公开(公告)号：US20170088804A1

公开(公告)日：2017-03-30

申请号：US15280139

申请日：2016-09-29

Applicant: SHIBUYA CORPORATION

Inventor： Kenji YONEDA ,  Ichiro KOSHIDA

IPC: C12M1/12

CPC classification number: C12M23/04 ,  C12M21/08 ,  C12M33/06

Abstract: An apparatus 1 for producing a cell mass sheet is structured so as to produce a cell mass sheet 4 in which a plurality of cell masses 2 that have been planarly arranged on a mounting surface 13a in a culture container 3 are fused to each other by being cultured.The apparatus includes guide means G that has a plurality of accommodating portions Ga which can accommodate the cell masses 2 therein, and suction nozzles 8 (supply means) that supply the cell masses 2 into the accommodating portions Ga of the guide means G, which are arranged on the mounting surface 13a; and is structured so that the suction nozzles 8 array the cell masses 2 on the mounting surface 13a, and after that, the guide means G retracts from the above the mounting surface 13a. The apparatus can produce a cell mass sheet that has a constant shape and also a uniform thickness.

公开(公告)号：US20170121674A1

公开(公告)日：2017-05-04

申请号：US15337785

申请日：2016-10-28

Applicant: SHIBUYA CORPORATION

Inventor： Kenji YONEDA ,  Ichiro KOSHIDA ,  Akira HATANAKA ,  Hiroyuki NAGAI

IPC: C12N5/00 ,  C12M3/04 ,  C12M1/26 ,  C12M1/00

CPC classification number: C12N5/0062 ,  C12M23/50 ,  C12M27/10 ,  C12M29/06 ,  C12M33/04 ,  C12M47/02 ,  C12N5/0068 ,  C12N2513/00 ,  C12N2535/10

Abstract: The present invention relates to a method for producing a cell mass structure, which produces a cell mass structure (adhesion pad 4) in which a plurality of cell aggregates 2 arranged inside a culture container 3 accommodating a culture liquid are fused to each other by being cultured. A plurality of pins 16 are erected inside the above-described culture container 3, the above-described cell aggregates 2 are accommodated by the plurality of pins 16 with parts between the plurality of pins 16 and pins 16 as accommodating portions H, and the cell aggregates 2 are cultured in a state of being accommodated in the plurality of adjacent accommodating portions H. It is possible to obtain a required shape and dimension, and the cell mass structure can be obtained without giving damage to cells further.