公开(公告)号：US20220228194A1

公开(公告)日：2022-07-21

申请号：US17575924

申请日：2022-01-14

Applicant: SYSMEX CORPORATION

Inventor： Masahiro MIURA ,  Kenji AKAMA ,  Masaaki KAWAI ,  Masaya OKADA ,  Kentaro SHIRAI ,  Yuichiro YOSHIDA ,  Tasuku YOTORIYAMA ,  Michitaka NOTOYA ,  Soichi OUE

IPC: C12Q1/686

Abstract: In an example of an embodiment, a first sample set is prepared that includes a test sample prepared from a first subject sample and a reagent, and at least one control sample prepared from at least one of a positive control and a negative control, and a second sample set is prepared that includes a test sample prepared from a second subject sample and a reagent and does not contain at least one of the control samples contained in the first sample set. The nucleic acid amplification in the first sample set is measured in the first unit, the nucleic acid amplification in the second sample set is measured in the second unit, and the measurement result of each test sample contained in the first sample set and second sample set is analyzed based on the measurement result of the control sample contained in at least the first sample set.

公开(公告)号：US20240424495A1

公开(公告)日：2024-12-26

申请号：US18748459

申请日：2024-06-20

Applicant: SYSMEX CORPORATION

Inventor： Taiga AJIRI ,  Kentaro SHIRAI ,  Shingo FUJIYAMA ,  Hiroyuki OBINATA ,  Natsuki MAEDA

IPC: B01L3/00 ,  B01D29/01 ,  B01D29/90

Abstract: A particle separation chip that separates particles contained in a specimen based on particle size may include a substrate comprising a micro flow path inside of the substrate; and a perforated sheet comprising holes. The micro flow path includes: a main flow path including an inlet arranged at upstream side, into which a specimen comprising a first particle, and a second particle that is smaller than the first particle is supplied; and a first outlet arranged at downstream side, from which a specimen comprising the first particle is discharged; and a branch flow path connected to the main flow path at branches along a flow direction of the main flow path, including a second outlet from which a specimen comprising the second particle is discharged at the downstream side. The main flow path and the branch flow path are connected via the holes of the perforated sheet at the branches.

公开(公告)号：US20210164886A1

公开(公告)日：2021-06-03

申请号：US17105013

申请日：2020-11-25

Applicant: SYSMEX CORPORATION

Inventor： Kentaro SHIRAI ,  Eri KATSUMATA ,  Yuji OKAMOTO ,  Momoko IMAKUBO ,  Jianyin LU

IPC: G01N15/14 ,  G02B21/26 ,  G02B21/36 ,  G01N21/64 ,  G06T7/62 ,  G06T7/50

Abstract: A cell analysis method, a cell analysis device, a cell analysis system, a cell analysis program, and a trained artificial intelligence algorithm generation method, generation device, and generation program to facilitate high-accuracy and high-speed analysis of more cells in a sample are provided. A cell analysis method for analyzing cells using an artificial intelligence algorithm, in which a sample containing cells is caused to flow in a flow path, an analysis target image is generated by imaging cells passing through the flow path, analysis data are generated from the generated analysis target image, the generated analysis data are input to the artificial intelligence algorithm, and data indicating the properties of the cells contained in the analysis target image are generated by the artificial intelligence algorithm.

公开(公告)号：US20210164883A1

公开(公告)日：2021-06-03

申请号：US17105047

申请日：2020-11-25

Applicant: SYSMEX CORPORATION

Inventor： Momoko IMAKUBO ,  Jianyin LU ,  Kentaro SHIRAI ,  Eri KATSUMATA ,  Yuji OKAMOTO

IPC: G01N15/14 ,  G06T7/00

Abstract: The present invention is to facilitate analysis of a plurality of analysis items. A cell analysis method for analyzing cells is provided in which data for analysis of cells contained in a sample are generated, and an artificial intelligence algorithm to be the input destination of the generated analysis data is selected from among a plurality of artificial intelligence algorithms, data indicating the properties of the cells are generated based on the analysis data via the artificial intelligence algorithm.

公开(公告)号：US20170370920A1

公开(公告)日：2017-12-28

申请号：US15695426

申请日：2017-09-05

Applicant: SYSMEX CORPORATION

Inventor： Kenji AKAMA ,  Seigo SUZUKI ,  Kenta ODA ,  Kentaro SHIRAI ,  Hana KUMAMOTO

IPC: G01N33/543 ,  G01N33/533 ,  G01N21/64

CPC classification number: G01N33/54306 ,  G01N15/14 ,  G01N21/6428 ,  G01N21/78 ,  G01N33/533 ,  G01N33/543 ,  G01N33/54313 ,  G01N2021/6439

Abstract: The present invention relates to a method for detecting an analyte in a sample, comprising the steps of: forming on each of carrier particles a complex containing a first capture substance capable of binding to an analyte, one molecule of the analyte, a second capture substance capable of binding to the analyte, and a catalyst; immobilizing a reaction product on each of the carrier particles by reacting the catalyst in the compolex with a substrate; and detecting the analyte by detecting the carrier particles on each of which the reaction product is immobilized.

公开(公告)号：US20200264181A1

公开(公告)日：2020-08-20

申请号：US16793073

申请日：2020-02-18

Applicant: SYSMEX CORPORATION

Inventor： Yusuke TAKAHASHI ,  Masatoshi YANAGIDA ,  Kentaro SHIRAI ,  Yuichi IJIRI ,  Shigeki IWANAGA

IPC: G01N33/574 ,  G01N15/14 ,  G01N21/64

Abstract: The present invention provides a method for determining a success or failure of a measurement of a target particle contained in a measurement sample. The method includes: detecting the target particle and other particle other than the target particle in the measurement sample; and determining the success or failure of the measurement of the target particle based on at least a detection result of the other particle.

公开(公告)号：US20200173980A1

公开(公告)日：2020-06-04

申请号：US16697270

申请日：2019-11-27

Applicant: SYSMEX CORPORATION

Inventor： Yusuke TAKAHASHI ,  Kentaro SHIRAI ,  Masatoshi YANAGIDA ,  Shigeki IWANAGA ,  Yuichi IJIRI ,  Keiko YOSHIKAWA

IPC: G01N33/49 ,  B01L3/00

Abstract: A method of determining a quality of a cell separation, according to an aspect, includes: separating, by running a specimen containing a first particle and a second particle in a flow path, the specimen to a first specimen containing the first particle and a second specimen containing the second particle, based on a difference in magnitude of a force exerted on each particle contained in the specimen; measuring first information on an amount of the first specimen and second information on an amount of the second specimen; and determining a quality of the separating based on the first information and the second information.

公开(公告)号：US20190128793A1

公开(公告)日：2019-05-02

申请号：US16177671

申请日：2018-11-01

Applicant: SYSMEX CORPORATION

Inventor： Kentaro SHIRAI ,  Yusuke TAKAHASHI ,  Masatoshi YANAGIDA ,  Shigeki IWANAGA ,  Tomokazu YOSHIDA ,  Yuichi IJIRI

IPC: G01N15/14 ,  G06T7/00 ,  G06T7/70

Abstract: A cell detection method and a cell detection system capable of performing target cell detection satisfactorily without interference and reducing the time required for detection of target cells are provided. The cell detection method includes a separation step of step S1 to obtain a sample containing target cells by removing at least a part of the non-target cells from a biological sample including target cells and non-target cells based on a difference in forces acting on target cells and non-target cells; a concentration step of step S3 of obtaining a measurement sample having a target cell concentration increased from the sample containing target cells; a detection of step S4 of detecting target cells by subjecting the measurement sample to an imaging flow cytometer.