公开(公告)号：US10982274B2

公开(公告)日：2021-04-20

申请号：US16249681

申请日：2019-01-16

申请人： Roche Molecular Systems, Inc.

发明人： Jan Berka ,  Yi-Ju Chen ,  John H. Leamon ,  Steven Lefkowitz ,  Kenton L. Lohman ,  Vinod B. Makhijani ,  Jonathan M. Rothberg ,  Gary J. Sarkis ,  Maithreyan Srinivasan ,  Michael P. Weiner

IPC分类号： C12Q1/68 ,  B01L3/00 ,  C07H21/00 ,  C12N15/10 ,  G01N21/25 ,  G01N21/64 ,  G01R33/12 ,  C12Q1/686 ,  C12Q1/6834 ,  C12Q1/6844 ,  C12Q1/6865 ,  C12Q1/6867 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6806 ,  B01L7/00

摘要： Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.