公开(公告)号：US20190194286A1

公开(公告)日：2019-06-27

申请号：US16327193

申请日：2016-07-29

申请人： Shigeyuki YOKOYAMA

发明人： Shigeyuki YOKOYAMA ,  Takehiro SHINODA ,  Kaori ITO ,  Naoko NOMURA ,  Yoshiko KATSURA ,  Tomomi SOMEYA ,  Mikako SHIROUZU ,  Tetsuya HORI ,  Yoshihiro NAKAMURA ,  Hiroaki TANABE

IPC分类号： C07K14/705 ,  C12N9/64

CPC分类号： C07K14/705 ,  C12N9/6478 ,  C12N15/09 ,  C12P21/02 ,  C12Y304/23046

摘要： In order to provide a membrane protein production method which does not require the step of solubilizing a membrane protein and which allows the membrane protein having an excellent quality to be obtained with a high yield, a method in accordance with an embodiment of the present invention includes: a step (a) of preparing a reaction solution for cell-free protein synthesis, the reaction solution containing (i) a template nucleic acid which encodes the membrane protein, (ii) a lipid, and (iii) a detergent which is contained at a concentration equal to or higher than a critical micelle concentration; and a step (b) of synthesizing the membrane protein while the concentration of the detergent in the reaction solution is maintained at a concentration equal to or higher than a critical micelle concentration.