公开(公告)号：US5866378A

公开(公告)日：1999-02-02

申请号：US731189

申请日：1996-10-07

申请人： Ruediger Marquardt ,  Brigitte Hoersch ,  Andreas Seiffert-Stoeriko ,  Andreas Stein ,  Astrid Zervosen ,  Lothar Elling ,  Maria Regina Kula ,  Stefan Verseck ,  Juergen Distler ,  Wolfgang Piepersberg

发明人： Ruediger Marquardt ,  Brigitte Hoersch ,  Andreas Seiffert-Stoeriko ,  Andreas Stein ,  Astrid Zervosen ,  Lothar Elling ,  Maria Regina Kula ,  Stefan Verseck ,  Juergen Distler ,  Wolfgang Piepersberg

IPC分类号： C07H19/04 ,  C07H19/10 ,  C07H19/20 ,  C12P19/30 ,  C12P19/02 ,  C12N11/18 ,  C12P19/04

CPC分类号： C12P19/30 ,  C07H19/04

摘要： The present invention relates to processes for the enzymatic synthesis of nucleotide-6-deoxy-D-xylo-4-hexuloses starting from a nucleoside monophosphate (NMP). These processes comprise simultaneous incubation of the following substances in a buffer solution:(a) substrates comprising a nucleoside monophosphate, phosphoenolpyruvate, adenosine triphosphate, and sucrose; and(b) enzymes comprising pyruvate kinase, nucleoside-monophosphate kinase, sucrose synthase and deoxythymidine-D-glucose 4,6dehydratase.

摘要翻译： 本发明涉及从核苷单磷酸（NMP）开始的酶合成核苷酸-6-脱氧-D-木糖-4-己糖苷的方法。 这些方法包括将以下物质同时孵育在缓冲溶液中：（a）包含核苷单磷酸，磷酸烯醇丙酮酸，三磷酸腺苷和蔗糖的底物; 和（b）包含丙酮酸激酶，核苷单磷酸激酶，蔗糖合成酶和脱氧胸苷-D-葡萄糖四氢叶酸酶的酶。

公开(公告)号：US5811539A

公开(公告)日：1998-09-22

申请号：US520690

申请日：1995-08-30

申请人： Andreas Seiffert-Stoeriko ,  Brigitte Hoersch ,  Ruediger Marquardt ,  Gerhard Kretzschmar ,  Johannes Meiwes

发明人： Andreas Seiffert-Stoeriko ,  Brigitte Hoersch ,  Ruediger Marquardt ,  Gerhard Kretzschmar ,  Johannes Meiwes

IPC分类号： C07H1/08 ,  C07H19/06 ,  C12P19/30 ,  C07H19/16

CPC分类号： C12P19/305 ,  C12P19/30

摘要： The present invention relates to an improved process for isolating and purifying sugar nucleotides, in particular cytidine monophosphate-activated N-acetylneuraminic acid (CMP-Nana), from biological sources. The sugar nucleotides can be obtained from a sugar nucleotide-containing solution, which has been freed of proteins by alcoholic precipitation, by means of an improved column-chromatographic method which uses silica gel as the stationary phase, and at a purity level which requires only a subsequent desalting step to obtain a substantially pure product.

摘要翻译： 本发明涉及从生物来源分离和纯化糖核苷酸，特别是胞苷苷一磷酸酯活化的N-乙酰神经氨酸（CMP-Nana）的改进方法。 糖核苷酸可以通过使用硅胶作为固定相的改进的柱色谱方法从已经通过酒精沉淀而不含蛋白质的含糖核苷酸的溶液获得，并且纯度仅需要 随后的脱盐步骤以获得基本上纯的产物。

公开(公告)号：US06406894B1

公开(公告)日：2002-06-18

申请号：US08989136

申请日：1997-12-11

申请人： Brigitte Hoersch ,  Michael Ahlers ,  Gerhard Kretzschmar ,  Eckart Bartnik ,  Dick Seiffge

发明人： Brigitte Hoersch ,  Michael Ahlers ,  Gerhard Kretzschmar ,  Eckart Bartnik ,  Dick Seiffge

IPC分类号： C12P1918

CPC分类号： C07H15/203 ,  A61K47/595 ,  A61K47/61 ,  A61K47/64 ,  C07H3/06 ,  C07H15/04 ,  C12P19/18 ,  C12P19/26 ,  C12P19/44

摘要： A process for preparing polyvalent, physiologically degradable carbohydrate-containing polymers by enzymatic glycosylation reactions is described. The carbohydrate-containing polymers thus prepared may be used for preparing carbohydrate building blocks. The polyvalent carbohydrate-containing polymers of the invention cause no intolerance reactions in vivo, either in their intact form or in the form of degradation products. The carbohydrate side chain of the carbohydrate-containing polymer is assembled by enzymatic glycosylation reactions in homogeneous aqueous buffer systems directly on a biodegradable polymer. The yields of the glycosylation reaction are significantly improved over known processes, and are often quantitative. This also provides a significant increase in the loading densities. A process for preparing free oligosaccharides by means of the carbohydrate-containing polymers of the invention is also described.

摘要翻译： 描述了通过酶促糖基化反应制备含多价，生理可降解的含碳水化合物的聚合物的方法。 如此制备的含碳水化合物的聚合物可用于制备碳水化合物结构单元。 本发明的含多价碳水化合物的聚合物在体内不会以其完整形式或降解产物的形式引起不耐受反应。 含碳水化合物的聚合物的碳水化合物侧链通过酶促糖基化反应在直接在可生物降解的聚合物上的均相含水缓冲体系中组装。 糖基化反应的产率比已知方法显着改善，并且通常是定量的。 这也显着增加了装载密度。 还描述了通过本发明的含碳水化合物的聚合物制备游离寡糖的方法。