公开(公告)号：US20230160024A1

公开(公告)日：2023-05-25

申请号：US17531313

申请日：2021-11-19

Applicant: STMICROELECTRONICS S.r.l.

Inventor： Massimiliano PESATURO ,  Lillo RAIA ,  Salvatore PETRALIA ,  Domenico GIUSTI ,  Marco FERRERA

IPC: C12Q1/70 ,  C12Q1/686 ,  C12Q1/6806 ,  B01L3/00 ,  B01L7/00 ,  B41M5/00

CPC classification number: C12Q1/701 ,  C12Q1/686 ,  C12Q1/6806 ,  B01L3/527 ,  B01L7/52 ,  B41M5/0023 ,  B01L2200/0647 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/0819 ,  B01L2300/16

Abstract: A substrate has a plurality of microdots positioned thereon. Each microdot contains one or more primers for gene amplification for a particular target gene. The microdots are placed on the substrate and the substrate is positioned in a housing. The housing has a sample fluid to be tested introduced therein covering the microdot array. While the sample fluid is overlying the substrate, the amplification of the target gene is carried out if it is present within the sample. If the target gene that matches the primers is not present, then amplification will not take place. The fluid also contains fluorophores which will be fixed into the gene as it increases in size as it clearly detects if gene amplification has occurred by detecting the amount of light detected for a particular microdot. In a preferred embodiment, the sample fluid is placed on top of a sealing layer that is less dense then water, such as wax or mineral oil. During a heating of the sample fluid and sealing layer, the sample fluid will sink to the bottom of the sealing layer so that it is fully encased and protected.