公开(公告)号：US10752658B2

公开(公告)日：2020-08-25

申请号：US15762092

申请日：2016-09-20

Applicant: ROCHE SEQUENCING SOLUTIONS, INC.

Inventor： Cynthia Cech ,  Tim Craig ,  Christos Tzitzilonis ,  Alexander Yang ,  Liv Jensen ,  Charlotte Yang ,  Corissa Harris ,  Matthew DiPietro ,  Dhruti Dalal

IPC: C07K14/245 ,  C12Q1/6874 ,  C12Q1/68 ,  G01N33/68 ,  C12N9/12

Abstract: The present disclosure provides variant OmpG polypeptides, compositions comprising the OmpG variant polypeptides, and methods for using the variant OmpG polypeptides as nanopores for determining the sequence of single stranded nucleic acids. The variant OmpG nanopores reduce the ionic current noise versus the parental OmpG polypeptide from which they are derived and thereby enable sequencing of polynucleotides with single nucleotide resolution. The reduced ionic current noise also provides for the use of these OmpG nanopore variants in other single molecule sensing applications, e.g., protein sequencing.

公开(公告)号：US11866464B2

公开(公告)日：2024-01-09

申请号：US17226378

申请日：2021-04-09

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Dmitriy Gremyachinskiy ,  Christos Tzitzilonis

IPC: C07K14/315 ,  C07K1/107 ,  A61K47/64 ,  C07K19/00 ,  C12N9/12 ,  C12N9/52 ,  C12N15/62 ,  C12Q1/68

CPC classification number: C07K14/315 ,  A61K47/64 ,  A61K47/6415 ,  C07K1/1072 ,  C07K1/1077 ,  C07K19/00 ,  C12N9/1205 ,  C12N9/1241 ,  C12N9/52 ,  C12N15/62 ,  C12Q1/68 ,  C07K2319/03 ,  C07K2319/20 ,  C12Y207/07

Abstract: The present disclosure relates to relates methods and associated compositions that provide fast, efficient site-selective conjugation of a protein, such as the pore-forming protein α-hemolysin, to a biomolecule, such as a DNA polymerase, and the use of such site-selective protein-biomolecule conjugates in nanopore devices and methods.

公开(公告)号：US11261488B2

公开(公告)日：2022-03-01

申请号：US16917435

申请日：2020-06-30

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Timothy Kellogg Craig ,  Cynthia Ann Cech ,  Michael Dorwart ,  Liv Elisabeth Jensen ,  Marshall Winston Porter ,  Christos Tzitzilonis ,  Alexander Hyun-min Yang

IPC: C07K14/31 ,  C12Q1/6869

Abstract: Described herein are engineered alpha-hemolysin subunits having mutated oligomerization domains for assembling into heptameric nanopores in lipid bilayers.

公开(公告)号：US12037366B2

公开(公告)日：2024-07-16

申请号：US18449904

申请日：2023-08-15

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Cynthia Cech ,  Tim Craig ,  Christos Tzitzilonis ,  Alexander Yang ,  Liv Jensen ,  Charlotte Yang ,  Corissa Harris ,  Matthew Dipietro ,  Dhruti Dalal

IPC: C12Q1/68 ,  A61K39/02 ,  C07K14/245 ,  C12N9/12 ,  C12Q1/6874 ,  G01N33/68

CPC classification number: C07K14/245 ,  C12N9/1252 ,  C12Q1/68 ,  C12Q1/6874 ,  C12Y207/07007 ,  G01N33/6803 ,  C07K2319/00 ,  C12Q1/6874 ,  C12Q2525/197 ,  C12Q2525/203 ,  C12Q2565/631

Abstract: The present disclosure provides variant OmpG polypeptides, compositions comprising the OmpG variant polypeptides, and methods for using the variant OmpG polypeptides as nanopores for determining the sequence of single stranded nucleic acids. The variant OmpG nanopores reduce the ionic current noise versus the parental OmpG polypeptide from which they are derived and thereby enable sequencing of polynucleotides with single nucleotide resolution. The reduced ionic current noise also provides for the use of these OmpG nanopore variants in other single molecule sensing applications, e.g., protein sequencing.

公开(公告)号：US11001611B2

公开(公告)日：2021-05-11

申请号：US16134179

申请日：2018-09-18

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Dmitriy Gremyachinskiy ,  Christos Tzitzilonis

IPC: C07K14/315 ,  C07K1/107 ,  C12Q1/68 ,  A61K47/64 ,  C07K19/00 ,  C12N9/12 ,  C12N9/52 ,  C12N15/62

Abstract: The present disclosure relates to relates methods and associated compositions that provide fast, efficient site-selective conjugation of a protein, such as the pore-forming protein α-hemolysin, to a biomolecule, such as a DNA polymerase, and the use of such site-selective protein-biomolecule conjugates in nanopore devices and methods.

公开(公告)号：US11767348B2

公开(公告)日：2023-09-26

申请号：US16925848

申请日：2020-07-10

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Cynthia Cech ,  Tim Craig ,  Christos Tzitzilonis ,  Alexander Yang ,  Liv Jensen ,  Charlotte Yang ,  Corissa Harris ,  Matthew Dipietro ,  Dhruti Dalal

IPC: A61K39/02 ,  C07K14/245 ,  C12Q1/6874 ,  C12Q1/68 ,  G01N33/68 ,  C12N9/12

CPC classification number: C07K14/245 ,  C12N9/1252 ,  C12Q1/68 ,  C12Q1/6874 ,  C12Y207/07007 ,  G01N33/6803 ,  C07K2319/00 ,  C12Q1/6874 ,  C12Q2525/197 ,  C12Q2525/203 ,  C12Q2565/631

Abstract: The present disclosure provides variant OmpG polypeptides, compositions comprising the OmpG variant polypeptides, and methods for using the variant OmpG polypeptides as nanopores for determining the sequence of single stranded nucleic acids. The variant OmpG nanopores reduce the ionic current noise versus the parental OmpG polypeptide from which they are derived and thereby enable sequencing of polynucleotides with single nucleotide resolution. The reduced ionic current noise also provides for the use of these OmpG nanopore variants in other single molecule sensing applications, e.g., protein sequencing.

公开(公告)号：US10752948B2

公开(公告)日：2020-08-25

申请号：US16238874

申请日：2019-01-03

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Timothy Kellogg Craig ,  Cynthia Ann Cech ,  Michael Dorwart ,  Liv Elisabeth Jensen ,  Marshall Winston Porter ,  Christos Tzitzilonis ,  Alexander Hyun-min Yang

IPC: C07K14/31 ,  C12Q1/6869

Abstract: Described herein are engineered alpha-hemolysin subunits having mutated oligomerization domains for assembling into heptameric nanopores in lipid bilayers.