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公开(公告)号：US20240417621A1
公开(公告)日：2024-12-19
申请号：US18818982
申请日：2024-08-29
Applicant: SPHERE FLUIDICS LIMITED
Inventor： Xin LI , Clive A. SMITH , Alexandra CLAY , Colin PILKINGTON
Abstract: A surfactant of formula (I): A-(L1)a-(CH2)b-(L2)c-X (I) wherein A is a perfluoropolyether; L1 is CONR′, wherein R′ is selected from H and C1-6 alkyl; a is 0 or 1; b is 0 or an integer between 1 and 10; L2 is a linking group; c is 0 or 1; and X is a charged group.
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公开(公告)号：US20230158502A1
公开(公告)日：2023-05-25
申请号：US17919398
申请日：2020-04-17
Applicant: SPHERE FLUIDICS LIMITED
Inventor： Dimitris Josephides , James LONG , Xin LI
IPC: B01L3/00
CPC classification number: B01L3/502784 , B01L2200/0636 , B01L2400/0487 , B01L2400/086 , B01L2300/0867
Abstract: We describe a microfluidic structure for spacing out droplets, the structure comprising: a main channel for guiding droplets in a spacing fluid; a first inlet for introducing droplets into the main channel; and a second inlet for introducing a spacing fluid into the main channel, wherein a cross-sectional area of the main channel decreases downstream from the first inlet. We also describe a method of spacing out droplets using a microfluidic structure.
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公开(公告)号：US10545083B2
公开(公告)日：2020-01-28
申请号：US15502630
申请日：2015-08-07
Applicant: Sphere Fluidics Limited
Inventor： Clive Adrian Smith , Xin Li , Frank F. Craig
Abstract: We describe a method comprising: providing a droplet comprising a plurality of constituents, splitting said droplet into a first droplet and a second droplet, wherein said first droplet comprises a first fraction of said plurality of constituents and said second droplet comprises a second fraction of said plurality of constituents, analysing said constituents of said first fraction of said plurality of constituents in said first droplet, and sorting said second droplet dependent on an outcome of said analysis.
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公开(公告)号：US20180133715A1
公开(公告)日：2018-05-17
申请号：US15578455
申请日：2016-06-03
Applicant: SPHERE FLUIDICS LIMITED
Inventor： Frank F. CRAIG , Marian REHAK , David HOLMES , Clive A. SMITH , Xin LIU , Giuseppe BENAZZI , Xin LI , Vinayaka PAWATE
CPC classification number: B01L3/502784 , B01L3/502761 , B01L2200/0652 , B01L2200/0673 , B01L2300/0627 , B01L2300/0864 , B01L2400/0487 , B01L2400/0644 , G01N15/1484 , G01N35/08 , G01N2015/1481 , G01N2015/149
Abstract: Instruments and cartridges for processing droplets in emulsions containing biological entities such as cells. A method of such processing comprises providing a plurality of the entities in a fluid; preparing a droplet from the fluid; determining whether the droplet contains one or more entities of said plurality of entities, or whether said droplet does not contain a said entity; sorting said droplet dependent on an outcome of the determination; and dispensing the sorted droplet into a reservoir. The dispensing may comprise identifying and extracting the sorted droplet from a first fluidic flow path of said fluid by transferring the sorted droplet from into a second fluidic flow path and then ejecting the sorted droplet into a reservoir by applying pressure to the second fluidic flow path. The droplet contents may be tracked so that the contents of an individual droplet can be sorted, selectively dispensed, and retrieved.
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公开(公告)号：US10828640B2
公开(公告)日：2020-11-10
申请号：US15754754
申请日：2016-09-06
Applicant: SPHERE FLUIDICS LIMITED
Inventor： Xin Li , Xin Liu , Alexandra Clay
Abstract: A microfluidic structure for spacing out and aligning entities in an aqueous suspension is provided. The structure comprises: a channel for guiding entities in an aqueous suspension; a first comb of first inlets arranged on a first side of the channel for introducing a spacing medium into the channel; and a second comb of second inlets arranged on a second side of the channel for introducing the spacing medium into the channel; wherein the first side is opposite the second side, and wherein one of the first inlets has a corresponding, respective one of the second inlets at a substantially similar longitudinal position along the channel.
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Patent Agency Ranking
公开(公告)号：US20200316545A1
公开(公告)日：2020-10-08
申请号：US16768121
申请日：2018-11-29
Applicant: SPHERE FLUIDICS LIMITED
Inventor： Xin LI , Clive A. SMITH , Alexandra CLAY , Colin PILKINGTON
Abstract: A surfactant of formula (I): A-(L1)a-(C2)b-(L2)c-X (I) whereinA is a perfluoropolyether;L1 is CONR', wherein R′ is selected from H and C1-6 alkyl;a is 0 or 1;b is 0 or an integer between 1 and 10;L2 is a linking group;c is 0 or 1; andX is a charged group.
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公开(公告)号：US20200017635A1
公开(公告)日：2020-01-16
申请号：US16303769
申请日：2017-05-26
Applicant: SPHERE FLUIDICS LIMITED
Inventor： Xin LI , Olive A Smith , Alexandra Clay , Frank F Craig
IPC: C08G65/332 , C08G65/00 , C08G65/333 , C08G65/334 , C12Q1/6806 , B01F17/00
Abstract: The present invention provides a surfactant having a formula selected from the group consisting of: B—((X)x—(CH2)a-Mn(VI), (A-(CH2)—(X)x—B—(X)x—(CH2)a-A)n (IV), (A-(CH2)a—(X)x—B)n (V), and (B)n—(X)x—(CH2)a—A (VII), wherein A is a perfluoropolyether; a is a positive integer; X is either a covalent bond or a linking group; x is a positive integer; B is a polyalkylene oxide unit; n is a positive integer greater than 1 and, in compounds comprising more than one A, B, X, a and x, each may be the same or different. The present invention also relates to methods of making such surfactants, uses of such surfactants and emulsions comprising such surfactants.
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公开(公告)号：US10247655B2
公开(公告)日：2019-04-02
申请号：US14907867
申请日：2014-07-30
Applicant: Sphere Fluidics Limited
Inventor： Clive A. Smith , Xin Li , Karine Enesa , Xin Liu , Frank F. Craig , Graeme Whyte
Abstract: A method of label-free cell or particle sorting in a microfluidic device includes providing a stream of aqueous droplets in oil in a channel of the microfluidic device, wherein at least some of the droplets include cells or particles and illuminating the stream from a first direction. Then, one detects scattered light from cells or particles within said aqueous droplets in a second direction. Next, one determines a number of the cells or particles in each droplet from the scattered light and sorts the aqueous droplets into differentiated streams. Notably, the refractive index of said oil is modified to closely match a refractive index of the aqueous droplets to reduce light scattered from boundaries of the droplets. A volume of the droplets can also controlled.
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公开(公告)号：US20160169788A1
公开(公告)日：2016-06-16
申请号：US14907936
申请日：2014-07-30
Applicant: SPHERE FLUIDICS LIMITED
Inventor： Clive A. SMITH , Xin Li , Karing ENESA , Xin LIU , Frank F. CRAIG , Graeme WHYTE
IPC: G01N15/14
CPC classification number: G01N15/1484 , B01L3/502784 , B01L2200/0652 , B01L2200/0673 , G01N2015/1481 , G01N2015/149
Abstract: The present invention provides an emulsion for sorting droplets in a microfluidic device, the emulsion comprising: a discontinuous aqueous phase; and a continuous oil phase; wherein said aqueous phase comprises at least one analyte, said oil phase comprises a fluorous oil and a refractive index modifying compound comprising at least one aromatic ring, and the refractive index of said aqueous phase and the refractive index of said oil phase are substantially matched.
Abstract translation: 本发明提供了一种用于在微流体装置中分选液滴的乳液，所述乳液包括：不连续的水相; 和连续油相; 其中所述水相包含至少一种分析物，所述油相包括含氟油和包含至少一个芳环的折射率改性化合物，并且所述水相的折射率和所述油相的折射率基本匹配。
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公开(公告)号：US20250069384A1
公开(公告)日：2025-02-27
申请号：US18936019
申请日：2024-11-04
Applicant: Sphere Fluidics Limited
Inventor： Nicholas DAYRELL-ARMES , David HOLMES , Frank F. CRAIG , Marian REHAK , Dimitris JOSEPHIDES , Robert SALTER , William WHITLEY , Sinan GOKKAYA , Raphael RUIS
IPC: G06V10/82 , B01L3/00 , G01N15/10 , G01N15/14 , G01N15/1433 , G06F18/2431 , G06T7/70 , G06V10/44 , G06V10/764 , G06V20/69
Abstract: ABSTRACT:
An instrument for processing droplets in a microfluidic system. The instrument captures a time sequence of images of a droplet as it passes through a channel in a microfluidic system. The instrument also processes each image of the sequence of images using a convolutional neural network to count a number of cells or other entities visible in each image the droplet. This involves processing the count of the number of cells or other entities visible in each image of the droplet to determine an estimated number of cells or other entities in the droplet. The instrument controls a microfluidic process performed on the droplet, e.g. droplet dispensing, responsive to the estimated number of cells or other entities in the droplet. The instrument uses the changing orientation and disposition of droplet contents in combination with machine learning to improve monoclonality assurance.
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