公开(公告)号：US20170362650A1

公开(公告)日：2017-12-21

申请号：US15536107

申请日：2015-12-18

Applicant: STOWERS INSTITUTE FOR MEDICAL RESEARCH

Inventor： Julia Zeitlinger ,  Qiye He ,  Jeff Johnston

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q2535/122 ,  C12Q2537/159 ,  C12Q2563/179

Abstract: The present invention provides, inter alia, methods and kits for identifying where a polypeptide of interest binds in a genome. The methods include a) carrying out a chromatin immunoprecipitation coupled to exonuclease digestion (ChIP-exo) process with an antibody against the polypeptide of interest; (b) extracting a polynucleotide fragment to which the polypeptide of interest binds; (c) carrying out a library preparation protocol adapted from an individual nucleotide resolution UV cross-linking and immunoprecipitation (iCLIP) process on the ChIP-exo processed polynucleotide fragment; and (d) sequencing the resulting polynucleotides. The kits include: (a) reagents sufficient to carry out ChIP-exo; (b) reagents sufficient to carry out the library preparation protocol adapted from the iCLIP process; and (c) instructions for use.