公开(公告)号：US20180079778A1

公开(公告)日：2018-03-22

申请号：US15569424

申请日：2016-04-28

Applicant: SHOWA DENKO K.K.

Inventor： Yuzuru KOKIDO ,  Natsuno MATSUI ,  Hirobumi AOKI ,  Tadashi YONEDA

IPC: C07K1/18 ,  A61K39/395 ,  C07K16/06

CPC classification number: C07K1/18 ,  A61K39/39591 ,  C07K16/00 ,  C07K16/065

Abstract: Provided is a method of removing a protein aggregate which can obtain a useful protein monomer in a high yield and a high purity as a raw material of a pharmaceutical product or the like. The method of removing a protein aggregate includes: a step of making the monomer of a protein and the aggregate of proteins adsorb to the column by making a solution containing a monomer of a protein and an aggregate of proteins pass through a column containing a hard porous polymer self-supporting structure to which a strong cation exchange group is fixed; and a step of making a mobile phase consisting of a mixed solution of a buffer solution and an ionic buffer solution pass through the column to which the monomer of a protein and the aggregate of proteins are adsorbed, to selectively elute the monomer of a protein.

公开(公告)号：US20180298052A1

公开(公告)日：2018-10-18

申请号：US15569466

申请日：2016-04-28

Applicant: SHOWA DENKO K.K.

Inventor： Natsuno MATSUI ,  Yuzuru KOKIDO ,  Hirobumi AOKI ,  Tadashi YONEDA

IPC: C07K1/18 ,  C07K1/14 ,  C07K1/22

Abstract: An object of the present invention is to obtain a protein monomer useful as a raw material for medicines industrially and economically in high yield and high purity. In the method for purifying a protein of the present invention, a protein solution containing a protein monomer and a protein aggregate is passed through a column holding a porous rigid polymeric self-supporting structure to which a hydrophobic group is immobilized, and then recovering the protein monomer in a flow-through mode.