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公开(公告)号：US09464304B2
公开(公告)日：2016-10-11
申请号：US12628144
申请日：2009-11-30
CPC分类号： C12P7/6427 , C07C1/2078 , C07K2319/01 , C10L1/02 , C12N9/00 , C12N9/16 , C12N9/2402 , C12N15/79 , C12P7/20 , C12P7/64 , C12P7/6409 , C12P7/6418 , C12P7/6463 , C12P7/649 , C12Y301/02014 , C12Y302/01026 , Y02E50/13 , Y02P20/52 , Y02P60/877 , Y02T50/678
摘要： Methods are disclosed for cultivation of oleaginous microorganisms on feedstocks including depolymerized cellulosic material such as corn stover, Miscanthus, forage sorghum, sugar beet pulp and sugar cane bagasse. Also disclosed are methods of manufacturing novel triglyceride oil compositions using cellulosic feedstocks, including oil compositions comprising fatty acids such as those having chain lengths of 8, 10, 12, and 14 carbon atoms.
摘要翻译： 公开了用于在原料上培养含油微生物的方法，包括解聚的纤维素材料如玉米秸秆，芒草，饲料高粱，甜菜浆和甘蔗渣。 还公开了使用纤维素原料制造新型甘油三酯油组合物的方法，包括含有脂肪酸的油组合物，例如链长为8,10,12和14个碳原子的那些。
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公开(公告)号：US20130089916A1
公开(公告)日：2013-04-11
申请号：US13628039
申请日：2012-09-26
IPC分类号： C12N15/79
CPC分类号： C12P7/6427 , C07C1/2078 , C07K2319/01 , C10L1/02 , C12N9/00 , C12N9/16 , C12N9/2402 , C12N15/79 , C12P7/20 , C12P7/64 , C12P7/6409 , C12P7/6418 , C12P7/6463 , C12P7/649 , C12Y301/02014 , C12Y302/01026 , Y02E50/13 , Y02P20/52 , Y02P60/877 , Y02T50/678
摘要： Novel gene sequences from microalgae are disclosed, as well as novel gene sequences useful in the manufacture of triglyceride oils. Also disclosed are sequences and vectors that allow microalgae to be cultivated on sugar cane and sugar beets as a feedstock. In some embodiments, the vectors are useful for the purpose of performing targeted modifications to the nuclear genome of heterotrophic microalgae.
摘要翻译： 公开了来自微藻的新型基因序列，以及可用于制备甘油三酯油的新型基因序列。 还公开了允许将微藻种植在甘蔗和甜菜作为原料的序列和载体。 在一些实施方案中，载体可用于进行对异养微藻的核基因组的靶向修饰。
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公开(公告)号：US08222010B2
公开(公告)日：2012-07-17
申请号：US12981409
申请日：2010-12-29
CPC分类号： C12P7/6427 , C07C1/2078 , C07K2319/01 , C10L1/02 , C12N9/00 , C12N9/16 , C12N9/2402 , C12N15/79 , C12P7/20 , C12P7/64 , C12P7/6409 , C12P7/6418 , C12P7/6463 , C12P7/649 , C12Y301/02014 , C12Y302/01026 , Y02E50/13 , Y02P20/52 , Y02P60/877 , Y02T50/678
摘要： Disclosed herein are methods of manufacturing renewable chemicals through the manufacture of novel triglyceride oils followed by chemical modification of the oils. Methods such as transesterification, hydrogenation, hydrocracking, deoxygenation, isomerization, interesterification, hydroxylation, hydrolysis and saponification are disclosed. Novel oils containing fatty acid chain lengths of C8, C10, C12 or C14 are also disclosed and are useful as feedstocks in the methods of the invention.
摘要翻译： 本文公开了通过制备新型甘油三酯油然后化学改性油来制造可再生化学品的方法。 公开了酯交换，氢化，加氢裂化，脱氧，异构化，酯交换，羟基化，水解和皂化等方法。 还公开了含有C8，C10，C12或C14脂肪酸链长度的新型油，并且可用作本发明方法中的原料。
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公开(公告)号：US20110165634A1
公开(公告)日：2011-07-07
申请号：US12981409
申请日：2010-12-29
CPC分类号： C12P7/6427 , C07C1/2078 , C07K2319/01 , C10L1/02 , C12N9/00 , C12N9/16 , C12N9/2402 , C12N15/79 , C12P7/20 , C12P7/64 , C12P7/6409 , C12P7/6418 , C12P7/6463 , C12P7/649 , C12Y301/02014 , C12Y302/01026 , Y02E50/13 , Y02P20/52 , Y02P60/877 , Y02T50/678
摘要： Disclosed herein are methods of manufacturing renewable chemicals through the manufacture of novel triglyceride oils followed by chemical modification of the oils. Methods such as transesterification, hydrogenation, hydrocracking, deoxygenation, isomerization, interesterification, hydroxylation, hydrolysis and saponification are disclosed. Novel oils containing fatty acid chain lengths of C8, C10, C12 or C14 are also disclosed and are useful as feedstocks in the methods of the invention.
摘要翻译： 本文公开了通过制备新型甘油三酯油然后化学改性油来制造可再生化学品的方法。 公开了酯交换，氢化，加氢裂化，脱氧，异构化，酯交换，羟基化，水解和皂化等方法。 还公开了含有C8，C10，C12或C14脂肪酸链长度的新型油，并且可用作本发明方法中的原料。
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公开(公告)号：US09062294B2
公开(公告)日：2015-06-23
申请号：US13555009
申请日：2012-07-20
IPC分类号： C12N9/20 , C12N9/24 , C12P7/64 , C10L1/02 , C12N9/00 , C12N9/16 , C12P7/20 , C12N15/79 , C07C1/207
CPC分类号： C12P7/6427 , C07C1/2078 , C07K2319/01 , C10L1/02 , C12N9/00 , C12N9/16 , C12N9/2402 , C12N15/79 , C12P7/20 , C12P7/64 , C12P7/6409 , C12P7/6418 , C12P7/6463 , C12P7/649 , C12Y301/02014 , C12Y302/01026 , Y02E50/13 , Y02P20/52 , Y02P60/877 , Y02T50/678
摘要： Disclosed herein are methods of manufacturing renewable chemicals through the manufacture of novel triglyceride oils followed by chemical modification of the oils. Methods such as transesterification, hydrogenation, hydrocracking, deoxygenation, isomerization, interesterification, hydroxylation, hydrolysis and saponification are disclosed. Novel oils containing fatty acid chain lengths of C8, C10, C12 or C14 are also disclosed and are useful as feedstocks in the methods of the invention.
摘要翻译： 本文公开了通过制备新型甘油三酯油然后化学改性油来制造可再生化学品的方法。 公开了酯交换，氢化，加氢裂化，脱氧，异构化，酯交换，羟基化，水解和皂化等方法。 还公开了含有C8，C10，C12或C14脂肪酸链长度的新型油，并且可用作本发明方法中的原料。
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公开(公告)号：US08772575B2
公开(公告)日：2014-07-08
申请号：US13628039
申请日：2012-09-26
CPC分类号： C12P7/6427 , C07C1/2078 , C07K2319/01 , C10L1/02 , C12N9/00 , C12N9/16 , C12N9/2402 , C12N15/79 , C12P7/20 , C12P7/64 , C12P7/6409 , C12P7/6418 , C12P7/6463 , C12P7/649 , C12Y301/02014 , C12Y302/01026 , Y02E50/13 , Y02P20/52 , Y02P60/877 , Y02T50/678
摘要： Novel gene sequences from microalgae are disclosed, as well as novel gene sequences useful in the manufacture of triglyceride oils. Also disclosed are sequences and vectors that allow microalgae to be cultivated on sugar cane and sugar beets as a feedstock. In some embodiments, the vectors are useful for the purpose of performing targeted modifications to the nuclear genome of heterotrophic microalgae.
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公开(公告)号：US08697427B2
公开(公告)日：2014-04-15
申请号：US13479200
申请日：2012-05-23
CPC分类号： C12P7/6427 , C07C1/2078 , C07K2319/01 , C10L1/02 , C12N9/00 , C12N9/16 , C12N9/2402 , C12N15/79 , C12P7/20 , C12P7/64 , C12P7/6409 , C12P7/6418 , C12P7/6463 , C12P7/649 , C12Y301/02014 , C12Y302/01026 , Y02E50/13 , Y02P20/52 , Y02P60/877 , Y02T50/678
摘要： Disclosed herein are obligate heterotrophic microalgae cells containing an exogenous gene. In some embodiments the gene is a sucrose utilization gene, and further disclosed are methods of manufacturing triglyceride oils using sugar cane or sugar beets as a feedstock in a heterotrophic fermentation. In other embodiments the feedstock is depolymerized cellulosic material. Also disclosed are cells that produce medium chain fatty acids at levels not produced in non-recombinant cells of the same species and genus.
摘要翻译： 本文公开了含有外源基因的专性异养微藻细胞。 在一些实施方案中，该基因是蔗糖利用基因，并且进一步公开了使用甘蔗或甜菜作为异养发酵中的原料来制备甘油三酯油的方法。 在其它实施方案中，原料是解聚的纤维素材料。 还公开了以相同物种和属的非重组细胞中不产生的水平产生中链脂肪酸的细胞。
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公开(公告)号：US08674180B2
公开(公告)日：2014-03-18
申请号：US13621722
申请日：2012-09-17
CPC分类号： C12P7/6427 , C07C1/2078 , C07K2319/01 , C10L1/02 , C12N9/00 , C12N9/16 , C12N9/2402 , C12N15/79 , C12P7/20 , C12P7/64 , C12P7/6409 , C12P7/6418 , C12P7/6463 , C12P7/649 , C12Y301/02014 , C12Y302/01026 , Y02E50/13 , Y02P20/52 , Y02P60/877 , Y02T50/678
摘要： Novel gene sequences from microalgae are disclosed, as well as novel gene sequences useful in the manufacture of triglyceride oils. Also disclosed are sequences and vectors that allow microalgae to be cultivated on sugar cane and sugar beets as a feedstock. In some embodiments, the vectors are useful for the purpose of performing targeted modifications to the nuclear genome of heterotrophic microalgae.
摘要翻译： 公开了来自微藻的新型基因序列，以及可用于制备甘油三酯油的新型基因序列。 还公开了允许将微藻种植在甘蔗和甜菜作为原料的序列和载体。 在一些实施方案中，载体可用于进行对异养微藻的核基因组的靶向修饰。
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公开(公告)号：US08268610B2
公开(公告)日：2012-09-18
申请号：US12628147
申请日：2009-11-30
IPC分类号： A01H13/00 , C07H21/04 , C07K14/405 , C12N15/00 , C12P7/64
CPC分类号： C12P7/6427 , C07C1/2078 , C07K2319/01 , C10L1/02 , C12N9/00 , C12N9/16 , C12N9/2402 , C12N15/79 , C12P7/20 , C12P7/64 , C12P7/6409 , C12P7/6418 , C12P7/6463 , C12P7/649 , C12Y301/02014 , C12Y302/01026 , Y02E50/13 , Y02P20/52 , Y02P60/877 , Y02T50/678
摘要： Novel gene sequences from microalgae are disclosed, as well as novel gene sequences useful in the manufacture of triglyceride oils. Also disclosed are sequences and vectors that allow microalgae to be cultivated on sugar cane and sugar beets as a feedstock. In some embodiments, the vectors are useful for the purpose of performing targeted modifications to the nuclear genome of heterotrophic microalgae.
摘要翻译： 公开了来自微藻的新型基因序列，以及可用于制备甘油三酯油的新型基因序列。 还公开了允许将微藻种植在甘蔗和甜菜作为原料的序列和载体。 在一些实施方案中，载体可用于进行对异养微藻的核基因组的靶向修饰。
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公开(公告)号：US20130078709A1
公开(公告)日：2013-03-28
申请号：US13621722
申请日：2012-09-17
IPC分类号： C12N15/79
CPC分类号： C12P7/6427 , C07C1/2078 , C07K2319/01 , C10L1/02 , C12N9/00 , C12N9/16 , C12N9/2402 , C12N15/79 , C12P7/20 , C12P7/64 , C12P7/6409 , C12P7/6418 , C12P7/6463 , C12P7/649 , C12Y301/02014 , C12Y302/01026 , Y02E50/13 , Y02P20/52 , Y02P60/877 , Y02T50/678
摘要： Novel gene sequences from microalgae are disclosed, as well as novel gene sequences useful in the manufacture of triglyceride oils. Also disclosed are sequences and vectors that allow microalgae to be cultivated on sugar cane and sugar beets as a feedstock. In some embodiments, the vectors are useful for the purpose of performing targeted modifications to the nuclear genome of heterotrophic microalgae.
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