公开(公告)号：US10215759B2

公开(公告)日：2019-02-26

申请号：US15257517

申请日：2016-09-06

Applicant: SENOMYX, INC.

Inventor： Guy Servant ,  Hong Chang ,  Cyril Redcrow ,  Sumita Ray ,  Imran Clark ,  Bryan Moyer

IPC: C12Q1/02 ,  C12N15/85 ,  G01N33/50 ,  G01N33/68

Abstract: In one aspect, the present invention relates to a mammalian cell-based high-throughput assay for the profiling and screening of human epithelial sodium channel (hENaC) cloned from a human kidney c-DNA library and is also expressed in other tissues including human taste tissue. The present invention further relates to amphibian oocyte-based medium-throughput electrophysiological assays for identifying human ENaC modulators, preferably ENaC enhancers. Compounds that modulate ENaC function in a cell-based ENaC assay are expected to affect salty taste in humans. The assays described herein have advantages over existing cellular expression systems. In the case of mammalian cells, such assays can be run in standard 96 or 384 well culture plates in high-throughput mode with enhanced assay results being achieved by the use of a compound that inhibits ENaC function, preferably an amiloride derivative such as Phenamil. In the case of the inventive oocyte electrophysiological assays (two-electrode voltage-clamp technique), these assays facilitate the identification of compounds which specifically modulate human ENaC. The assays of the invention provide a robust screen useful to detect compounds that facilitate (enhance) or inhibit hENaC function. Compounds that enhance or block human ENaC channel activity should thereby modulate salty taste in humans.

公开(公告)号：US20170356909A1

公开(公告)日：2017-12-14

申请号：US15681755

申请日：2017-08-21

Applicant: SENOMYX, INC.

Inventor： Bryan Moyer ,  Albert Zlotnik ,  Peter Hevezi ,  Hortensia Soto ,  Dalia Kalabat ,  Min Lu ,  Na Gao ,  Evan Carl White

IPC: G01N33/566 ,  G01N33/68 ,  C40B30/04 ,  G01N33/50 ,  C40B40/10 ,  C12Q1/68

CPC classification number: G01N33/566 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12Q1/6883 ,  C12Q2600/136 ,  C12Q2600/158 ,  C40B30/04 ,  C40B40/10 ,  G01N33/5005 ,  G01N33/5044 ,  G01N33/6872 ,  G01N2333/726 ,  G01N2500/10

Abstract: This invention relates to novel rationale and methods for identifying human and primate taste-specific genes, including genes involved in salty taste perception, especially human salty taste perception, but also genes involved in sweet, bitter, umami, and sour taste perception, and genes involved in other taste cell or taste receptor related activities such as digestive function and digestive related diseases, taste cell turnover, immunoregulation of the oral and digestive tract, and metabolic regulation such as in diabetes and obesity, the genes identified using these methods, and assays for identifying taste modulators (enhancers or blockers) and potential therapeutics using these genes. These compounds have potential application in modulating (enhancing or blocking) taste perception, especially salty taste perception and as potential therapeutics. In addition, this invention relates to novel methods for identifying taste-specific genes that can be used as markers for different taste cell types, including sweet, bitter, umami, sour, salty, and other taste cells in mammals as well as assays that measure the activity of the sweet, bitter, umami, or sour receptor in the presence of these genes to identify modulators of sweet, bitter, umami, and sour taste and to identify therapeutics especially for treating digestive or metabolic disorders, taste loss, and oral infections. Particularly, the genes identified herein and antibodies or oligos thereto can be used as markers to identify and/or purify specific taste cells e.g., from taste cell suspensions by use of FACS or magnetic bead cell selection or other known cell purification and isolation procedures.

公开(公告)号：US09739776B2

公开(公告)日：2017-08-22

申请号：US14824936

申请日：2015-08-12

Applicant: SENOMYX, INC.

Inventor： Bryan Moyer ,  Albert Zlotnik ,  Peter Hevezi ,  Hortensia Soto ,  Dalia Kalabat ,  Min Lu ,  Na Gao ,  Evan Carl White

IPC: C12N5/071 ,  G01N33/566 ,  C12Q1/68 ,  C40B30/04 ,  C40B40/10 ,  G01N33/50 ,  G01N33/68

CPC classification number: G01N33/566 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12Q1/6883 ,  C12Q2600/136 ,  C12Q2600/158 ,  C40B30/04 ,  C40B40/10 ,  G01N33/5005 ,  G01N33/5044 ,  G01N33/6872 ,  G01N2333/726 ,  G01N2500/10

Abstract: This invention relates to novel rationale and methods for identifying human and primate taste-specific genes, including genes involved in salty taste perception, especially human salty taste perception, but also genes involved in sweet, bitter, umami, and sour taste perception, and genes involved in other taste cell or taste receptor related activities such as digestive function and digestive related diseases, taste cell turnover, immunoregulation of the oral and digestive tract, and metabolic regulation such as in diabetes and obesity, the genes identified using these methods, and assays for identifying taste modulators (enhancers or blockers) and potential therapeutics using these genes. These compounds have potential application in modulating (enhancing or blocking) taste perception, especially salty taste perception and as potential therapeutics. In addition, this invention relates to novel methods for identifying taste-specific genes that can be used as markers for different taste cell types, including sweet, bitter, umami, sour, salty, and other taste cells in mammals as well as assays that measure the activity of the sweet, bitter, umami, or sour receptor in the presence of these genes to identify modulators of sweet, bitter, umami, and sour taste and to identify therapeutics especially for treating digestive or metabolic disorders, taste loss, and oral infections. Particularly, the genes identified herein and antibodies or oligos thereto can be used as markers to identify and/or purify specific taste cells e.g., from taste cell suspensions by use of FACS or magnetic bead cell selection or other known cell purification and isolation procedures.

公开(公告)号：US20160061833A1

公开(公告)日：2016-03-03

申请号：US14824936

申请日：2015-08-12

Applicant: SENOMYX, INC.

Inventor： Bryan Moyer ,  Albert Zlotnik ,  Peter Hevezi ,  Hortensia Soto ,  Dalia Kalabat ,  Min Lu ,  Na Gao ,  Evan Carl White

IPC: G01N33/566 ,  G01N33/50 ,  C12Q1/68

CPC classification number: G01N33/566 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12Q1/6883 ,  C12Q2600/136 ,  C12Q2600/158 ,  C40B30/04 ,  C40B40/10 ,  G01N33/5005 ,  G01N33/5044 ,  G01N33/6872 ,  G01N2333/726 ,  G01N2500/10

Abstract: This invention relates to novel rationale and methods for identifying human and primate taste-specific genes, including genes involved in salty taste perception, especially human salty taste perception, but also genes involved in sweet, bitter, umami, and sour taste perception, and genes involved in other taste cell or taste receptor related activities such as digestive function and digestive related diseases, taste cell turnover, immunoregulation of the oral and digestive tract, and metabolic regulation such as in diabetes and obesity, the genes identified using these methods, and assays for identifying taste modulators (enhancers or blockers) and potential therapeutics using these genes. These compounds have potential application in modulating (enhancing or blocking) taste perception, especially salty taste perception and as potential therapeutics. In addition, this invention relates to novel methods for identifying taste-specific genes that can be used as markers for different taste cell types, including sweet, bitter, umami, sour, salty, and other taste cells in mammals as well as assays that measure the activity of the sweet, bitter, umami, or sour receptor in the presence of these genes to identify modulators of sweet, bitter, umami, and sour taste and to identify therapeutics especially for treating digestive or metabolic disorders, taste loss, and oral infections. Particularly, the genes identified herein and antibodies or oligos thereto can be used as markers to identify and/or purify specific taste cells e.g., from taste cell suspensions by use of FACS or magnetic bead cell selection or other known cell purification and isolation procedures.

Abstract translation: 本发明涉及用于鉴定人类和灵长类动物特异性基因的新的理论和方法，包括涉及咸味感知的基因，特别是人类咸味感知，以及参与甜味，苦味，鲜味和酸味感觉的基因以及基因 参与其他味觉细胞或味觉受体相关活性，如消化功能和消化相关疾病，味觉细胞周转，口服和消化道的免疫调节，以及代谢调节，如糖尿病和肥胖症，使用这些方法鉴定的基因和测定 用于鉴定使用这些基因的味觉调节剂（增强子或阻断剂）和潜在的治疗剂。 这些化合物具有潜在的应用于调节（增强或阻断）味觉，特别是咸味感和作为潜在的疗法。 此外，本发明涉及用于鉴定可用作哺乳动物中不同味道细胞类型（包括甜味，苦味，鲜味，酸味，咸味和其他味觉细胞）的标记的味道特异性基因的新方法，以及测量 在这些基因存在下甜味，苦味，鲜味或酸性受体的活性，以鉴定甜味，苦味，鲜味和酸味的调节剂，并鉴定治疗剂，特别是用于治疗消化或代谢紊乱，口味丧失和口腔感染 。 特别地，本文鉴定的基因和其抗体或寡核苷酸可以用作标识物，以通过使用FACS或磁珠细胞选择或其他已知的细胞纯化和分离程序来鉴定和/或纯化特定味细胞，例如来自味细胞悬浮液。

公开(公告)号：US10191050B2

公开(公告)日：2019-01-29

申请号：US15681755

申请日：2017-08-21

Applicant: SENOMYX, INC.

Inventor： Bryan Moyer ,  Albert Zlotnik ,  Peter Hevezi ,  Hortensia Soto ,  Dalia Kalabat ,  Min Lu ,  Na Gao ,  Evan Carl White

IPC: G01N33/566 ,  C12Q1/6883 ,  C40B30/04 ,  C40B40/10 ,  G01N33/50 ,  G01N33/68 ,  C12Q1/6806 ,  C12Q1/6876

Abstract: This invention relates to novel rationale and methods for identifying human and primate taste-specific genes, including genes involved in salty taste perception, especially human salty taste perception, but also genes involved in sweet, bitter, umami, and sour taste perception, and genes involved in other taste cell or taste receptor related activities such as digestive function and digestive related diseases, taste cell turnover, immunoregulation of the oral and digestive tract, and metabolic regulation such as in diabetes and obesity, the genes identified using these methods, and assays for identifying taste modulators (enhancers or blockers) and potential therapeutics using these genes. These compounds have potential application in modulating (enhancing or blocking) taste perception, especially salty taste perception and as potential therapeutics. In addition, this invention relates to novel methods for identifying taste-specific genes that can be used as markers for different taste cell types, including sweet, bitter, umami, sour, salty, and other taste cells in mammals as well as assays that measure the activity of the sweet, bitter, umami, or sour receptor in the presence of these genes to identify modulators of sweet, bitter, umami, and sour taste and to identify therapeutics especially for treating digestive or metabolic disorders, taste loss, and oral infections. Particularly, the genes identified herein and antibodies or oligos thereto can be used as markers to identify and/or purify specific taste cells e.g., from taste cell suspensions by use of FACS or magnetic bead cell selection or other known cell purification and isolation procedures.

公开(公告)号：US20140206004A1

公开(公告)日：2014-07-24

申请号：US14162313

申请日：2014-01-23

Applicant: SENOMYX INC.

Inventor： Bryan Moyer ,  Albert Zlotnik ,  Peter Hevezi ,  Hortensia Soto ,  Dalia Kalabat ,  Min Lu ,  Na Gao ,  Evan Carl White

IPC: C12Q1/68

CPC classification number: G01N33/566 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12Q1/6883 ,  C12Q2600/136 ,  C12Q2600/158 ,  C40B30/04 ,  C40B40/10 ,  G01N33/5005 ,  G01N33/5044 ,  G01N33/6872 ,  G01N2333/726 ,  G01N2500/10

Abstract: This invention relates to novel rationale and methods for identifying human and primate taste-specific genes, including genes involved in salty taste perception, especially human salty taste perception, but also genes involved in sweet, bitter, umami, and sour taste perception, and genes involved in other taste cell or taste receptor related activities such as digestive function and digestive related diseases, taste cell turnover, immunoregulation of the oral and digestive tract, and metabolic regulation such as in diabetes and obesity, the genes identified using these methods, and assays for identifying taste modulators (enhancers or blockers) and potential therapeutics using these genes. These compounds have potential application in modulating (enhancing or blocking) taste perception, especially salty taste perception and as potential therapeutics. In addition, this invention relates to novel methods for identifying taste-specific genes that can be used as markers for different taste cell types, including sweet, bitter, umami, sour, salty, and other taste cells in mammals as well as assays that measure the activity of the sweet, bitter, umami, or sour receptor in the presence of these genes to identify modulators of sweet, bitter, umami, and sour taste and to identify therapeutics especially for treating digestive or metabolic disorders, taste loss, and oral infections. Particularly, the genes identified herein and antibodies or oligos thereto can be used as markers to identify and/or purify specific taste cells e.g., from taste cell suspensions by use of FACS or magnetic bead cell selection or other known cell purification and isolation procedures.

Abstract translation: 本发明涉及用于鉴定人类和灵长类动物特异性基因的新的理论和方法，包括涉及咸味感知的基因，特别是人类咸味感知，以及参与甜味，苦味，鲜味和酸味感觉的基因以及基因 参与其他味觉细胞或味觉受体相关活性，如消化功能和消化相关疾病，味觉细胞周转，口服和消化道的免疫调节，以及代谢调节，如糖尿病和肥胖症，使用这些方法鉴定的基因和测定 用于鉴定使用这些基因的味觉调节剂（增强子或阻断剂）和潜在的治疗剂。 这些化合物具有潜在的应用于调节（增强或阻断）味觉，特别是咸味感和作为潜在的疗法。 此外，本发明涉及用于鉴定可用作哺乳动物中不同味道细胞类型（包括甜味，苦味，鲜味，酸味，咸味和其他味觉细胞）的标记的味道特异性基因的新方法，以及测量 在这些基因存在下甜味，苦味，鲜味或酸性受体的活性，以鉴定甜味，苦味，鲜味和酸味的调节剂，并鉴定治疗剂，特别是用于治疗消化或代谢紊乱，口味丧失和口腔感染 。 特别地，本文鉴定的基因和其抗体或寡核苷酸可以用作标识物，以通过使用FACS或磁珠细胞选择或其他已知的细胞纯化和分离程序来鉴定和/或纯化特定味细胞，例如来自味细胞悬浮液。

公开(公告)号：US09139867B2

公开(公告)日：2015-09-22

申请号：US14162313

申请日：2014-01-23

Applicant: SENOMYX, INC.

Inventor： Bryan Moyer ,  Albert Zlotnik ,  Peter Hevezi ,  Hortensia Soto ,  Dalia Kalabat ,  Min Lu ,  Na Gao ,  Evan Carl White

IPC: C12Q1/68 ,  C40B30/04 ,  C40B40/10 ,  G01N33/50 ,  G01N33/566 ,  G01N33/68

CPC classification number: G01N33/566 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12Q1/6883 ,  C12Q2600/136 ,  C12Q2600/158 ,  C40B30/04 ,  C40B40/10 ,  G01N33/5005 ,  G01N33/5044 ,  G01N33/6872 ,  G01N2333/726 ,  G01N2500/10

Abstract: This invention relates to novel rationale and methods for identifying human and primate taste-specific genes, including genes involved in salty taste perception, especially human salty taste perception, but also genes involved in sweet, bitter, umami, and sour taste perception, and genes involved in other taste cell or taste receptor related activities such as digestive function and digestive related diseases, taste cell turnover, immunoregulation of the oral and digestive tract, and metabolic regulation such as in diabetes and obesity, the genes identified using these methods, and assays for identifying taste modulators (enhancers or blockers) and potential therapeutics using these genes. These compounds have potential application in modulating (enhancing or blocking) taste perception, especially salty taste perception and as potential therapeutics. In addition, this invention relates to novel methods for identifying taste-specific genes that can be used as markers for different taste cell types, including sweet, bitter, umami, sour, salty, and other taste cells in mammals as well as assays that measure the activity of the sweet, bitter, umami, or sour receptor in the presence of these genes to identify modulators of sweet, bitter, umami, and sour taste and to identify therapeutics especially for treating digestive or metabolic disorders, taste loss, and oral infections. Particularly, the genes identified herein and antibodies or oligos thereto can be used as markers to identify and/or purify specific taste cells e.g., from taste cell suspensions by use of FACS or magnetic bead cell selection or other known cell purification and isolation procedures.

Abstract translation: 本发明涉及用于鉴定人类和灵长类动物特异性基因的新的理论和方法，包括涉及咸味感知的基因，特别是人类咸味感知，以及参与甜味，苦味，鲜味和酸味感觉的基因以及基因 参与其他味觉细胞或味觉受体相关活性，如消化功能和消化相关疾病，味觉细胞周转，口服和消化道的免疫调节，以及代谢调节，如糖尿病和肥胖症，使用这些方法鉴定的基因和测定 用于鉴定使用这些基因的味觉调节剂（增强子或阻断剂）和潜在的治疗剂。 这些化合物具有潜在的应用于调节（增强或阻断）味觉，特别是咸味感和作为潜在的疗法。 此外，本发明涉及用于鉴定可用作哺乳动物中不同味道细胞类型（包括甜味，苦味，鲜味，酸味，咸味和其他味觉细胞）的标记的味道特异性基因的新方法，以及测量 在这些基因存在下甜味，苦味，鲜味或酸性受体的活性，以鉴定甜味，苦味，鲜味和酸味的调节剂，并鉴定治疗剂，特别是用于治疗消化或代谢紊乱，口味丧失和口腔感染 。 特别地，本文鉴定的基因和其抗体或寡核苷酸可以用作标识物，以通过使用FACS或磁珠细胞选择或其他已知的细胞纯化和分离程序来鉴定和/或纯化特定味细胞，例如来自味细胞悬浮液。