公开(公告)号：US07060460B2

公开(公告)日：2006-06-13

申请号：US10261508

申请日：2002-10-02

申请人： Roman Necina ,  Robert Schlegl ,  Alois Jungbauer ,  Christine Machold

发明人： Roman Necina ,  Robert Schlegl ,  Alois Jungbauer ,  Christine Machold

IPC分类号： C12P21/06

CPC分类号： C07K1/1136 ,  C07K1/16

摘要： In a method for reconstituting a recombinant protein from a denatured state to its active form, a feed solution containing the recombinant protein in its denatured and/or in biologically inactive intermediate forms is subjected to a chromatographic separation process, in which the protein is reconstituted under conditions that promote refolding of the protein and the intermediate forms are separated from the refolded protein. The denatured form and/or the inactive intermediate forms of the protein are separated from the refolded protein in a continuous or quasi-continuous manner and optionally recycled to the feed solution.

公开(公告)号：US20050233365A1

公开(公告)日：2005-10-20

申请号：US11101747

申请日：2005-04-08

申请人： Hans Huber ,  Claudia Pacher ,  Roman Necina ,  Franz Kollmann ,  Christoph Reinisch

发明人： Hans Huber ,  Claudia Pacher ,  Roman Necina ,  Franz Kollmann ,  Christoph Reinisch

IPC分类号： C12N15/70 ,  C12P19/04 ,  C12P19/34 ,  C12Q1/68

CPC分类号： C12P19/34 ,  C12N15/70

摘要： A method for producing plasmid DNA on a manufacturing scale uses Escherichia coli K-12 strain JM108. The process results in high yield and homogeneity of plasmid DNA.

摘要翻译： 制造规模生产质粒DNA的方法使用大肠杆菌K-12菌株JM108。 该过程导致质粒DNA的高产量和均一性。

公开(公告)号：US20050148507A1

公开(公告)日：2005-07-07

申请号：US10833656

申请日：2004-04-28

申请人： Robert Wandl ,  Roman Necina ,  Henri Doods ,  Martin Lenter ,  Randolph Seidler

发明人： Robert Wandl ,  Roman Necina ,  Henri Doods ,  Martin Lenter ,  Randolph Seidler

IPC分类号： A61K38/17 ,  C07K14/475 ,  C07K14/52

CPC分类号： C07K14/523

摘要： The invention relates to a process for preparing pyroGlu-MCP-1 from recombinantly produced Gln-MCP-1, wherein Gln-MCP-1 is incubated at a temperature in the range from 30° C. and 80° C. in a buffer solution with a salt concentration in the range from 10 mM to 160 mM and a pH in the range from 2 to 7.5, until at least 90% of the MCP-1 is present in the form of the pyroGlu-MCP-1.

摘要翻译： 本发明涉及从重组产生的Gln-MCP-1制备pyroGlu-MCP-1的方法，其中Gln-MCP-1在30℃和80℃的温度下在缓冲溶液 盐浓度范围为10mM至160mM，pH范围为2至7.5，直至MCP-1的至少90％以pyroGlu-MCP-1的形式存在。

公开(公告)号：US08501402B2

公开(公告)日：2013-08-06

申请号：US10806346

申请日：2004-03-23

申请人： Jochen Urthaler ,  Roman Necina ,  Christine Ascher ,  Helga Woehrer

发明人： Jochen Urthaler ,  Roman Necina ,  Christine Ascher ,  Helga Woehrer

IPC分类号： C12Q1/68 ,  G01N33/00

CPC分类号： C12N15/1006

摘要： A scalable process and device for producing a biomolecule, in particular pharmaceutical grade plasmid DNA. The process includes the steps of alkaline lysis and a neutralization. For separating the lysate and the precipitate, the mixture is allowed to gently flow downward through a clarification reactor that is partially filled, in its lower part, with retention material like glass beads, whereby the precipitate is retained on top of and within the retention. In a preferred embodiment of the lysis step, cell suspension and alkaline lysis solution flow through a lysis reactor that is filled with particulate material like glass beads. The process can be run continuously and fully automated.

摘要翻译： 用于生产生物分子，特别是药物级质粒DNA的可扩展的方法和装置。 该方法包括碱裂解和中和的步骤。 为了分离裂解物和沉淀物，将混合物轻轻地向下流过澄清反应器，澄清反应器在其下部部分地填充有诸如玻璃珠的保留材料，由此沉淀物保留在保留物的顶部和内部。 在裂解步骤的优选实施方案中，细胞悬浮液和碱性裂解溶液流过填充有诸如玻璃珠的颗粒材料的裂解反应器。 该过程可以连续运行并完全自动化。

公开(公告)号：US20050026177A1

公开(公告)日：2005-02-03

申请号：US10806346

申请日：2004-03-23

申请人： Jochen Urthaler ,  Roman Necina ,  Christine Ascher ,  Helga Woehrer

发明人： Jochen Urthaler ,  Roman Necina ,  Christine Ascher ,  Helga Woehrer

IPC分类号： C12N1/08 ,  C12N15/10 ,  C12Q1/68

CPC分类号： C12N15/1006

摘要： A scalable process and device for producing a biomolecule, in particular pharmaceutical grade plasmid DNA. The process includes the steps of alkaline lysis and a neutralization. For separating the lysate and the precipitate, the mixture is allowed to gently flow downward through a clarification reactor that is partially filled, in its lower part, with retention material like glass beads, whereby the precipitate is retained on top of and within the retention. In a preferred embodiment of the lysis step, cell suspension and alkaline lysis solution flow through a lysis reactor that is filled with particulate material like glass beads. The process can be run continuosly and fully automated.

摘要翻译： 用于生产生物分子，特别是药物级质粒DNA的可扩展的方法和装置。 该方法包括碱裂解和中和的步骤。 为了分离裂解物和沉淀物，将混合物轻轻地向下流过澄清反应器，澄清反应器在其下部部分地填充有诸如玻璃珠的保留材料，由此沉淀物保留在保留物的顶部和内部。 在裂解步骤的优选实施方案中，细胞悬浮液和碱性裂解溶液流过填充有诸如玻璃珠的颗粒材料的裂解反应器。 该过程可以连续运行并完全自动化。