公开(公告)号：US5861124A

公开(公告)日：1999-01-19

申请号：US296893

申请日：1994-08-29

申请人： Shigeru Hosoi ,  Tadashi Fukami ,  Akihiko Tsuji ,  Makiko Hiyoshi

发明人： Shigeru Hosoi ,  Tadashi Fukami ,  Akihiko Tsuji ,  Makiko Hiyoshi

IPC分类号： C12Q1/68 ,  G01N21/64

CPC分类号： G01N21/6428 ,  C12Q1/68

摘要： An apparatus for detecting denaturation of a nucleic acid, including: denaturation condition controlling means for controlling condition of denaturation under which a double-stranded nucleic acid is separated into a first single-stranded nucleic acid and a second single-stranded nucleic acid; excitation light irradiation means for irradiating the double-stranded nucleic acid before denaturation, and the first single-stranded nucleic acid and second single-stranded nucleic acid after the denaturation; fluorescence detection means for detecting fluorescence emission based on the excitation light irradiation; and processing means for receiving, storing and processing a signal supplied from the fluorescence detection means.

摘要翻译： 一种用于检测核酸变性的装置，包括：变性条件控制装置，用于控制双链核酸分离成第一单链核酸和第二单链核酸的变性条件; 用于在变性前照射双链核酸的激发光照射装置，以及变性后的第一单链核酸和第二单链核酸; 用于根据激发光照射检测荧光发射的荧光检测装置; 以及用于接收，存储和处理从荧光检测装置提供的信号的处理装置。

公开(公告)号：US5599504A

公开(公告)日：1997-02-04

申请号：US275910

申请日：1994-07-15

申请人： Shigeru Hosoi ,  Tadashi Fukami ,  Akihiko Tsuji

发明人： Shigeru Hosoi ,  Tadashi Fukami ,  Akihiko Tsuji

IPC分类号： C12N15/00 ,  C12Q1/68 ,  G01N21/64

CPC分类号： G01N21/6428 ,  C12Q1/68

摘要： An apparatus for detecting denaturation of a nucleic acid, including: denaturation condition controlling element for controlling condition of denaturation under which a double-strand nucleic acid is separated into a first single-strand nucleic acid and a second single-strand nucleic acid; excitation light irradiation source for irradiating the double-strand nucleic acid before denaturation, and the first single-strand nucleic acid and second single-strand nucleic acid after the denaturation; fluorescence detection monitor for detecting fluorescence emission based on the excitation light irradiation; and processing element for receiving, storing and processing a signal supplied from the fluorescence detection monitor.

摘要翻译： 一种用于检测核酸变性的装置，包括：变性条件控制元件，用于控制双链核酸分离成第一单链核酸和第二单链核酸的变性条件; 用于在变性前照射双链核酸的激发光照射源和变性后的第一单链核酸和第二单链核酸; 用于基于激发光照射检测荧光发射的荧光检测监视器; 以及用于接收，存储和处理从荧光检测监视器提供的信号的处理元件。

公开(公告)号：US5260029A

公开(公告)日：1993-11-09

申请号：US917384

申请日：1992-07-23

申请人： Shigeru Hosoi ,  Tsuyoshi Hayakawa

发明人： Shigeru Hosoi ,  Tsuyoshi Hayakawa

IPC分类号： C12Q1/68 ,  G01J1/02 ,  G01N15/14 ,  G01N21/64 ,  G01N33/50 ,  H01J43/04

CPC分类号： G01N21/6486 ,  G01N15/1459 ,  H01J43/04

摘要： To assure detection of fluorescence produced from continuously supplied fine particles such as DNA bases with accuracy and excellent efficiency, fine particles supplied from a flow cell are introduced into a through-hole of a hollow photomultiplier. An optical fiber is disposed in axial alignment with the through-hole of the photomultiplier and irradiates an exciting light beam onto the fine particles staying in the through-hole. Light-induced fluorescence is thus detected by the photomultiplier which has a photocathode on an inner circumference of the through-hole.

公开(公告)号：US06255048B1

公开(公告)日：2001-07-03

申请号：US09011223

申请日：1998-02-09

申请人： Shigeru Hosoi ,  Makiko Kojima ,  Sachiko Kadouchi

发明人： Shigeru Hosoi ,  Makiko Kojima ,  Sachiko Kadouchi

IPC分类号： C12Q168

CPC分类号： G01N33/582 ,  C12Q1/6834 ,  G01N33/533 ,  Y10S435/968 ,  Y10S436/828 ,  C12Q2563/107 ,  C12Q2545/114 ,  C12Q2565/601

摘要： This invention provides fluoroassay which comprises labeling analyte molecules with a fluorescent material having a nucleic acid portion stained with a sufficient number of fluorochrome molecules so as to be measurable as fluorescent spots, and a reactive group binding to the analyte molecule specifically, immobilizing the labeled analyte on a solid phase, and counting the number of fluorescent spots. The nucleic acid portion of the fluorescent labeling material is a double-stranded or single-stranded nucleic acid, and the staining with the fluorochrome molecules is performed with intercalating type, minor groove binding type, or covalently binding to the nucleic acid type.

摘要翻译： 本发明提供了荧光测定法，其包括用荧光材料标记分析物分子，该荧光材料具有用足够数量的荧光染料分子染色的核酸部分，以便可测量为荧光斑点，以及特异性结合分析物分子的反应性基团，将标记的分析物 在固相上，并计数荧光斑数。 荧光标记材料的核酸部分是双链或单链核酸，并且用插入型，小沟结合型或共价结合核酸型进行用荧光染料分子的染色。

公开(公告)号：US06952670B2

公开(公告)日：2005-10-04

申请号：US09907394

申请日：2001-07-17

申请人： Shogo Iizuka ,  Shigeru Hosoi ,  Kazuki Hoshino

发明人： Shogo Iizuka ,  Shigeru Hosoi ,  Kazuki Hoshino

IPC分类号： G10L11/02 ,  G10L15/02 ,  G10L15/04 ,  G10L19/00

CPC分类号： G10L25/78

摘要： An extraction section extracts a speech signal having ambient noise superimposed thereon as a data segment having a predetermined duration. An autocorrelation function normalizing section determines normalized autocorrelation function vectors. A normalized autocorrelation function count section counts a given number of normalized autocorrelation function vectors. A noise vector region/speech vector region/undefined vector computation section classifies the normalized autocorrelation function vectors into any of a noise vector region, a speech vector region, or undefined vectors. When the latest normalized autocorrelation function vector acquired by a normalized autocorrelation function vector determination section pertains to the noise vector region, the speech signal is determined to be a noise segment. In contrast, when the latest vector does not pertain to the noise vector region, the input signal is determined to be a speech segment.

摘要翻译： 提取部分提取具有叠加在其上的具有环境噪声的语音信号作为具有预定持续时间的数据段。 自相关函数归一化部分确定归一化的自相关函数向量。 归一化自相关函数计数部分对给定数量的归一化自相关函数向量进行计数。 噪声矢量区域/语音矢量区域/未定义矢量计算部分将归一化的自相关函数矢量分类为噪声矢量区域，语音矢量区域或未定义矢量中的任何一个。 当由归一化自相关函数向量确定部分获取的最新归一化自相关函数向量与噪声矢量区域相关时，语音信号被确定为噪声段。 相反，当最新矢量不属于噪声矢量区域时，输入信号被确定为语音段。

公开(公告)号：US06376184B1

公开(公告)日：2002-04-23

申请号：US09537720

申请日：2000-03-30

申请人： Shigeru Hosoi ,  Michiko Furuki

发明人： Shigeru Hosoi ,  Michiko Furuki

IPC分类号： C12Q168

CPC分类号： C12Q1/6874 ,  C12Q2565/537 ,  C12Q2565/125

摘要： The base sequence data of a gene is analyzed. A set of degenerate probes are hybridized to a single-stranded nucleic acid analyte derived from a gene, the nucleic acid analyte is used as a template and the probes are used as primers for a thermo-cycled polymerase reaction, the reaction products obtained from the respective probes are separated by gel electrophoresis and the electrophoresis patterns for the probes are compared, to allow for feature extraction of the base sequence of the nucleic acid analyte, including its sequencing.

摘要翻译： 分析基因的碱基序列数据。 一组简并探针与衍生自基因的单链核酸分析物杂交，将核酸分析物用作模板，并将探针用作热循环聚合酶反应的引物，从 通过凝胶电泳分离各个探针，并比较探针的电泳图案，以允许核酸分析物的碱基序列的特征提取，包括其测序。