公开(公告)号：US20210355472A1

公开(公告)日：2021-11-18

申请号：US17386106

申请日：2021-07-27

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Thomas Greiner-Stoeffele ,  Stefan Schoenert

IPC: C12N9/52 ,  C07K14/195 ,  C12N5/071

Abstract: The present disclosure provides the sequence of a Paenibacillus polymyxa preproenzyme which is the precursor of a neutral protease, expression thereof in a transformed host organism, and methods for production of the neutral protease, by recombinant means. Further, use of the recombinantly produced neutral protease is disclosed in the field of cell biology, particularly for the purpose of tissue dissociation. The disclosure also includes blends with other proteases. Further disclosed are nucleotide sequences encoding the neutral protease.

公开(公告)号：US20240132866A1

公开(公告)日：2024-04-25

申请号：US18057800

申请日：2022-11-22

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Thomas Greiner-Stoeffele ,  Stefan Schoenert

IPC: C12N9/52 ,  C07K14/195 ,  C12N5/071

CPC classification number: C12N9/52 ,  C07K14/195 ,  C12N5/0602 ,  C12Y304/24 ,  C12Y304/24028 ,  C12N2509/00

Abstract: The present disclosure provides the sequence of a Paenibacillus polymyxa preproenzyme which is the precursor of a neutral protease, expression thereof in a transformed host organism, and methods for production of the neutral protease, by recombinant means. Further, use of the recombinantly produced neutral protease is disclosed in the field of cell biology, particularly for the purpose of tissue dissociation. The disclosure also includes blends with other proteases. Further disclosed are nucleotide sequences encoding the neutral protease.

公开(公告)号：US10526594B2

公开(公告)日：2020-01-07

申请号：US15334658

申请日：2016-10-26

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Thomas Greiner-Stoeffele ,  Stefan Schoenert

IPC: C12N9/54 ,  C12N9/52 ,  C07K14/195 ,  C12N5/071

Abstract: The present disclosure provides the sequence of a Paenibacillus polymyxa preproenzyme which is the precursor of a neutral protease, expression thereof in a transformed host organism, and methods for production of the neutral protease, by recombinant means. Further, use of the recombinantly produced neutral protease is disclosed in the field of cell biology, particularly for the purpose of tissue dissociation. The disclosure also includes blends with other proteases. Further disclosed are nucleotide sequences encoding the neutral protease.

公开(公告)号：US12234491B2

公开(公告)日：2025-02-25

申请号：US18057800

申请日：2022-11-22

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Thomas Greiner-Stoeffele ,  Stefan Schoenert

IPC: C12N9/54 ,  C07K14/195 ,  C12N5/071 ,  C12N9/52 ,  C12N15/57 ,  C12N15/75

Abstract: The present disclosure provides the sequence of a Paenibacillus polymyxa preproenzyme which is the precursor of a neutral protease, expression thereof in a transformed host organism, and methods for production of the neutral protease, by recombinant means. Further, use of the recombinantly produced neutral protease is disclosed in the field of cell biology, particularly for the purpose of tissue dissociation. The disclosure also includes blends with other proteases. Further disclosed are nucleotide sequences encoding the neutral protease.

公开(公告)号：US10889809B2

公开(公告)日：2021-01-12

申请号：US16700268

申请日：2019-12-02

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Thomas Greiner-Stoeffele ,  Stefan Schoenert

IPC: C12N5/02 ,  C12N5/071 ,  C07K14/195 ,  C12N9/52

Abstract: The present disclosure provides the sequence of a Paenibacillus polymyxa preproenzyme which is the precursor of a neutral protease, expression thereof in a transformed host organism, and methods for production of the neutral protease, by recombinant means. Further, use of the recombinantly produced neutral protease is disclosed in the field of cell biology, particularly for the purpose of tissue dissociation. The disclosure also includes blends with other proteases. Further disclosed are nucleotide sequences encoding the neutral protease.

公开(公告)号：US20190203191A1

公开(公告)日：2019-07-04

申请号：US16355259

申请日：2019-03-15

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Thomas Greiner-Stoeffele ,  Stefan Schoenert

IPC: C12N9/52 ,  C07K14/195 ,  C12N5/071

CPC classification number: C12N9/52 ,  C07K14/195 ,  C12N5/0602 ,  C12N2509/00 ,  C12Y304/24 ,  C12Y304/24028

Abstract: The present disclosure provides the sequence of a Paenibacillus polymyxa preproenzyme which is the precursor of a neutral protease, expression thereof in a transformed host organism, and methods for production of the neutral protease, by recombinant means. Further, use of the recombinantly produced neutral protease is disclosed in the field of cell biology, particularly for the purpose of tissue dissociation. The disclosure also includes blends with other proteases. Further disclosed are nucleotide sequences encoding the neutral protease.

公开(公告)号：US20150166973A1

公开(公告)日：2015-06-18

申请号：US14592969

申请日：2015-01-09

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Thomas Greiner-Stoeffele ,  Stefan Schoenert

IPC: C12N9/52 ,  C12N5/071

CPC classification number: C12N9/52 ,  C07K14/195 ,  C12N5/0602 ,  C12N2509/00 ,  C12Y304/24 ,  C12Y304/24028

Abstract: The present disclosure provides the sequence of a Paenibacillus polymyxa preproenzyme which is the precursor of a neutral protease, expression thereof in a transformed host organism, and methods for production of the neutral protease, by recombinant means. Further, use of the recombinantly produced neutral protease is disclosed in the field of cell biology, particularly for the purpose of tissue dissociation. The disclosure also includes blends with other proteases. Further disclosed are nucleotide sequences encoding the neutral protease.

Abstract translation: 本公开内容提供了通过重组方法作为中性蛋白酶的前体，转化的宿主生物体中的表达和中性蛋白酶的生产方法的多粘芽孢杆菌前酶的序列。 此外，在细胞生物学领域中公开了使用重组产生的中性蛋白酶，特别是为了组织解离的目的。 本公开还包括与其它蛋白酶的混合物。 还公开了编码中性蛋白酶的核苷酸序列。

公开(公告)号：US20240228999A9

公开(公告)日：2024-07-11

申请号：US18057800

申请日：2022-11-22

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Thomas Greiner-Stoeffele ,  Stefan Schoenert

IPC: C12N9/52 ,  C07K14/195 ,  C12N5/071

CPC classification number: C12N9/52 ,  C07K14/195 ,  C12N5/0602 ,  C12Y304/24 ,  C12Y304/24028 ,  C12N2509/00

Abstract: The present disclosure provides the sequence of a Paenibacillus polymyxa preproenzyme which is the precursor of a neutral protease, expression thereof in a transformed host organism, and methods for production of the neutral protease, by recombinant means. Further, use of the recombinantly produced neutral protease is disclosed in the field of cell biology, particularly for the purpose of tissue dissociation. The disclosure also includes blends with other proteases. Further disclosed are nucleotide sequences encoding the neutral protease.

公开(公告)号：US11542489B2

公开(公告)日：2023-01-03

申请号：US17386106

申请日：2021-07-27

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Thomas Greiner-Stoeffele ,  Stefan Schoenert

IPC: C12N9/54 ,  C12N15/31 ,  C12N15/57 ,  C12N1/19 ,  C12N9/52 ,  C07K14/195 ,  C12N5/071

Abstract: The present disclosure provides the sequence of a Paenibacillus polymyxa preproenzyme which is the precursor of a neutral protease, expression thereof in a transformed host organism, and methods for production of the neutral protease, by recombinant means. Further, use of the recombinantly produced neutral protease is disclosed in the field of cell biology, particularly for the purpose of tissue dissociation. The disclosure also includes blends with other proteases. Further disclosed are nucleotide sequences encoding the neutral protease.

公开(公告)号：US20170037390A1

公开(公告)日：2017-02-09

申请号：US15334658

申请日：2016-10-26

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Thomas Greiner-Stoeffele ,  Stefan Schoenert

IPC: C12N9/52

CPC classification number: C12N9/52 ,  C07K14/195 ,  C12N5/0602 ,  C12N2509/00 ,  C12Y304/24 ,  C12Y304/24028

Abstract: The present disclosure provides the sequence of a Paenibacillus polymyxa preproenzyme which is the precursor of a neutral protease, expression thereof in a transformed host organism, and methods for production of the neutral protease, by recombinant means. Further, use of the recombinantly produced neutral protease is disclosed in the field of cell biology, particularly for the purpose of tissue dissociation. The disclosure also includes blends with other proteases. Further disclosed are nucleotide sequences encoding the neutral protease.

Abstract translation: 本公开内容提供了通过重组方法作为中性蛋白酶的前体，转化的宿主生物体中的表达和中性蛋白酶的生产方法的多粘芽孢杆菌前酶的序列。 此外，在细胞生物学领域中公开了使用重组产生的中性蛋白酶，特别是为了组织解离的目的。 本公开还包括与其它蛋白酶的混合物。 还公开了编码中性蛋白酶的核苷酸序列。