公开(公告)号：US20250069384A1

公开(公告)日：2025-02-27

申请号：US18936019

申请日：2024-11-04

Applicant: Sphere Fluidics Limited

Inventor： Nicholas DAYRELL-ARMES ,  David HOLMES ,  Frank F. CRAIG ,  Marian REHAK ,  Dimitris JOSEPHIDES ,  Robert SALTER ,  William WHITLEY ,  Sinan GOKKAYA ,  Raphael RUIS

IPC: G06V10/82 ,  B01L3/00 ,  G01N15/10 ,  G01N15/14 ,  G01N15/1433 ,  G06F18/2431 ,  G06T7/70 ,  G06V10/44 ,  G06V10/764 ,  G06V20/69

Abstract: ABSTRACT:
An instrument for processing droplets in a microfluidic system. The instrument captures a time sequence of images of a droplet as it passes through a channel in a microfluidic system. The instrument also processes each image of the sequence of images using a convolutional neural network to count a number of cells or other entities visible in each image the droplet. This involves processing the count of the number of cells or other entities visible in each image of the droplet to determine an estimated number of cells or other entities in the droplet. The instrument controls a microfluidic process performed on the droplet, e.g. droplet dispensing, responsive to the estimated number of cells or other entities in the droplet. The instrument uses the changing orientation and disposition of droplet contents in combination with machine learning to improve monoclonality assurance.

公开(公告)号：US20200215546A1

公开(公告)日：2020-07-09

申请号：US16625021

申请日：2018-06-21

Applicant: SPHERE FLUIDICS LIMITED

Inventor： Dimitris JOSEPHIDES ,  Marian REHAK ,  David HOLMES ,  Frank F. Craig

IPC: B01L3/00

Abstract: This invention generally relates to methods and systems for dispensing a droplet, in particular a picolitre volume droplet containing one or more entities. We disclose herein a microfluidic droplet dispensing system comprising: a droplet feed line to carry droplets for dispensing; a dispensing line to carry dispensing fluid for dispensing the droplets; a droplet outlet line coupled to a droplet outlet; a first junction having a first input to receive a flow from said droplet feed line, having a second input to receive a flow from said dispensing line and having an output to said droplet outlet line; a waste line coupled to said droplet output line between said first junction and said droplet output; a first pressure source to provide pressurised dispensing fluid to said dispensing line to eject droplets from said droplet output; a droplet dispensing valve between said first pressure source and said dispensing line to control the ejection of droplets from said droplet output; wherein the system further comprises: a second pressure source to provide pressurised dispensing fluid to a droplet protect line; a second junction having a first input from said droplet feed line, having a second input from said droplet protect line, and having an output; and a volume flow throttle coupled between the output of said second junction and the first input of said first junction to restrict a volume flow from said second junction into said first junction.