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    METHOD FOR LARGE SCALE PREPARATION OF THE ACTIVE DOMAIN OF HUMAN PROTEIN TYROSINE PHOSPHATASE WITHOUT FUSION PROTEIN
    1.
    发明申请
    METHOD FOR LARGE SCALE PREPARATION OF THE ACTIVE DOMAIN OF HUMAN PROTEIN TYROSINE PHOSPHATASE WITHOUT FUSION PROTEIN 审中-公开
    不含融合蛋白的人蛋白酪氨酸磷酸酶活性域的大规模制备方法

    公开(公告)号：US20100261213A1

    公开(公告)日：2010-10-14

    申请号：US12746438

    申请日：2008-08-04

    申请人： Seong Eon Ryu ,  Dae Gwin Jeong ,  Jae Hoon Kim ,  Seung Jun Kim ,  Sang Jeon Chung ,  Jeong Hee Son

    发明人： Seong Eon Ryu ,  Dae Gwin Jeong ,  Jae Hoon Kim ,  Seung Jun Kim ,  Sang Jeon Chung ,  Jeong Hee Son

    IPC分类号： C12Q1/42 ,  C12N9/16

    CPC分类号： C12Q1/42 ,  C12N9/16

    摘要： The present invention relates to protein tyrosine phosphatase (PTP) and a method for preparing the same, precisely, a method for expressing PTP active domain with high activity and stability without help of a fusion protein, by using computer based protein structure prediction technique. PTP prepared by the method of the present invention can be effectively used as a protein for high efficiency drug screening for the development of a novel drug, as an antigen protein for the construction of a selective antibody and as a protein for the studies of PTP structure and functions.

    摘要翻译： 本发明涉及蛋白酪氨酸磷酸酶（PTP）及其制备方法，准确地说，通过使用基于计算机的蛋白质结构预测技术，在没有融合蛋白的帮助下，表达具有高活性和稳定性的PTP活性结构域的方法。 通过本发明方法制备的PTP可以有效地用作用于高效药物筛选的蛋白质，用于开发新型药物，作为构建选择性抗体的抗原蛋白和用于研究PTP结构的蛋白质 和功能。

    Crystal of a phosphatase of regenerating liver 1 (PRL-1) polypeptide and method of crystallization thereof
    2.
    发明授权
    Crystal of a phosphatase of regenerating liver 1 (PRL-1) polypeptide and method of crystallization thereof 有权
    再生肝脏1（PRL-1）多肽的磷酸酶的晶体及其结晶方法

    公开(公告)号：US07998719B2

    公开(公告)日：2011-08-16

    申请号：US11587404

    申请日：2005-04-26

    申请人： Seong Eon Ryu ,  Dae Gwin Jeong ,  Seung-Jun Kim ,  Jae Hoon Kim ,  Jeong Hee Son

    发明人： Seong Eon Ryu ,  Dae Gwin Jeong ,  Seung-Jun Kim ,  Jae Hoon Kim ,  Jeong Hee Son

    IPC分类号： C12N9/16

    CPC分类号： C12N9/16 ,  C07K2299/00

    摘要： The present invention relates to a crystal structure of PRL-1 (Phospatase of Regenerating Liver) protein and a method of crystallization thereof. It has been found that the PRL-1 protein has a tertiary structure having 5 strands of beta-sheet surrounded by 6 alpha-helices and well-arranged active site with closed P-loop, and monomers form a trimer through farnesylation site in the C-terminus of said protein. Thus intra-cellular migration and membrane localization can be achieved. The said crystal structure of PRL-1 protein of the present invention is very useful for the development the agent which inhibits carcinogenesis and metastasis of the cancer.

    摘要翻译： 本发明涉及PRL-1（再生肝磷酸酶）蛋白的晶体结构及其结晶方法。 已经发现，PRL-1蛋白具有三级结构，其具有由6个α-螺旋包围的5股β片段和具有闭合P环的良好排列的活性位点，并且单体通过C中的法呢基化位点形成三聚体 - 所述蛋白质的末端。 因此，可以实现细胞内迁移和膜定位。 本发明的PRL-1蛋白的所述晶体结构对于抑制癌症的癌发生和转移的药物的开发是非常有用的。

    Structure of prl-1 protein crystal and the method of crystallization thereof
    3.
    发明申请
    Structure of prl-1 protein crystal and the method of crystallization thereof 有权
    prl-1蛋白晶体的结构及其结晶方法

    公开(公告)号：US20090117580A1

    公开(公告)日：2009-05-07

    申请号：US11587404

    申请日：2005-04-26

    申请人： Seong Eon Ryu ,  Dae Gwin Jeong ,  Seung-Jun Kim ,  Jae Hoon Kim ,  Jeong Hee Son

    发明人： Seong Eon Ryu ,  Dae Gwin Jeong ,  Seung-Jun Kim ,  Jae Hoon Kim ,  Jeong Hee Son

    IPC分类号： G01N33/53 ,  C12N9/16 ,  C12N15/63 ,  C12N1/21 ,  C12P21/06

    CPC分类号： C12N9/16 ,  C07K2299/00

    摘要： The present invention relates to a crystal structure of PRL-1 (Phospatase of Regenerating Liver) protein and a method of crystallization thereof. It has been found that the PRL-1 protein has a tertiary structure having 5 strands of beta-sheet surrounded by 6 alpha-helices and well-arranged active site with closed P-loop, and monomers form a trimer through farnesylation site in the C-terminus of said protein. Thus intra-cellular migration and membrane localization can be achieved. The said crystal structure of PRL-1 protein of the present invention is very useful for the development the agent which inhibits carcinogenesis and metastasis of the cancer.

    摘要翻译： 本发明涉及PRL-1（再生肝磷酸酶）蛋白的晶体结构及其结晶方法。 已经发现，PRL-1蛋白具有三级结构，其具有由6个α-螺旋包围的5股β片段和具有闭合P环的良好排列的活性位点，并且单体通过C中的法呢基化位点形成三聚体 - 所述蛋白质的末端。 因此，可以实现细胞内迁移和膜定位。 本发明的PRL-1蛋白的所述晶体结构对于抑制癌症的癌发生和转移的药物的开发是非常有用的。