粒的简化方法。 该方法包括：（a）提供具有可自由基聚合的液体单体的组合物，可溶于单体的自由基引发剂，空间稳定剂和包含表面活性剂涂覆的胶体磁性颗粒在载体流体中的铁磁流体，该载体流体可与 单体; （b）从与单体不混溶的极性溶剂和步骤（a）的组成制备乳液; （c）将种子聚合物颗粒加入到乳液中，混合形成接种的乳液，并孵育接种的乳液，从而使种子聚合物颗粒溶胀; 和（d）活化自由基引发剂并使单体在溶胀的种子聚合物颗粒中聚合; 从而产生磁性聚合物颗粒。 该过程形成单分散磁性颗粒。 颗粒的特征在于磁性材料的分布均匀，并且不存在磁铁矿渗出。