Method for assaying receptor binding property and reagent for the assay
    3.
    发明授权
    Method for assaying receptor binding property and reagent for the assay 失效
    用于测定受体结合性质的方法和用于测定的试剂

    公开(公告)号:US06638725B2

    公开(公告)日:2003-10-28

    申请号:US09490053

    申请日:2000-01-24

    IPC分类号: G01N3353

    摘要: The present invention provides a method capable of simultaneous processing of plural test samples for the receptor binding property of a chemical substance, which does not require immobilization of the receptor or a special device, and a reagent to be used for this method. That is a method for assaying the receptor binding property of an assay target substance is provided, the method comprising the steps of (a) competitively reacting a known concentration of a ligand and the assay target substance with a known concentration of the receptor in a solution, (b) measuring, without physically removing the ligand bound with the receptor prior to the assay, the amount of a free ligand in the solution using one or more antibodies against the ligand, and (c) determining the receptor binding property of the assay target substance using the amount of the free ligand as an index.

    摘要翻译: 本发明提供一种能够同时处理多个测试样品用于不需要固定受体或特殊装置的化学物质的受体结合特性的试剂和用于该方法的试剂。 这是一种用于测定测定目标物质的受体结合性质的方法,该方法包括以下步骤:(a)将已知浓度的配体和测定目标物质与已知浓度的受体在溶液中竞争性地反应 ,(b)在测定前物理除去与受体结合的配体,测量溶液中使用一种或多种针对配体的抗体的游离配体的量,以及(c)测定该测定的受体结合性质 使用游离配体的量作为指标的目标物质。