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公开(公告)号：US5702916A
公开(公告)日：1997-12-30
申请号：US449958
申请日：1995-05-25
摘要： A replicon, in which a nucleotide sequence encoding a cell killing function is regulatably expressed when the replicon is harbored in one type of host cell (primary host cell), so that cells harboring the replicon are killed under conditions under which the cell killing function is expressed, and the nucleotide sequence encoding the cell killing function is regulatably or constitutively expressed when the replicon is harbored in another type of host cell (secondary host cell), so that cells harboring the replicon are invariably killed or killed under conditions under which the cell killing function is expressed, may be used in a method of active biological containment of cells under defined environmental conditions. The biological containment principle may be utilized in the industrial production of a biosynthetic product by recombinant DNA techniques, when deliberately releasing a genetically engineered microorganism to the natural environment or in the preparation of a live vaccine. The expression of the cell killing function may be regulated by means of a promoter.
摘要翻译： 当复制子存在于一种类型的宿主细胞（主要宿主细胞）中时，可复制表达编码细胞杀伤功能的核苷酸序列的复制子，使得携带复制子的细胞在细胞杀伤功能为 表达，并且当复制子宿主在另一种类型的宿主细胞（次级宿主细胞）中时，编码细胞杀伤功能的核苷酸序列是可调节的或组成型表达的，使得携带复制子的细胞在细胞的条件下总是被杀死或杀死 表达杀伤功能，可用于在确定的环境条件下对细胞的活性生物遏制的方法。 当将遗传工程微生物故意释放到天然环境或活疫苗的制备中时，可通过重组DNA技术在生物合成产物的工业生产中利用生物遏制原理。 细胞杀伤功能的表达可以通过启动子调节。
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公开(公告)号：US5853718A
公开(公告)日：1998-12-29
申请号：US434353
申请日：1995-05-02
IPC分类号： C12N15/09 , A61K39/00 , C07K14/245 , C12N1/00 , C12N15/00 , A61K39/02 , A61K39/002 , A61K39/108 , A61K39/12
CPC分类号： C07K14/245 , C12N15/00 , A61K39/00
摘要： A vertebrate animal, such as a human, is immunized with a bacterial host cell harboring a recombinant replicon which provides a stochastically expressed cell killing function whereby the cells are biologically contained. The replicon also comprises a gene encoding an antigen which is to be displayed on the outer surface of the host cell, so that it can elicit an immune response from the immunized animal. This antigen comprises one or more epitopes from a pathogenic agent.
摘要翻译： 脊椎动物，例如人，用携带重组复制子的细菌宿主细胞免疫，其提供随机表达的细胞杀伤功能，由此细胞被生物地包含。 复制子还包含编码要在宿主细胞的外表面上显示的抗原的基因，从而可以引起免疫动物的免疫应答。 该抗原包含来自病原体的一个或多个表位。
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公开(公告)号：US5670370A
公开(公告)日：1997-09-23
申请号：US452494
申请日：1995-05-30
CPC分类号： C07K14/245 , C12N15/00 , A61K39/00
摘要： A replicon, in which a nucleotide sequence encoding a cell killing function is regulatably expressed when the replicon is harboured in one type of host cell (primary host cell), so that cells harbouring the replicon are killed under conditions under which the cell killing function is expressed, and the nucleotide sequence encoding the cell killing function is regulatably or constitutively expressed when the replicon is harboured in another type of host cell (secondary host cell), so that cells harbouring the replicon are invariably killed or killed under conditions under which the cell killing function is expressed, may be used in a method of active biological containment of cells under defined environmental conditions. The biological containment principle may be utilized in the industrial production of a biosynthetic product by recombinant DNA techniques, when deliberately releasing a genetically engineered microorganism to the natural environment or in the preparation of a live vaccine. The expression of the cell killing function may be regulated by means of a promoter.
摘要翻译： 当复制子存在于一种类型的宿主细胞（主要宿主细胞）中时，可复制表达编码细胞杀伤功能的核苷酸序列的复制子，使得携带复制子的细胞在细胞杀伤功能为 表达，并且当复制子宿主在另一种类型的宿主细胞（次级宿主细胞）中时，编码细胞杀伤功能的核苷酸序列是可调节的或组成型表达的，使得携带复制子的细胞在细胞的条件下总是被杀死或杀死 表达杀伤功能，可用于在确定的环境条件下对细胞的活性生物遏制的方法。 当将遗传工程微生物故意释放到天然环境或活疫苗的制备中时，可通过重组DNA技术在生物合成产物的工业生产中利用生物遏制原理。 细胞杀伤功能的表达可以通过启动子调节。
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公开(公告)号：US5545541A
公开(公告)日：1996-08-13
申请号：US406880
申请日：1989-09-13
CPC分类号： C12N15/68 , C12N15/67 , Y10S435/874 , Y10S435/88
摘要： The stable maintenance of a replicon in a population of growing cells is ensured by providing the replicon with a sequence which encodes a product capable of killing the cell harboring the replicon or the progeny of the cell (or encodes a precursor for the product) and a sequence encoding an antagonist for the killing product (or a precursor for the antagonist). The antagonist is one which suppresses the killing product (or a precursor for the killing product) in cells harboring the replicon, whereas the antagonist activity decays when the replicon is lost from the cell so that the antagonist (or its precursor) is no longer continuously expressed. This means that the killing product (or its precursor) present in the now replicon-free cell is no longer suppressed by the antagonist, resulting in cell death.Cells containing the thus stabilized replicon may be grown on a large scale without any significant loss of the replicon from the cell population even when no selection pressure is applied.
摘要翻译： 通过向复制子提供编码能够杀死携带复制子或细胞后代（或编码产物前体）的细胞的产物的序列，确保复制子在生长细胞群中的稳定维持 编码杀伤产品（或拮抗剂前体）的拮抗剂的序列。 拮抗剂是抑制在复制子的细胞中杀死产物（或杀死产物的前体）的拮抗剂，而当复制子从细胞中丢失时，拮抗剂活性衰减，使得拮抗剂（或其前体）不再连续 表达。 这意味着现在无复制子细胞中存在的杀死产品（或其前体）不再被拮抗剂抑制，导致细胞死亡。 即使没有施加选择压力，含有如此稳定的复制子的细胞可以大规模地生长，而不会从细胞群体中复制子的任何显着的损失。
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