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    Variants of glycoside hydrolases
    3.
    发明授权
    Variants of glycoside hydrolases 有权
    糖苷水解酶的变体

    公开(公告)号：US08383385B2

    公开(公告)日：2013-02-26

    申请号：US13009524

    申请日：2011-01-19

    申请人： Sarah Teter ,  Connie Ward ,  Joel Cherry ,  Aubrey Jones ,  Paul Harris ,  Jung Yi

    发明人： Sarah Teter ,  Connie Ward ,  Joel Cherry ,  Aubrey Jones ,  Paul Harris ,  Jung Yi

    IPC分类号： C12N9/42 ,  C12S3/00 ,  C12S3/02 ,  C12P19/14 ,  C11D3/386

    CPC分类号： C12N9/2437 ,  C11D3/38645 ,  C12N9/2434 ,  C12N9/2445 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01021 ,  C12Y302/01091 ,  C12Y302/01176

    摘要： The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.

    摘要翻译： 本发明涉及母体糖苷水解酶的变体，其包含在对应于位置21,94,157,205,206,247,337,350,373,383,438,455,467的一个或多个位置的取代，和 486，SEQ ID NO：2的氨基酸1至513，并且任选地还包含在对应于位置8,22,41,49,57,113,193,196,226,227,246,29的一个或多个位置的取代， SEQ ID NO：2的氨基酸1至513的251,255,259,301,356,371,411和462在对应于位置8,22,41,49,57,113， SEQ ID NO：2的氨基酸1至513的193,196,226,227,246,251,255,259,301,356,371,411和462，其中所述变体具有糖苷水解酶活性。 本发明还涉及编码变体糖苷水解酶的核苷酸序列以及包含核苷酸序列的核酸构建体，载体和宿主细胞。

    Variants of glycoside hydrolases
    4.
    发明授权
    Variants of glycoside hydrolases 有权
    糖苷水解酶的变体

    公开(公告)号：US07932073B2

    公开(公告)日：2011-04-26

    申请号：US11891249

    申请日：2007-08-08

    申请人： Sarah Teter ,  Connie Ward ,  Joel Cherry ,  Aubrey Jones ,  Paul Harris ,  Jung Yi

    发明人： Sarah Teter ,  Connie Ward ,  Joel Cherry ,  Aubrey Jones ,  Paul Harris ,  Jung Yi

    IPC分类号： C12N9/42 ,  C12S3/00 ,  C11D3/00

    CPC分类号： C12N9/2437 ,  C11D3/38645 ,  C12N9/2434 ,  C12N9/2445 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01021 ,  C12Y302/01091 ,  C12Y302/01176

    摘要： The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.

    摘要翻译： 本发明涉及母体糖苷水解酶的变体，其包含在对应于位置21,94,157,205,206,247,337,350,373,383,438,455,467的一个或多个位置的取代，和 486，SEQ ID NO：2的氨基酸1至513，并且任选地还包含在对应于位置8,22,41,49,57,113,193,196,226,227,246,29的一个或多个位置的取代， SEQ ID NO：2的氨基酸1至513的251,255,259,301,356,371,411和462在对应于位置8,22,41,49,57,113， SEQ ID NO：2的氨基酸1至513的193,196,226,227,246,251,255,259,301,356,371,411和462，其中所述变体具有糖苷水解酶活性。 本发明还涉及编码变体糖苷水解酶的核苷酸序列以及包含核苷酸序列的核酸构建体，载体和宿主细胞。

    Variants of glycoside hydrolases
    5.
    发明申请
    Variants of glycoside hydrolases 审中-公开
    糖苷水解酶的变体

    公开(公告)号：US20050048619A1

    公开(公告)日：2005-03-03

    申请号：US10926223

    申请日：2004-08-25

    申请人： Sarah Teter ,  Joel Cherry ,  Connie Ward ,  Aubrey Jones ,  Paul Harris ,  Jung Yi

    发明人： Sarah Teter ,  Joel Cherry ,  Connie Ward ,  Aubrey Jones ,  Paul Harris ,  Jung Yi

    IPC分类号： C12N9/42 ,  C07H21/04 ,  C12N9/24 ,  C12P21/04

    CPC分类号： C12N9/2437 ,  C11D3/38645 ,  C12N9/2434 ,  C12N9/2445 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01021 ,  C12Y302/01091 ,  C12Y302/01176 ,  Y02E50/16

    摘要： The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.

    摘要翻译： 本发明涉及母体糖苷水解酶的变体，其包含在对应于位置21,94,157,205,206,247,337,350,373,383,438,455,467的一个或多个位置的取代，和 486，SEQ ID NO：2的氨基酸1至513，并且任选地还包含在对应于位置8,22,41,49,57,113,193,196,226,227,246,29的一个或多个位置的取代， SEQ ID NO：2的氨基酸1至513的251,255,259,301,356,371,411和462在对应于位置8,22,41,49,57,113， SEQ ID NO：2的氨基酸1至513的193,196,226,227,246,251,255,259,301,356,371,411和462，其中所述变体具有糖苷水解酶活性。 本发明还涉及编码变体糖苷水解酶的核苷酸序列以及包含核苷酸序列的核酸构建体，载体和宿主细胞。

    Methods for transforming and expression screening of filamentous fungal cells with a DNA library
    6.
    发明授权
    Methods for transforming and expression screening of filamentous fungal cells with a DNA library 有权
    用DNA文库转化和表达筛选丝状真菌细胞的方法

    公开(公告)号：US09045748B2

    公开(公告)日：2015-06-02

    申请号：US11140150

    申请日：2005-05-26

    申请人： Sarah Teter ,  Michael Lamsa ,  Joel Cherry ,  Connie Ward

    发明人： Sarah Teter ,  Michael Lamsa ,  Joel Cherry ,  Connie Ward

    IPC分类号： C12N15/10 ,  C12N15/80 ,  C40B40/02

    CPC分类号： C12N15/1037 ,  C12N15/1086 ,  C12N15/80 ,  C40B40/02

    摘要： The present invention relates to methods for expression screening of filamentous fungal transformants, comprising: (a) isolating single colony transformants of a DNA library introduced into E. coli; (b) preparing DNA from each of the single colony E. coli transformants; (c) introducing a sample of each of the DNA preparations of step (b) into separate suspensions of protoplasts of a filamentous fungus to obtain transformants thereof, wherein each transformant contains one or more copies of an individual polynucleotide from the DNA library; (d) growing the individual filamentous fungal transformants of step (c) on selective growth medium, thereby permitting growth of the filamentous fungal transformants, while suppressing growth of untransformed filamentous fungi; and (e) measuring activity or a property of each polypeptide encoded by the individual polynucleotides. The present invention also relates to isolated polynucleotides encoding polypeptides of interest obtained by such methods, to nucleic acid constructs, expression vectors, and recombinant host cells comprising the isolated polynucleotides, and to methods of producing the polypeptides encoded by the isolated polynucleotides.

    摘要翻译： 本发明涉及丝状真菌转化体的表达筛选方法，其包括：（a）分离导入大肠杆菌的DNA文库的单个菌落转化体; （b）从每个单个菌落的大肠杆菌转化体制备DNA; （c）将步骤（b）的每种DNA制剂的样品引入到丝状真菌的原生质体的分离悬浮液中以获得其转化体，其中每个转化体含有来自DNA文库的单个多核苷酸的一个或多个拷贝; （d）在选择性生长培养基上生长步骤（c）的单个丝状真菌转化体，从而允许丝状真菌转化体生长，同时抑制未转化的丝状真菌的生长; 和（e）测量由各个多核苷酸编码的每种多肽的活性或性质。 本发明还涉及通过这些方法获得的编码感兴趣的多肽的分离的多核苷酸，包括分离的多核苷酸的核酸构建体，表达载体和重组宿主细胞，以及由分离的多核苷酸编码的多肽的制备方法。

    Variants of glycoside hydrolases
    8.
    发明申请
    Variants of glycoside hydrolases 有权
    糖苷水解酶的变体

    公开(公告)号：US20080167214A1

    公开(公告)日：2008-07-10

    申请号：US11891249

    申请日：2007-08-08

    申请人： Sarah Teter ,  Joel Cherry ,  Connie Ward ,  Aubrey Jones ,  Paul Harris ,  Jung Yi

    发明人： Sarah Teter ,  Joel Cherry ,  Connie Ward ,  Aubrey Jones ,  Paul Harris ,  Jung Yi

    IPC分类号： C11D3/386 ,  C12N9/24 ,  C07H21/04 ,  C12N15/63 ,  C12N5/06 ,  C12P21/02 ,  B09C1/10

    CPC分类号： C12N9/2437 ,  C11D3/38645 ,  C12N9/2434 ,  C12N9/2445 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01021 ,  C12Y302/01091 ,  C12Y302/01176

    摘要： The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.

    摘要翻译： 本发明涉及母体糖苷水解酶的变体，其包含在对应于位置21,94,157,205,206,247,337,350,373,383,438,455,467的一个或多个位置的取代，和 486，SEQ ID NO：2的氨基酸1至513，并且任选地还包含在对应于位置8,22,41,49,57,113,193,196,226,227,246,29的一个或多个位置的取代， SEQ ID NO：2的氨基酸1至513的251,255,259,301,356,371,411和462在对应于位置8,22,41,49,57,113， SEQ ID NO：2的氨基酸1至513的193,196,226,227,246,251,255,259,301,356,371,411和462，其中所述变体具有糖苷水解酶活性。 本发明还涉及编码变体糖苷水解酶的核苷酸序列以及包含核苷酸序列的核酸构建体，载体和宿主细胞。

    Methods for transforming and expression screening of filamentous fungal cells with a DNA library
    9.
    发明申请
    Methods for transforming and expression screening of filamentous fungal cells with a DNA library 有权

    公开(公告)号：US20060172379A1

    公开(公告)日：2006-08-03

    申请号：US11140150

    申请日：2005-05-26

    申请人： Sarah Teter ,  Michael Lamsa ,  Joel Cherry ,  Connie Ward

    发明人： Sarah Teter ,  Michael Lamsa ,  Joel Cherry ,  Connie Ward

    IPC分类号： C12P21/06 ,  C12N1/16 ,  C12N15/74

    CPC分类号： C12N15/1037 ,  C12N15/1086 ,  C12N15/80 ,  C40B40/02

    摘要： The present invention relates to methods for expression screening of filamentous fungal transformants, comprising: (a) isolating single colony transformants of a DNA library introduced into E. coli; (b) preparing DNA from each of the single colony E. coli transformants; (c) introducing a sample of each of the DNA preparations of step (b) into separate suspensions of protoplasts of a filamentous fungus to obtain transformants thereof, wherein each transformant contains one or more copies of an individual polynucleotide from the DNA library; (d) growing the individual filamentous fungal transformants of step (c) on selective growth medium, thereby permitting growth of the filamentous fungal transformants, while suppressing growth of untransformed filamentous fungi; and (e) measuring activity or a property of each polypeptide encoded by the individual polynucleotides. The present invention also relates to isolated polynucleotides encoding polypeptides of interest obtained by such methods, to nucleic acid constructs, expression vectors, and recombinant host cells comprising the isolated polynucleotides, and to methods of producing the polypeptides encoded by the isolated polynucleotides.

    Method for preparing maltogenic alpha-amylase variants
    10.
    发明申请
    Method for preparing maltogenic alpha-amylase variants 有权
    麦芽糖α-淀粉酶变体的制备方法

    公开(公告)号：US20110159570A1

    公开(公告)日：2011-06-30

    申请号：US13010219

    申请日：2011-01-20

    申请人： Joel Cherry ,  Allan Svendsen ,  Carsten Andersen ,  Lars Beier ,  Torben Peter Frandsen

    发明人： Joel Cherry ,  Allan Svendsen ,  Carsten Andersen ,  Lars Beier ,  Torben Peter Frandsen

    IPC分类号： C12N9/28

    CPC分类号： C12N9/2417 ,  A21D2/26 ,  A21D8/042

    摘要： The inventors have modified the amino acid sequence of a maltogenic alpha-amylase to obtain variants with improved properties, based on the three-dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties, e.g. an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.

    摘要翻译： 基于麦芽糖α-淀粉酶Novamyl的三维结构，本发明人已经修饰了产麦芽糖α-淀粉酶的氨基酸序列以获得具有改进性质的变体。 这些变体具有改变的物理化学性质，例如 改变的pH最佳，改善的热稳定性，增加的比活性，改变的切割模式或增加降低淀粉退化或面包陈化的能力。