公开(公告)号：US20170183663A1

公开(公告)日：2017-06-29

申请号：US15395233

申请日：2016-12-30

Applicant: STC.UNM

Inventor： Anatoliy Markiv ,  Ravi Venkata Durvasula ,  Angray Singh Kang

IPC: C12N15/62 ,  C12N15/63

CPC classification number: C12N15/62 ,  A61K49/0045 ,  A61K49/0058 ,  A61N5/062 ,  C07K14/001 ,  C07K16/18 ,  C07K16/30 ,  C07K16/32 ,  C07K2317/62 ,  C07K2317/622 ,  C07K2319/00 ,  C07K2319/60 ,  C12N15/63 ,  C12Q1/04 ,  G01N33/53 ,  G01N33/582

Abstract: Embodiments of the present invention provide for the facile generation of a stable recombinant fusion polypeptides with intrinsic fluorescent properties. The recombinant antibodies may be suitable for qualitative and/or quantitative immunofluorescence analysis. Generally, the fluorescent polypeptides include a fluorescent domain comprising a C-terminus and an N-terminus; a first antibody domain covalently linked to the C-terminus; and a second antibody domain covalently linked to the N-terminus.

公开(公告)号：US20150299646A1

公开(公告)日：2015-10-22

申请号：US14698481

申请日：2015-04-28

Applicant: STC.UNM

Inventor： Ravi Venkata Durvasula ,  Subba Rao Durvasula ,  Annabeth Fieck ,  Ivy Hurwitz

IPC: C12N1/12 ,  C02F3/32 ,  A01N65/03

CPC classification number: C12N1/12 ,  A01N65/03 ,  C02F3/322 ,  C02F3/325 ,  C02F3/348 ,  C02F2301/10 ,  C02F2305/06 ,  C12R1/89

Abstract: Provided herein is an extremophile green alga designated as Scenedesmus species Novo, from Jemez warm water springs, New Mexico. Sequencing 18S rDNA confirmed the alga as a new species. It is capable of producing high levels of microalgal biomass in wastewater under harsh ambient climatic conditions, and of yielding high levels of lipids and carotenes. Cultures in TAP medium at 24±1° C. at continuous light (132-148 μmol photons m−2s−1) attained peak biomass levels 27.4×106 cells ml−1, 49.11 μg ml−1 chlorophyll α, 24.93 μg ml−1 carotene on the seventh day and a division rate of 0.54 day−1. High levels of biomass were sustained in sterilized and unsterilized municipal wastewater, enriched with 1% TAP nutrients or unenriched. The microalga is useful in the production of biofuels, fertilizers, dietary nutrients, pharmaceuticals, polymers, biofilters to remove nutrients and other pollutants from wastewaters, in space technology, and laboratory research systems.

Abstract translation: 这里提供的是一种极端微生物绿藻，被称为Scenedesmus物种Novo，来自Jemez温泉泉，新墨西哥州。 测序18S rDNA确认藻类为新物种。 它能够在恶劣的环境气候条件下在废水中产生高水平的微藻生物质，并产生高水平的脂质和胡萝卜素。 在24±1℃的TAP培养基中连续培养（132-148μmol光子m-2s-1）达到峰值生物量27.4×106个细胞ml-1,49.11μg/ ml的叶绿素α，24.93μg/ 1胡萝卜素，分化率为0.54天-1。 消毒和未灭菌的城市废水中维持高水平的生物量，富含1％TAP营养或未富集。 微藻可用于生物燃料，肥料，膳食营养素，药物，聚合物，生物过滤器，以从废水，空间技术和实验室研究系统中去除营养物质和其他污染物。