公开(公告)号：US06500944B2

公开(公告)日：2002-12-31

申请号：US08895136

申请日：1997-07-16

Applicant: Saroj K. Roy ,  Jin-Yan Tang

Inventor： Saroj K. Roy ,  Jin-Yan Tang

IPC: C07H2100

CPC classification number: C07D285/01 ,  C07H21/00

Abstract: The invention relates to the chemical synthesis of oligonucleotides and to chemical entities useful in such synthesis. More particularly, the invention relates to sulfurization of the internucleotide linkages of oligonucleotides. The invention provides a process to synthesize new sulfur transfer reagents and a process for their use in sulfurizing oligonucleotides. The sulfur transfer reagents according to the invention are inexpensive to make, stable in storage and in solution, and highly efficient in sulfurization.

Abstract translation: 本发明涉及寡核苷酸的化学合成以及可用于这种合成的化学实体。 更具体地，本发明涉及寡核苷酸的核苷酸间键的硫化。 本发明提供合成新的硫转移试剂的方法及其在硫化寡核苷酸中的用途。 根据本发明的硫转移试剂制备成本低，储存稳定，溶解稳定，硫化效率高。

公开(公告)号：US5214135A

公开(公告)日：1993-05-25

申请号：US753077

申请日：1991-08-30

Applicant: Suresh C. Srivastava ,  Saroj K. Roy

Inventor： Suresh C. Srivastava ,  Saroj K. Roy

IPC: C07H19/06 ,  C07H19/10 ,  C07H19/16 ,  C07H19/20 ,  C07H21/00

CPC classification number: C07H19/06 ,  C07H19/10 ,  C07H19/16 ,  C07H19/20 ,  C07H21/00

Abstract: This invention is directed to N (amino)-protected-2'-O-methyl (methoxy)-5'-dimethoxytrityl (dimethoxytrityl) ribonucleosides (Group I compounds) and to N-protected-2'-O-methyl-5'-dimethoxytrityl-3'-ribonucleoside cyanoethyl N,N-diisopropyl phosphoramidites (Group II compounds). This invention is further directed to the processes involved in producing these compounds. The purification processes produce products in Group I with purity in excess of 99.50% and up to 99.8%. The purity of the products in Group II ranges in excess of 99.8% and up to 99.9%.

Abstract translation: 本发明涉及N（氨基）保护的2'-O-甲基（甲氧基）-5'-二甲氧基三苯甲基（二甲氧基三苯甲基）核糖核苷（I类化合物）和N-保护的2'-O-甲基-5' 二甲氧基三苯甲基-3'-核糖核苷氰基乙基N，N-二异丙基亚磷酰胺（II类化合物）。 本发明进一步涉及生产这些化合物所涉及的方法。 纯化过程产生纯度超过99.50％且高达99.8％的I组产品。 II类产品的纯度范围超过99.8％，高达99.9％。