公开(公告)号：US6162636A

公开(公告)日：2000-12-19

申请号：US384435

申请日：1999-08-27

申请人： Sang Yong Kim ,  Deok Kun Oh ,  Soo Ryun Jung

发明人： Sang Yong Kim ,  Deok Kun Oh ,  Soo Ryun Jung

IPC分类号： C12P19/02 ,  C12N1/14 ,  C12P7/18

CPC分类号： C12R1/645 ,  C12P7/18 ,  Y10S435/911

摘要： The present invention relates to a fermentation process for preparing erythritol with high productivity with novel mutant of Trigonopsis variabilis, more specifically, for preparing erythritol under optimal fermentation conditions for maximum erythritol production by optimizing the environmental conditions of culture such as pH, temperature and controlling osmotic pressure. A two-stage fermentation was performed to control osmotic pressure. Osmotic pressure was adjusted to a low level during growth phase and to a high level during production phase by adding glucose and NaCl. Therefore, erythritol production could be increased due to the increased mutant cells.

摘要翻译： 本发明涉及一种具有高生产能力的赤藓糖醇的发酵方法，其具有新颖的变异突变体，更具体地说，用于通过优化培养物的环境条件（如pH，温度和控制渗透压）在最佳发酵条件下最佳发酵条件下制备赤藓糖醇 压力。 进行两阶段发酵以控制渗透压。 通过添加葡萄糖和NaCl，渗透压在生长阶段调整到低水平，在生产阶段调整到高水平。 因此，由于增加的突变体细胞，赤藓糖醇的产生可能增加。

公开(公告)号：US6001616A

公开(公告)日：1999-12-14

申请号：US154218

申请日：1998-09-16

申请人： Sang Yong Kim ,  Deok Kun Oh ,  Soo Ryun Jung

发明人： Sang Yong Kim ,  Deok Kun Oh ,  Soo Ryun Jung

IPC分类号： C12P7/18 ,  C12N1/16 ,  C12P7/02

CPC分类号： C12R1/84 ,  C12P7/18 ,  Y10S435/938

摘要： The present invention relates to a fermentation process to have a high productivity with a novel mutant of Pichia sp., more specifically, for preparing erythritol under optimal fermentation conditions for maximum erythritol production by optimizing the environmental conditions of culture such as pH, temperature and by controlling osmotic pressure. A two-stage fermentation was performed to control osmotic pressure. Osmotic pressure was adjusted to a low level during growth phase and to a relatively high level during production phase by adding continuously glucose and NaCl or KCl. Therefore, erythritol production could be increased.

摘要翻译： 本发明涉及一种利用毕赤酵母的新型突变体具有高生产率的发酵方法，更具体地说，用于通过优化培养物的环境条件如pH，温度和通过以下方式在最佳发酵条件下制备赤藓糖醇来最大化赤藓糖醇生产： 控制渗透压。 进行两阶段发酵以控制渗透压。 通过连续添加葡萄糖和NaCl或KCl，在生长阶段将渗透压调节至低水平，并在生产阶段调节至较高水平。 因此，可以增加赤藓糖醇的生产。

公开(公告)号：US06316245B1

公开(公告)日：2001-11-13

申请号：US09383384

申请日：1999-08-26

申请人： Sang Yong Kim ,  Deok Kun Oh ,  Soo Ryun Jung

发明人： Sang Yong Kim ,  Deok Kun Oh ,  Soo Ryun Jung

IPC分类号： C12N116

CPC分类号： C12R1/84 ,  C12P7/18 ,  Y10S435/938

摘要： The present invention relates to a fermentation process to have a high productivity with a novel mutant of Pichia sp., more specifically, for preparing erythritol under optimal fermentation conditions for maximum erythritol production by optimizing the environmental conditions of culture such as pH, temperature and by controlling osmotic pressure. A two-stage fermentation was performed to control osmotic pressure. Osmotic pressure was adjusted to a low level during growth phase and to a relatively high level during production phase by adding continuously glucose and NaCl or KCl. Therefore, erythritol production could be increased.

摘要翻译： 本发明涉及一种利用毕赤酵母的新型突变体具有高生产率的发酵方法，更具体地说，用于通过优化培养物的环境条件如pH，温度和通过以下方式在最佳发酵条件下制备赤藓糖醇来最大化赤藓糖醇生产： 控制渗透压。 进行两阶段发酵以控制渗透压。 通过连续添加葡萄糖和NaCl或KCl，在生长阶段将渗透压调节至低水平，并在生产阶段调节至较高水平。 因此，可以增加赤藓糖醇的生产。

公开(公告)号：US06270815B1

公开(公告)日：2001-08-07

申请号：US09468091

申请日：1999-12-21

申请人： Sang Yong Kim ,  Deok Kun Oh ,  Bong Soo Noh ,  Soo Ryun Jung ,  Kyung Ah Kim

发明人： Sang Yong Kim ,  Deok Kun Oh ,  Bong Soo Noh ,  Soo Ryun Jung ,  Kyung Ah Kim

IPC分类号： C12P718

CPC分类号： C12R1/88 ,  C12P7/18

摘要： The present invention relates to a fermentation process to have a high productivity with a novel mutant of Torula sp. DS101 (KCMM-10171), more specifically, for preparing erythritol using mother liquor produced from purification process, which comprises 30˜60% of trehalulose, 10˜30% of palatinose, 5˜15% of fructose, 5˜25% of glucose and 0˜10% of sucrose.

摘要翻译： 本发明涉及用Torula sp的新型突变体具有高生产率的发酵方法。 DS101（KCMM-10171），更具体地说，用纯化工艺生产的母液制备赤藓糖醇，其中包括30〜60％的海藻糖，10〜30％的帕拉金糖，5〜15％的果糖，5〜25％的葡萄糖 和0〜10％的蔗糖。

公开(公告)号：US5998181A

公开(公告)日：1999-12-07

申请号：US42006

申请日：1998-03-13

申请人： Sang Yong Kim ,  Deok Kun Oh ,  Soo Ryun Jung

发明人： Sang Yong Kim ,  Deok Kun Oh ,  Soo Ryun Jung

IPC分类号： C12P7/18 ,  C12P19/02 ,  C12N1/20

CPC分类号： C12R1/74 ,  C12P19/02 ,  C12P7/18 ,  Y10S435/922

摘要： The present invention relates to a fermentation process for preparing xylitol with high productivity and high yield using a novel strain of Candida tropicalis, more specifically, for preparing xylitol under optimal fermentation conditions for maximum xylitol production by optimizing the composition of medium containing xylose and the environmental conditions of culture such as pH, temperature and DO concentration.

摘要翻译： 本发明涉及使用热带假丝酵母的新型菌株高生产率和高产量制备木糖醇的发酵方法，更具体地说，在最佳发酵条件下通过优化含有木糖的培养基的组成和环境的木糖醇生产来最大化木糖醇生产 培养条件如pH，温度和DO浓度。

公开(公告)号：US5989878A

公开(公告)日：1999-11-23

申请号：US21304

申请日：1998-02-10

申请人： Sang Yong Kim ,  Deok Kun Oh ,  Soo Ryun Jung

发明人： Sang Yong Kim ,  Deok Kun Oh ,  Soo Ryun Jung

IPC分类号： C12P19/02 ,  C12N1/14 ,  C12P7/18

CPC分类号： C12R1/645 ,  C12P7/18 ,  Y10S435/911

摘要： The present invention relates to a fermentation process for preparing erythritol with high productivity with novel mutant of Trigonopsis variabilis, more specifically, for preparing erythritol under optimal fermentation conditions for maximum erythritol production by optimizing the environmental conditions of culture such as pH, temperature and controlling osmotic pressure. A two-stage fermentation was performed to control osmotic pressure. Osmotic pressure was adjusted to a low level during growth phase and to a high level during production phase by adding glucose and NaCl. Therefore, erythritol production could be increased due to the increased mutant cells.

公开(公告)号：US5686277A

公开(公告)日：1997-11-11

申请号：US736622

申请日：1996-10-24

申请人： Sang Yong Kim ,  Deok Kun Oh ,  Jin Hwan Choi ,  Soo Eun Kim

发明人： Sang Yong Kim ,  Deok Kun Oh ,  Jin Hwan Choi ,  Soo Eun Kim

IPC分类号： C12N1/20 ,  C12P7/18 ,  C12P19/04 ,  C12N1/16

CPC分类号： C12R1/72 ,  C12P7/18 ,  Y10S435/921

摘要： The present invention relates to a fermentation process for preparing xylitol with high productivity using novel mutant cells of Candida parapsilosis, more specifically, for preparing xylitol under optimal fermentation conditions for maximum xylitol production by optimizing the composition of medium containing xylose and the environmental conditions of culture such as pH, temperature and DO concentration, anal controlling the concentration of mutant cells.

摘要翻译： 本发明涉及使用新型假丝酵母突变型细胞制备木糖醇的发酵方法，更具体地说，用于通过优化含有木糖的培养基的组成和培养环境条件来最佳木糖醇生产的最佳发酵条件下制备木糖醇 如pH，温度和DO浓度，肛门控制突变细胞的浓度。

公开(公告)号：US08609391B2

公开(公告)日：2013-12-17

申请号：US13503081

申请日：2010-10-22

申请人： Deok Kun Oh ,  Soo Jin Yeom

发明人： Deok Kun Oh ,  Soo Jin Yeom

IPC分类号： C12N9/90 ,  C12N9/00 ,  C07K1/00

CPC分类号： C12N9/90 ,  C12P19/02 ,  C12P19/24 ,  C12Y503/01008

摘要： New mannose-6-phasphate isomerase, mutant enzyme thereof, and a method of producing L-ribose using the enzyme are provided, and more specifically, mannose-6-phosphate isomerase, mutant enzyme thereof, recombinant expression vectors including relevant genes, microorganisms transformed with the vectors, a method of producing mannose-6-phosphate isomerase or mutant thereof in bulk using them, and a high yield method of producing L-ribose using the mannose-6-isomerase or the mutant thereof, are provided.

摘要翻译： 提供了新的甘露糖-6-磷酸异构酶，其突变体酶和使用该酶生产L-核糖的方法，更具体地说，甘露糖-6-磷酸异构酶，其突变酶，包含相关基因的重组表达载体，微生物转化 使用载体，使用它们大量生产甘露糖-6-磷酸异构酶或其突变体的方法，以及使用甘露糖-6-异构酶或其突变体产生L-核糖的高产率方法。

公开(公告)号：US09217166B2

公开(公告)日：2015-12-22

申请号：US14240647

申请日：2012-08-21

申请人： Yang Hee Kim ,  Jin Ha Kim ,  Young Mi Lee ,  Young Ho Hong ,  Min Hae Kim ,  Seong Bo Kim ,  Seung Won Park ,  Seung Hyun Oh ,  Deok Kun Oh ,  Jin Geun Choi

发明人： Yang Hee Kim ,  Jin Ha Kim ,  Young Mi Lee ,  Young Ho Hong ,  Min Hae Kim ,  Seong Bo Kim ,  Seung Won Park ,  Seung Hyun Oh ,  Deok Kun Oh ,  Jin Geun Choi

IPC分类号： C12P19/02 ,  C12P19/24 ,  C12N9/90

CPC分类号： C12P19/24 ,  C12N9/90 ,  C12P19/02 ,  C12Y501/03

摘要： The present invention relates to a D-psicose 3-epimerase variant with improved thermostability by substituting an amino acid at a specific position of an amino acid sequence of a wild type D-psicose 3-epimerase. Further, the present invention provides a recombinant expression vector including a gene of the D-psicose 3-epimerase variant, and a recombinant strain transformed with the recombinant expression vector. Furthermore, the present invention provides an immobilized reactor prepared using the D-psicose 3-epimerase variant or the recombinant strain, and a method of continuously producing D-psicose using the immobilized reactor.

摘要翻译： 本发明涉及通过用野生型D-灵糖3-差向异构酶的氨基酸序列的特定位置的氨基酸取代而具有改进的热稳定性的D-灵敏的3-差向异构酶变体。 此外，本发明提供了包含D-灵长类3-差向异构酶变体的基因和用重组表达载体转化的重组菌株的重组表达载体。 此外，本发明提供了使用D-灵长类3-差向异构酶变体或重组菌株制备的固定化反应器，以及使用固定化反应器连续生产D-灵敏蛋白的方法。

公开(公告)号：US20120270274A1

公开(公告)日：2012-10-25

申请号：US13503081

申请日：2010-10-22

申请人： Deok Kun Oh ,  Soo Jin Yeom

发明人： Deok Kun Oh ,  Soo Jin Yeom

IPC分类号： C12N9/90 ,  C12N15/61 ,  C12P19/24

CPC分类号： C12N9/90 ,  C12P19/02 ,  C12P19/24 ,  C12Y503/01008

摘要： New mannose-6-phasphate isomerase, mutant enzyme thereof, and a method of producing L-ribose using the enzyme are provided, and more specifically, mannose-6-phosphate isomerase, mutant enzyme thereof, recombinant expression vectors including relevant genes, microorganisms transformed with the vectors, a method of producing mannose-6-phosphate isomerase or mutant thereof in bulk using them, and a high yield method of producing L-ribose using the mannose-6-isomerase or the mutant thereof, are provided.

摘要翻译： 提供了新的甘露糖-6-磷酸异构酶，其突变体酶和使用该酶生产L-核糖的方法，更具体地说，甘露糖-6-磷酸异构酶，其突变酶，包含相关基因的重组表达载体，微生物转化 使用载体，使用它们大量生产甘露糖-6-磷酸异构酶或其突变体的方法，以及使用甘露糖-6-异构酶或其突变体产生L-核糖的高产率方法。