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1.发明申请公开(公告)号：US20100189717A1
公开(公告)日：2010-07-29
申请号：US12668766
申请日：2008-07-09
申请人： Sang-Lin Kim , Hyun-Kwang Tan , Sang-Min Lim , Wuk-Sang Ryu , Hahn-Sun Jung , Song-Jae Lee , Cheon-Ik Park , Seung-Hoon Kang , Dong Il Kim
发明人： Sang-Lin Kim , Hyun-Kwang Tan , Sang-Min Lim , Wuk-Sang Ryu , Hahn-Sun Jung , Song-Jae Lee , Cheon-Ik Park , Seung-Hoon Kang , Dong Il Kim
CPC分类号： C07K14/70521 , C07K2319/30 , C12N9/1051 , C12N15/8257 , C12P21/005
摘要： The present invention provides a recombinant vector pBI-3D-hGalT or pBI-35S-hGalT containing a human β1,4-galactosyltransferase gene; a cell line transformed with a recombinant vector pMYN414 containing a cytotoxic T-lymphocyte anti-gen A-immunoglobulin (CTLA4Ig) fusion protein gene and the recombinant vector pBI-3D-hGalT or pBI-355-hGalT; and a method for producing a plant-derived recombinant human CTLA4Ig (CTLA4Igp) fusion protein with a human glycan structure using the same. The plant cell-derived recombinant human CTLA4Ig fusion protein (CTLA4Igp), which has a human glycan structure and is produced according to the present invention, exhibits an improved in vivo half life as compared to conventional plant-derived proteins, due to the presence of a human-like glycan structure. Consequently, the present invention using the plant expression system enables low-cost mass production of a CTLA4Igp fusion protein having an immunosuppressive activity comparable to that of the CTLA4IgM fusion protein expressed in conventional animal cell expression systems.
摘要翻译： 本发明提供了含有人和重组1,4-半乳糖基转移酶基因的重组载体pBI-3D-hGalT或pBI-35S-hGalT; 用含有细胞毒性T淋巴细胞抗A型免疫球蛋白（CTLA4Ig）融合蛋白基因和重组载体pBI-3D-hGalT或pBI-355-hGalT的重组载体pMYN414转化的细胞系; 以及使用其使用具有人聚糖结构的植物来源的重组人CTLA4Ig（CTLA4Igp）融合蛋白的方法。 具有人聚糖结构并根据本发明生产的植物细胞衍生的重组人CTLA4Ig融合蛋白（CTLA4Igp）与常规植物衍生的蛋白质相比显示出与常规植物衍生的蛋白质相比改善的体内半衰期， 人类聚糖结构。 因此，使用植物表达系统的本发明能够低成本大规模生产具有与常规动物细胞表达系统中表达的CTLA4IgM融合蛋白相当的免疫抑制活性的CTLA4Igp融合蛋白。
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2.发明申请METHOD FOR PRODUCING TARGET PROTEINS USING AMINO ACIDS AND PYRUVIC ACIDS IN CULTURE OF PLANT CELLS 失效在植物细胞培养中使用氨基酸和吡啶酸生产目标蛋白的方法公开(公告)号：US20090181428A1
公开(公告)日：2009-07-16
申请号：US12160049
申请日：2007-09-19
申请人： Sang-Lin Kim , Hyun-Kwang Tan , Sang-Min Lim , Wuk-Sang Ryu , Hahn-Sun Jung , Song-Jae Lee , Cheon-Ik Park , Seung-Hoon Kang , Dong-Il Kim
发明人： Sang-Lin Kim , Hyun-Kwang Tan , Sang-Min Lim , Wuk-Sang Ryu , Hahn-Sun Jung , Song-Jae Lee , Cheon-Ik Park , Seung-Hoon Kang , Dong-Il Kim
IPC分类号： C12P21/00
CPC分类号： C12P21/00 , A01H4/00 , C12N15/8237
摘要： Provided is a method for producing a target protein via cultivation of transgenic plant cells comprising a promoter capable of expressing the protein under sugar-free conditions or in response to the depletion of sugar and a gene encoding the target protein, without exchange of a cell growth medium with a sugar-depleted medium. The method comprises 1) culturing transgenic plant cells in a sugar-rich medium to grow plant cells; and 2) culturing the transgenic plant cells with addition of an amino acid mixture to the culture of Step 1 without exchange of a cell growth medium with a sugar-depleted medium, thereby expressing a target protein. The method of the present invention enables commercial-scale production of recombinant proteins via establishment of optimized culture conditions by addition of an amino acid mixture to induce protein expression without exchange of a cell growth medium with a sugar-free medium, and addition of pyruvic acid during the induction period of protein expression to thereby enhance the production yield of target proteins.
摘要翻译： 提供了通过培养转基因植物细胞来生产靶蛋白的方法，所述转基因植物细胞包含能够在无糖条件下表达该蛋白质的启动子，或者响应于糖的消耗和编码靶蛋白质的基因而不交换细胞生长 培养基含糖耗竭的培养基。 该方法包括：1）在富含糖的培养基中培养转基因植物细胞以生长植物细胞; 和2）向步骤1的培养物中加入氨基酸混合物的转基因植物细胞培养，而不用无糖培养基交换细胞生长培养基，从而表达靶蛋白。 本发明的方法可以通过添加氨基酸混合物建立优化的培养条件来产生重组蛋白质，从而诱导蛋白质表达而不用无糖培养基交换细胞生长培养基，并加入丙酮酸 在蛋白质表达的诱导期，从而提高靶蛋白的产量。
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3.发明授权Method for producing target proteins using amino acids and pyruvic acids in culture of plant cells 失效在植物细胞培养中使用氨基酸和丙酮酸生产靶蛋白的方法公开(公告)号：US08003850B2
公开(公告)日：2011-08-23
申请号：US12160049
申请日：2007-09-19
申请人： Sang-Lin Kim , Hyun-Kwang Tan , Sang-Min Lim , Wuk-Sang Ryu , Hahn-Sun Jung , Song-Jae Lee , Cheon-Ik Park , Seung-Hoon Kang , Dong-Il Kim
发明人： Sang-Lin Kim , Hyun-Kwang Tan , Sang-Min Lim , Wuk-Sang Ryu , Hahn-Sun Jung , Song-Jae Lee , Cheon-Ik Park , Seung-Hoon Kang , Dong-Il Kim
CPC分类号： C12P21/00 , A01H4/00 , C12N15/8237
摘要： Provided is a method for producing a target protein via cultivation of transgenic plant cells comprising a promoter capable of expressing the protein under sugar-free conditions in the response to the depletion of sugar and a gene encoding the target protein, without exchange of a cell growth medium with a sugar-depleted medium comprising the addition of an amino acid mixture to the sugar-rich medium used to grow the plant cells.
摘要翻译： 提供了通过培养转基因植物细胞来生产靶蛋白的方法，所述转基因植物细胞包含能够在无糖条件下表达蛋白质的启动子，以响应于糖的消耗和编码靶蛋白的基因，而不交换细胞生长 培养基中加入含糖的培养基，包括向用于生长植物细胞的富糖培养基中加入氨基酸混合物。
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公开(公告)号：US08395603B2
公开(公告)日：2013-03-12
申请号：US12565217
申请日：2009-09-23
申请人： Jae-Hoon Myung , Young-Joo Park , Joon-Hyun Kim , Sang-Min Lim
发明人： Jae-Hoon Myung , Young-Joo Park , Joon-Hyun Kim , Sang-Min Lim
IPC分类号： G06F3/038
CPC分类号： G09G3/3696 , G09G3/006 , G09G2330/04
摘要： An electronic device including a display device, and a driving method thereof, in which the display device includes a central processing unit, a display device, and a feedback unit. The central processing unit provides image signals and input control signals, and the display device displays an image based on the image signals and the input control signals. The feedback unit is connected between the central processing unit and the display device and transmits a signal, including information on whether static electricity is applied to the display device, to the central processing unit. The central processing unit initializes a driving condition when the static electricity is applied to the display device and a display operation error occurs.
摘要翻译： 一种包括显示装置及其驱动方法的电子装置，其中显示装置包括中央处理单元，显示装置和反馈单元。 中央处理单元提供图像信号和输入控制信号，并且显示装置基于图像信号和输入控制信号显示图像。 反馈单元连接在中央处理单元和显示设备之间，并且向中央处理单元发送包括关于静电是否施加到显示设备的信息的信号。 中央处理单元在将静电施加到显示装置时初始化驱动条件并发生显示操作错误。
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公开(公告)号：US20120242641A1
公开(公告)日：2012-09-27
申请号：US13200331
申请日：2011-09-23
申请人： Kwangsae Lee , Jeonggeun Yoo , Sang-Min Lim
发明人： Kwangsae Lee , Jeonggeun Yoo , Sang-Min Lim
IPC分类号： G09G5/00
CPC分类号： G09G3/3655
摘要： Embodiments may disclose a display device which includes a display panel including a display pixel displaying an image in response to a common voltage and a data voltage and a sensing pixel outputting a feedback voltage in response to the common voltage and a reference voltage; and a driving circuit unit supplying the data voltage and the reference voltage to the display pixel and the sensing pixel, respectively. The driving circuit unit includes a common voltage estimating part comparing the reference voltage and the feedback voltage to generate a counter signal having a counter value that is stepwise varied according to the comparing of the reference voltage and the feedback voltage; and a common voltage adjusting part stepwise varying the common voltage supplied to the display panel in response to the counter value.
摘要翻译： 实施例可以公开一种显示装置，其包括响应于公共电压和数据电压而显示图像的显示像素和响应于公共电压和参考电压而输出反馈电压的感测像素的显示面板; 以及分别向显示像素和感测像素提供数据电压和参考电压的驱动电路单元。 驱动电路单元包括公共电压估计部分，用于比较参考电压和反馈电压，以产生具有根据参考电压和反馈电压的比较而逐步变化的计数器值的计数器信号; 以及公共电压调整部件，其响应于所述计数器值逐步改变提供给所述显示面板的公共电压。
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公开(公告)号：US08988410B2
公开(公告)日：2015-03-24
申请号：US13200331
申请日：2011-09-23
申请人： Kwangsae Lee , Jeonggeun Yoo , Sang-Min Lim
发明人： Kwangsae Lee , Jeonggeun Yoo , Sang-Min Lim
CPC分类号： G09G3/3655
摘要： A display panel includes a display pixel displaying an image in response to a common voltage and a data voltage and a sensing pixel outputting a feedback voltage in response to the common voltage and a reference voltage; and a driving circuit unit supplying the data voltage and the reference voltage to the display pixel and the sensing pixel, respectively. The driving circuit unit includes a common voltage estimating part comparing the reference voltage and the feedback voltage to generate a counter signal having a counter value that is stepwise varied according to the comparing of the reference voltage and the feedback voltage; and a common voltage adjusting part stepwise varying the common voltage supplied to the display panel in response to the counter value.
摘要翻译： 显示面板包括响应于公共电压和数据电压显示图像的显示像素和响应于公共电压和参考电压而输出反馈电压的感测像素; 以及分别向显示像素和感测像素提供数据电压和参考电压的驱动电路单元。 驱动电路单元包括公共电压估计部分，用于比较参考电压和反馈电压，以产生具有根据参考电压和反馈电压的比较而逐步变化的计数器值的计数器信号; 以及公共电压调节部件，其响应于所述计数器值逐步改变提供给所述显示面板的公共电压。
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公开(公告)号：US20080180431A1
公开(公告)日：2008-07-31
申请号：US11972806
申请日：2008-01-11
申请人： Jae-Hoon Myung , Young-Joo Park , Joon-Hyun Kim , Sang-Min Lim
发明人： Jae-Hoon Myung , Young-Joo Park , Joon-Hyun Kim , Sang-Min Lim
IPC分类号： G09G5/00
CPC分类号： G09G3/3696 , G09G3/006 , G09G2330/04
摘要： An electronic device including a display device, and a driving method thereof. The electronic device including the display device includes a central processing unit, a display device, and a feedback unit. The central processing unit provides image signals and input control signals. The display device displays an image based on the image signals and the input control signals. The feedback unit is connected between the central processing unit and the display device and transmits a signal including information on whether static electricity is applied to the display device to the central processing unit. The central processing unit initializes a driving condition when the static electricity is applied to the display device and a display operation error occurs.
摘要翻译： 一种包括显示装置的电子装置及其驱动方法。 包括显示装置的电子设备包括中央处理单元，显示设备和反馈单元。 中央处理单元提供图像信号和输入控制信号。 显示装置根据图像信号和输入控制信号显示图像。 反馈单元连接在中央处理单元和显示装置之间，并且将包括关于静电是否施加到显示装置的信息的信号发送到中央处理单元。 中央处理单元在将静电施加到显示装置时初始化驱动条件并发生显示操作错误。
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8.发明授权Gamma voltage controller, gradation voltage generator, and display device including them 有权伽玛电压控制器，灰度电压发生器和包括它们的显示装置公开(公告)号：US08836733B2
公开(公告)日：2014-09-16
申请号：US13087162
申请日：2011-04-14
申请人： Seung-Wook Lee , Sang-Min Lim
发明人： Seung-Wook Lee , Sang-Min Lim
IPC分类号： G09G5/10
CPC分类号： G09G5/10 , G09G3/3696 , G09G5/02 , G09G2320/0271 , G09G2320/0276 , G09G2320/0673
摘要： The present invention relates to a gamma voltage controller, a gradation voltage generator, and a display device including them. The gamma voltage controller corrects luminance in high and low gradations according to a color mode of a display panel, and generates a plurality of gradation voltages through inflection point adjustment, thus minimizing gradation mismatch between color modes.
摘要翻译： 本发明涉及一种伽马电压控制器，灰度电压发生器和包括它们的显示装置。 伽马电压控制器根据显示面板的颜色模式校正高和低灰度的亮度，并且通过拐点调整产生多个灰度电压，从而最小化色彩模式之间的灰度失配。
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9.发明申请PHOTOBIOREACTOR FOR MASS CULTURE OF MICROALGAE, AND METHOD FOR CULTURING MICROALGAE BY USING SAME 审中-公开微藻培养基的光电感应器及其使用方法培养微藻公开(公告)号：US20130052719A1
公开(公告)日：2013-02-28
申请号：US13580635
申请日：2011-02-22
申请人： Choul-Gyun Lee , Sang-Min Lim , Jae-Han Bae , Kwang-kuk Cho , Z-Hun Kim , Sin-Ae Kang , Hye-Jung Kim
发明人： Choul-Gyun Lee , Sang-Min Lim , Jae-Han Bae , Kwang-kuk Cho , Z-Hun Kim , Sin-Ae Kang , Hye-Jung Kim
摘要： The present invention relates to a photobioreactor for mass culture of microalgae. The present invention provides a photobioreactor for mass culture of microalgae, comprising: a culture container comprising external walls which comprise an optical filter zone capable of selective penetration or blocking of a part of wavelength or region from sunlight and/or a heat conversion zone for selectively absorbing a part of wavelength or region from sunlight to convert the same into heat, and a reaction chamber which is three-dimensionally formed so as to accommodate microalgae and is an inner space restricted by the external walls; and a coupling means for connecting a floating means which is connected to the culture container or the culture container to the bottom of the water or a structure on the water, thereby locating the culture container near the surface of the water for the exposure of the culture container to sunlight.
摘要翻译： 本发明涉及微藻大规模培养的光生物反应器。 本发明提供了一种用于微藻大规模培养的光生物反应器，包括：培养容器，其包括外壁，其包括能够选择性地穿透或阻挡一部分波长或区域的阳光和/或热转化区用于选择性地 从阳光中吸收一部分波长或区域以将其转化为热;以及反应室，其三维地形成以容纳微藻，并且是被外壁限制的内部空间; 以及用于将连接到培养容器或培养容器的浮动装置连接到水的底部或水上的结构的联接装置，从而将培养容器定位在靠近水面的表面以暴露培养物 容器到阳光。
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10.发明申请Gamma Voltage Controller, Gradation Voltage Generator, and Display Device Including Them 有权伽玛电压控制器，梯度电压发生器和包括它们的显示设备公开(公告)号：US20120162272A1
公开(公告)日：2012-06-28
申请号：US13087162
申请日：2011-04-14
申请人： Seung-Wook Lee , Sang-Min Lim
发明人： Seung-Wook Lee , Sang-Min Lim
IPC分类号： G09G5/10
CPC分类号： G09G5/10 , G09G3/3696 , G09G5/02 , G09G2320/0271 , G09G2320/0276 , G09G2320/0673
摘要： The present invention relates to a gamma voltage controller, a gradation voltage generator, and a display device including them. The gamma voltage controller corrects luminance in high and low gradations according to a color mode of a display panel, and generates a plurality of gradation voltages through inflection point adjustment, thus minimizing gradation mismatch between color modes.
摘要翻译： 本发明涉及一种伽马电压控制器，灰度电压发生器和包括它们的显示装置。 伽马电压控制器根据显示面板的颜色模式校正高和低灰度的亮度，并且通过拐点调整产生多个灰度电压，从而最小化色彩模式之间的灰度失配。
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