公开(公告)号：US20180346886A1

公开(公告)日：2018-12-06

申请号：US16059096

申请日：2018-08-09

Applicant: Roche Diagnostics Operations, Inc. ,  Roche Diabetes Care, Inc.

Inventor： Daniela Beck ,  Mara Boenitz-Dulat ,  Peter Kratzsch ,  Thomas Streidl ,  Carina Horn

IPC: C12N9/04 ,  C12Q1/32 ,  C12P7/18

Abstract: The present invention relates to a mutant 3-hydroxybutyrate dehydrogenase (3-HBDH) with improved performance relative to the wild-type 3-HBDH, a nucleic acid encoding the mutant 3-HBDH, a cell comprising the mutant 3-HBDH or the nucleic acid, a method of determining the amount or concentration of 3-hydroxybutyrate in a sample, and a device for determining the amount or concentration of 3-hydroxybutyrate in a sample.

公开(公告)号：US10508267B2

公开(公告)日：2019-12-17

申请号：US16014355

申请日：2018-06-21

Applicant: Roche Diagnostics Operations, Inc. ,  Roche Diabetes Care, Inc.

Inventor： Peter Kratzsch ,  Mara Boenitz-Dulat ,  Daniela Beck ,  Thomas Streidl ,  Stacy Hunt DuVall

IPC: C12N9/04 ,  C12Q1/54 ,  C12Q1/32

Abstract: The present disclosure relates to a mutant 3-hydroxybutyrate dehydrogenase (3-HBDH) with improved performance relative to the wild-type 3-HBDH, a nucleic acid encoding the mutant 3-HBDH, a cell having the mutant 3-HBDH or the nucleic acid, and/or a method of determining the amount or concentration of 3-hydroxybutyrate in a sample. Also disclosed is the use of the mutant 3-HBDH for determining the amount or concentration of 3-hydroxybutyrate in a sample, and a device for determining the amount or concentration of 3-hydroxybutyrate in a sample.

公开(公告)号：US20180291354A1

公开(公告)日：2018-10-11

申请号：US16014355

申请日：2018-06-21

Applicant: Roche Diagnostics Operations, Inc. ,  Roche Diabetes Care, Inc.

Inventor： Peter Kratzsch ,  Mara Boenitz-Dulat ,  Daniela Beck ,  Thomas Streidl ,  Stacy Hunt DuVall

IPC: C12N9/04 ,  C12Q1/32 ,  C12Q1/54

CPC classification number: C12N9/0006 ,  C12Q1/32 ,  C12Q1/54 ,  C12Y101/0103

Abstract: The present disclosure relates to a mutant 3-hydroxybutyrate dehydrogenase (3-HBDH) with improved performance relative to the wild-type 3-HBDH, a nucleic acid encoding the mutant 3-HBDH, a cell having the mutant 3-HBDH or the nucleic acid, and/or a method of determining the amount or concentration of 3-hydroxybutyrate in a sample. Also disclosed is the use of the mutant 3-HBDH for determining the amount or concentration of 3-hydroxybutyrate in a sample, and a device for determining the amount or concentration of 3-hydroxybutyrate in a sample.

公开(公告)号：US10704029B2

公开(公告)日：2020-07-07

申请号：US16059096

申请日：2018-08-09

Applicant: Roche Diagnostics Operations, Inc. ,  Roche Diabetes Care, Inc.

Inventor： Daniela Beck ,  Mara Boenitz-Dulat ,  Peter Kratzsch ,  Thomas Streidl ,  Carina Horn

IPC: C12N9/02 ,  C12N9/04 ,  C12Q1/32 ,  C12P7/18

Abstract: The present invention relates to a mutant 3-hydroxybutyrate dehydrogenase (3-HBDH) with improved performance relative to the wild-type 3-HBDH, a nucleic acid encoding the mutant 3-HBDH, a cell comprising the mutant 3-HBDH or the nucleic acid, a method of determining the amount or concentration of 3-hydroxybutyrate in a sample, and a device for determining the amount or concentration of 3-hydroxybutyrate in a sample.

公开(公告)号：US20140212903A1

公开(公告)日：2014-07-31

申请号：US14226884

申请日：2014-03-27

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Stacy H. Duvall ,  Peter Gebauer ,  Dieter Heindl ,  Carina Horn ,  Peter Kratzsch ,  Michael Marquant ,  Thomas Meier

IPC: C12Q1/54

CPC classification number: C12Q1/54 ,  C07C281/20 ,  C07D295/215

Abstract: Azo compounds are disclosed that do not form azoxy dimers and that do not have any reactive nitroso groups. Also disclosed are use of the azo compounds as mediators in optical and electrochemical diagnostic methods, as well as detection reagents, kits and test elements that include such azo compounds and that can be used in the diagnostic methods.

Abstract translation: 公开了不形成氮氧化二聚体并且不具有任何反应性亚硝基的偶氮化合物。 还公开了在光学和电化学诊断方法中使用偶氮化合物作为介体，以及包括这种偶氮化合物并且可用于诊断方法的检测试剂，试剂盒和测试元件。