-
公开(公告)号：US20180259527A1
公开(公告)日：2018-09-13
申请号：US15984288
申请日：2018-05-18
发明人： Hikaru Mizumura , Maki Aizawa , Toshio Oda
IPC分类号： G01N33/579
CPC分类号： G01N33/579 , C12Q1/34 , C12Q1/37 , G01N2333/195 , G01N2333/43508 , G01N2333/96411 , G01N2400/50
摘要： A method for rapidly and highly sensitively measuring endotoxin relies on an endotoxin-measuring agent, which includes a factor C derived from Tachypleus tridentatus that does not have His-tag sequence at the C-terminus, a factor B of a horseshoe crab, and a proclotting enzyme of a horseshoe crab. Each of these proteins can be a recombinant protein obtainable by being expressed using a stably expressing cell line of an insect cell as a host:
-
2.发明授权公开(公告)号：US11236318B2
公开(公告)日：2022-02-01
申请号：US16399386
申请日：2019-04-30
IPC分类号： C12N9/64 , G01N33/569 , C12Q1/37 , C12N15/85 , G01N33/579
摘要： A horseshoe crab Factor C protein having activity of Factor C, wherein the horseshoe crab is selected from Tachypleus tridentatus, Limulus polyphemus, and Carcinoscorpius rotundicauda, and wherein the horseshoe crab Factor C protein is produced through being recombinantly expressed from a Chinese Hamster Ovary (CHO) DG44 cell or HEK cell.
-
3.发明授权公开(公告)号：US10144923B2
公开(公告)日：2018-12-04
申请号：US14650767
申请日：2013-12-10
IPC分类号： C12N9/64 , G01N33/569 , G01N33/579 , C12Q1/37 , C12Q1/04
摘要： To provide a method for producing a horseshoe crab recombinant Factor C. The horseshoe crab recombinant Factor C is produced through expression thereof by use of mammalian cells such as CHO DG44 and HEK293 as host cells.
-
4.发明申请公开(公告)号：US20180258414A1
公开(公告)日：2018-09-13
申请号：US15983725
申请日：2018-05-18
IPC分类号： C12N9/64 , G01N33/569 , G01N33/579
CPC分类号： C12N9/6408 , C12Y304/21069 , C12Y304/21084 , G01N33/56911 , G01N33/579 , G01N2333/96411
摘要： To provide a method for producing a horseshoe crab recombinant Factor C. The horseshoe crab recombinant Factor C is produced through expression thereof by use of mammalian cells such as CHO DG44 and HEK293 as host cells.
-
5.发明授权公开(公告)号：US11959109B2
公开(公告)日：2024-04-16
申请号：US18346420
申请日：2023-07-03
IPC分类号： C12N9/64 , C12Q1/37 , G01N33/569 , G01N33/579
CPC分类号： C12N9/6408 , C12Y304/21069 , C12Y304/21084 , G01N33/56911 , G01N33/579 , G01N2333/96411
摘要： A horseshoe crab Factor C protein having activity of Factor C, wherein the horseshoe crab is selected from Tachypleus tridentatus, Limulus polyphemus, and Carcinoscorpius rotundicauda, and wherein the horseshoe crab Factor C protein is produced through being recombinantly expressed from a Chinese Hamster Ovary (CHO) DG44 cell or HEK cell.
-
公开(公告)号：US10883963B2
公开(公告)日：2021-01-05
申请号：US16076614
申请日：2017-02-15
发明人： Hikaru Mizumura , Toshio Oda , Tomokazu Matsue , Kumi Inoue
IPC分类号： G01N27/48 , C07C211/51 , G01N33/579 , C07K9/00 , G01N33/487 , C07K14/195 , G01N27/447
摘要： The present invention addresses the problem of providing a means for making highly sensitive and stable measurements possible using electrochemical measurement methods. This problem is resolved by providing: a labeling substance represented by general formula (1) and used to label a substrate; a measurement substrate that is formed by being labeled using the labeling substance; a method of measuring using electrochemical measurement methods that use the measurement substrate; and a reagent kit that includes as components the labeling substance and/or the measurement substrate. (In general formula (1), R1 is either H or an alkyl group (CmH2m+1), m is an integer of 1-4, R2 is an alkyl group (CnH2n+1), and n is an integer of 1-4.)
-
公开(公告)号：US11162949B2
公开(公告)日：2021-11-02
申请号：US15984288
申请日：2018-05-18
发明人： Hikaru Mizumura , Maki Aizawa , Toshio Oda
IPC分类号： C12Q1/34 , C12Q1/37 , G01N33/579
摘要： A method for rapidly and highly sensitively measuring endotoxin relies on an endotoxin-measuring agent, which includes a factor C derived from Tachypleus tridentatus that does not have His-tag sequence at the C-terminus, a factor B of a horseshoe crab, and a proclotting enzyme of a horseshoe crab. Each of these proteins can be a recombinant protein obtainable by being expressed using a stably expressing cell line of an insect cell as a host.
-
8.发明授权公开(公告)号：US10982202B2
公开(公告)日：2021-04-20
申请号：US15983725
申请日：2018-05-18
IPC分类号： C12N9/64 , C12N5/07 , C12N5/10 , A61K38/48 , G01N33/579 , G01N33/569
摘要： To provide a method for producing a horseshoe crab recombinant Factor C. The horseshoe crab recombinant Factor C is produced through expression thereof by use of mammalian cells such as CHO DG44 and HEK293 as host cells.
-
公开(公告)号：US20170138945A1
公开(公告)日：2017-05-18
申请号：US15359058
申请日：2016-11-22
发明人： Hikaru Mizumura , Maki Aizawa , Toshio Oda
IPC分类号： G01N33/579
CPC分类号： G01N33/579 , C12Q1/34 , C12Q1/37 , G01N2333/195 , G01N2333/43508 , G01N2333/96411 , G01N2400/50
摘要： A method for rapidly and highly sensitively measuring endotoxin is provided. Endotoxin is measured using an endotoxin-measuring agent comprising the proteins (1) to (3) below, each of which is a recombinant protein obtainable by being expressed using insect cells as a host: (1) a factor C derived from Tachypleus tridentatus, which factor C does not have a His-tag sequence at the C-terminus; (2) a factor B of a horseshoe crab; and (3) a proclotting enzyme of a horseshoe crab.
-
公开(公告)号：US11499177B2
公开(公告)日：2022-11-15
申请号：US16490100
申请日：2018-03-01
摘要： Provided is a technology related to a horseshoe crab factor B variant, and also provided is means for performing endotoxin measurement with high sensitivity. A polypeptide having an amino acid sequence in which the amino acid residue at the 193-position in an amino acid sequence of a polypeptide of horseshoe crab factor B is substituted with a cysteine (Cys) residue, is produced. Endotoxin measurement can be carried out with high sensitivity by configuring a Limulus reagent by combining this polypeptide with horseshoe crab factor C.
-
-
-
-
-
-
-
-
-
搜索结果
12 条记录 (耗时 0.016 秒)
