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1.发明授权公开(公告)号：US06638725B2
公开(公告)日：2003-10-28
申请号：US09490053
申请日：2000-01-24
IPC分类号： G01N3353
CPC分类号： G01N33/743 , G01N33/53 , G01N33/54306 , G01N2333/723 , Y10S530/812 , Y10S530/816 , Y10S530/827
摘要： The present invention provides a method capable of simultaneous processing of plural test samples for the receptor binding property of a chemical substance, which does not require immobilization of the receptor or a special device, and a reagent to be used for this method. That is a method for assaying the receptor binding property of an assay target substance is provided, the method comprising the steps of (a) competitively reacting a known concentration of a ligand and the assay target substance with a known concentration of the receptor in a solution, (b) measuring, without physically removing the ligand bound with the receptor prior to the assay, the amount of a free ligand in the solution using one or more antibodies against the ligand, and (c) determining the receptor binding property of the assay target substance using the amount of the free ligand as an index.
摘要翻译： 本发明提供一种能够同时处理多个测试样品用于不需要固定受体或特殊装置的化学物质的受体结合特性的试剂和用于该方法的试剂。 这是一种用于测定测定目标物质的受体结合性质的方法，该方法包括以下步骤：（a）将已知浓度的配体和测定目标物质与已知浓度的受体在溶液中竞争性地反应 ，（b）在测定前物理除去与受体结合的配体，测量溶液中使用一种或多种针对配体的抗体的游离配体的量，以及（c）测定该测定的受体结合性质 使用游离配体的量作为指标的目标物质。
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公开(公告)号：US07659060B2
公开(公告)日：2010-02-09
申请号：US10653586
申请日：2003-09-02
CPC分类号： C12Q1/6827 , C12Q2565/101 , C12Q2563/173
摘要： A method for identifying a nucleotide polymorphism, the method comprising hybridizing a labeled oligonucleotide for a wild type or a mutant type to a nucleic acid containing a specific nucleotide polymorphic site in a sample; allowing a nucleic acid-specific label to act thereon; detecting an interaction between the label of the oligonucleotide and the nucleic acid-specific label; and identifying a nucleotide polymorphism, wherein the identification utilizes a difference due to the nucleotide polymorphism in conditions under which the hybridized oligonucleotide is separated into a single strand.
摘要翻译： 一种用于鉴定核苷酸多态性的方法，所述方法包括将野生型或突变型的标记寡核苷酸与含有样品中特定核苷酸多态性位点的核酸杂交; 允许核酸特异性标记作用于其上; 检测寡核苷酸的标记与核酸特异性标记之间的相互作用; 并鉴定核苷酸多态性，其中鉴定在杂交寡核苷酸被分离成单链的条件下利用由于核苷酸多态性引起的差异。
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公开(公告)号：US06455684B1
公开(公告)日：2002-09-24
申请号：US09967361
申请日：2001-09-28
IPC分类号： C07H2102
CPC分类号： G01N33/582 , G01N2458/40
摘要： A method for analyzing an objective substance, comprising reacting a labeled probe with an objective substance on a biological sample, said probe comprising a label substance of the formula (I): wherein A1 is an aromatic group, R1 is a hydrogen or —COCH2COCnF2n+1 and n is an integer of 1-6, which is bonded to a probe selected from the group consisting of nucleic acid, nucleic acid binding protein, low molecular ligand and receptor for ligand (except antibody) to give a fluorescent complex, reacting the complex with an objective substance on a biological sample and assaying fluorescence of the resultant fluorescent complex, a labeled nucleic acid probe and a labeled nucleotide. According to the method of the present invention, defects such as hindrance of fluorescence due to contaminant substance, low sensitivity and the like can be resolved, thereby enabling analysis on a tissue.
摘要翻译： 一种用于分析目标物质的方法，包括使标记的探针与生物样品上的目标物质反应，所述探针包含式（I）的标记物质：其中A1为芳族基团，R1为氢或-COCH2COCnF2n + 1和n是1-6的整数，其与选自核酸，核酸结合蛋白，低分子配体和配体（抗体除外）的受体的探针结合，得到荧光复合物，使 与生物样品上的目标物质复合，并测定所得荧光复合物，标记的核酸探针和标记的核苷酸的荧光。 根据本发明的方法，可以解决诸如由于污染物质引起的荧光阻碍，低灵敏度等的缺陷，从而能够对组织进行分析。
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公开(公告)号：US06339172B1
公开(公告)日：2002-01-15
申请号：US09301406
申请日：1999-04-28
IPC分类号： C07C30900
CPC分类号： G01N33/582 , G01N2458/40
摘要： A method for analyzing an objective substance, comprsing reacting a labeled probe with an objective substance on a biological sample, said probe comprsing a label substance of the formula (I): wherein A1 is an aromatic group, R1 is a hydrogen or —COCH2COCnF2n+1 and n is an integer of 1-6, which is bonded to a probe selected from the group consisting of nucleic acid, nudeic acid binding potein, low molecular ligand and receptor for ligand (except antibody) to give a fluorescent complex, reacting the complex with an objective substance on a biolgical sample and assaying fluorescence of the resultant fluorescent complex, a labeled nucleic acid probe and a labeled nucleotide. According to the method of the present invention, defects such as hindrance of fluorescence due to contaminant substance, low sensitivity and the like can be resolved, thereby enabling analysis on a tissue.
摘要翻译： 一种用于分析目标物质的方法，包括使标记的探针与生物样品上的目标物质反应，所述探针包含式（I）的标记物质：其中A1是芳族基团，R1是氢或-COCH2COCnF2n + 1，n为1-6的整数，其与选自核酸，裸露酸结合蛋白，低分子配体和配体受体（抗体除外）的探针结合，得到荧光复合物，使 与目标物质在生物样品上复合并测定所得荧光复合物的荧光，标记的核酸探针和标记的核苷酸。 根据本发明的方法，可以解决诸如由于污染物质引起的荧光阻碍，低灵敏度等的缺陷，从而能够对组织进行分析。
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公开(公告)号：US08419995B2
公开(公告)日：2013-04-16
申请号：US12563461
申请日：2009-09-21
申请人： Ikuo Yoneda , Kentaro Matsunaga , Yukiko Kikuchi , Yoshihisa Kawamura , Eishi Shiobara , Shinichi Ito , Tetsuro Nakasugi , Hirokazu Kato
发明人： Ikuo Yoneda , Kentaro Matsunaga , Yukiko Kikuchi , Yoshihisa Kawamura , Eishi Shiobara , Shinichi Ito , Tetsuro Nakasugi , Hirokazu Kato
CPC分类号： B29C37/0003 , B29C35/0888 , B29C37/006 , B29C59/022 , B29C2035/0822 , B29C2035/0827 , B29C2059/023 , B82Y10/00 , B82Y40/00 , G03F7/0002
摘要： An imprint method includes applying a light curable resin on a substrate to be processed, the substrate including first and second regions on which the light curable resin is applied, contacting an imprint mold with the light curable resin, curing the light curable resin by irradiating the light curable resin with light passing through the imprint mold, generating gas by performing a predetermined process to the light curable resin applied on a region of the substrate, the region including at least the first region, wherein an amount of gas generated from the light curable resin applied on the first region is larger than an amount of gas generated from the light curable resin of the second region, and forming a pattern by separating the imprint mold from the light curable resin after the gas being generated.
摘要翻译： 压印方法包括将光固化树脂施加在待加工的基板上，所述基板包括施加有光固化树脂的第一和第二区域，使印模与光固化树脂接触，通过照射光固化树脂来固化光固化树脂 光固化树脂，其光通过压印模具，通过对施加在基板的区域上的光固化树脂进行预定处理产生气体，该区域至少包括第一区域，其中从光可固化 施加在第一区域上的树脂大于由第二区域的光固化树脂产生的气体的量，并且在产生气体之后通过从光固化树脂分离压印模具来形成图案。
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6.发明申请公开(公告)号：US20110034029A1
公开(公告)日：2011-02-10
申请号：US12849599
申请日：2010-08-03
IPC分类号： H01L21/306
CPC分类号： H01L21/0337 , B81C1/00031 , B81C2201/0149
摘要： According to one embodiment, a pattern formation method is disclosed. The method includes forming a plurality of regions on a foundation and the plurality of the regions correspond to different pattern sizes. The method includes separating each of a plurality of block copolymers from another one of the plurality of the block copolymers and segregating the each of the plurality of the block copolymers into a corresponding one of the regions. The method includes performing a phase separation of the each of the block copolymers of each of the regions. The method includes selectively removing a designated phase of each of the phase-separated block copolymers to form a pattern of the each of the block copolymers and the pattern has a different pattern size for the each of the regions.
摘要翻译： 根据一个实施例，公开了图案形成方法。 该方法包括在基础上形成多个区域，并且多个区域对应于不同的图案尺寸。 该方法包括从多个嵌段共聚物中的另一个嵌段共聚物中分离多个嵌段共聚物，并将多个嵌段共聚物中的每一个分离成相应的一个区域。 该方法包括进行每个区域的每个嵌段共聚物的相分离。 该方法包括选择性地除去每个相分离的嵌段共聚物的指定相以形成每个嵌段共聚物的图案，并且该图案对于每个区域具有不同的图案尺寸。
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公开(公告)号：US07422882B2
公开(公告)日：2008-09-09
申请号：US09852922
申请日：2001-05-10
申请人： Toshihiro Kuroita , Masao Kitabayashi , Yoshikazu Ishida , Hideyuki Komatsubara , Yoshiaki Nishiya , Bunsei Kawakami , Yoshihisa Kawamura , Tadayuki Imanaka
发明人： Toshihiro Kuroita , Masao Kitabayashi , Yoshikazu Ishida , Hideyuki Komatsubara , Yoshiaki Nishiya , Bunsei Kawakami , Yoshihisa Kawamura , Tadayuki Imanaka
CPC分类号： C12N9/1252
摘要： An object of the present invention is to provide a thermostable DNA polymerase with enhanced amplification efficiency and/or improved fidelity in polymerase chain reaction (PCR), and provide a process for production thereof. More specifically, the present invention provides thermostable DNA polymerase wherein in the DX1EX2X3X4H sequence (D: aspartic acid, E: glutamic acid, H: histidine, X1, X2, X3 and X4: any amino acid) consisting of DX1E sequence within the EXO I region and a four amino acid length peptide adjacent to said glutamic acid(E) of thermostable DNA polymerase having 3′-5′ exonuclease activity, histidine(H) has been replaced by another amino acid.
摘要翻译： 本发明的目的是提供具有增强的扩增效率和/或聚合酶链反应（PCR）中的改进的保真度的热稳定性DNA聚合酶，并提供其生产方法。 更具体地说，本发明提供了热稳定的DNA聚合酶，其中在X 1，X 2，X 3，X 4， 序列（D：天冬氨酸，E：谷氨酸，H：组氨酸，X 1，X 2，X 3和X 3， 由SEQ ID NO：1区域内的DX1E序列组成的4个氨基酸长度肽与邻近所述谷氨酸（E）的4个氨基酸长度的肽组成，所述热稳定性DNA聚合酶具有3' -5'核酸外切酶活性，组氨酸（H）已被另一种氨基酸取代。
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8.发明授权Compositions for enhancing DNA synthesis, DNA polymerase-related factors and utilization thereof 有权用于增强DNA合成，DNA聚合酶相关因子及其利用的组合物公开(公告)号：US07384739B2
公开(公告)日：2008-06-10
申请号：US10495581
申请日：2002-11-14
申请人： Masao Kitabayashi , Toshihiro Kuroita , Yoshikazu Ishida , Hideyuki Komatsubara , Yoshiaki Nishiya , Masanori Oka , Yoshihisa Kawamura , Tadayuki Imanaka
发明人： Masao Kitabayashi , Toshihiro Kuroita , Yoshikazu Ishida , Hideyuki Komatsubara , Yoshiaki Nishiya , Masanori Oka , Yoshihisa Kawamura , Tadayuki Imanaka
CPC分类号： C07K14/195 , C12N9/1252 , C12Q1/6846 , C12Q2527/125 , C12Q2521/101
摘要： The invention provides methods, kits, and compositions for enhancing synthesis of DNA involving a carboxylate ion-supplying substance that is effective in promoting DNA synthesis in enzymatic DNA synthesis reactions. The invention further provides a thermostable DNA polymerase-related factor derived from Thermococcus species, which has an activity to promote the DNA synthesis activity of DNA polymerase or which binds to DNA polymerase.
摘要翻译： 本发明提供用于增强DNA合成的方法，试剂盒和组合物，所述方法，试剂盒和组合物涉及在酶促DNA合成反应中有效促进DNA合成的羧酸根离子供应物质。 本发明还提供了源自Thermococcus种的热稳定DNA聚合酶相关因子，其具有促进DNA聚合酶的DNA合成活性或与DNA聚合酶结合的活性。
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公开(公告)号：US06387646B1
公开(公告)日：2002-05-14
申请号：US09458147
申请日：1999-12-09
IPC分类号： C12Q140
CPC分类号： G01N33/84 , C12Q1/40 , C12Q2334/00 , C12Q2334/10 , G01N2800/04 , Y10S435/963
摘要： The present invention provides a combination of reagent compositions for measuring an electrolyte which are excellent in stability, precision and quantitativity and have high solution stability sufficient to withstand distribution. In the combination of the reagent compositions of the present invention, a chelating agent and an inactivated &agr;-amylase capable of being reversibly activated by the electrolyte are formulated separately from each other.
摘要翻译： 本发明提供了稳定性，精度和定量优异且具有足以抵抗分布的高溶液稳定性的用于测量电解质的试剂组合物的组合。 在本发明的试剂组合物的组合中，能够由电解质可逆地活化的螯合剂和灭活的α-淀粉酶彼此分开配制。
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公开(公告)号：US06350585B1
公开(公告)日：2002-02-26
申请号：US09253020
申请日：1999-02-19
IPC分类号： C12Q140
CPC分类号： G01N33/573 , C12Q1/40 , C12Q2334/00 , C12Q2334/10
摘要： A reagent for amylase isozyme activity assay, which contains 2-chloro-4-nitrophenyl 4-O-&bgr;-D-galactopyranosylmaltoside, an antibody inhibiting S-type amylase activity or P-type amylase activity, and an amylase activator, and methods for assaying amylase isozyme activity using the regent is provided. According to the present invention, amylase isozyme activity can be accurately and easily assayed using a low concentration activator and an antibody having S-type amylase or P-type amylase activity inhibitory action without using an adjuvant enzyme.
摘要翻译： 含有2-氯-4-硝基苯基4-O-β-D-吡喃半乳糖基麦芽糖苷，抑制S型淀粉酶活性或P型淀粉酶活性的抗体和淀粉酶活化剂的淀粉酶同工酶活性测定试剂和方法 提供使用摄取剂测定淀粉酶同工酶活性。 根据本发明，在不使用佐剂酶的情况下，可以使用低浓度活化剂和具有S型淀粉酶或P型淀粉酶活性抑制作用的抗体，能够准确且容易地测定淀粉酶同功酶活性。
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