公开(公告)号：US07303890B2

公开(公告)日：2007-12-04

申请号：US10381710

申请日：2001-09-27

申请人： Shiken Sha ,  Hidehito Mukai ,  Yoshiko Aoki ,  Yoshisuke Nishi

发明人： Shiken Sha ,  Hidehito Mukai ,  Yoshiko Aoki ,  Yoshisuke Nishi

IPC分类号： G01N33/567

CPC分类号： C07K14/47 ,  A61K38/00 ,  A61K48/00 ,  C07K14/535 ,  C07K16/18 ,  C07K16/243 ,  C07K2317/34

摘要： The present invention provides, as a gene encoding an antigen recognized by a G-CSF inducing antibody, a gene encoding any one of the following proteins: (a) a protein having an amino acid sequence shown in SEQ ID NO; 2; (b) a protein having an amino acid sequence comprising a deletion, substitution, addition or insertion of one or several amino acids with respect to the amino acid sequence shown in SEQ ID NO: 2, and having a binding ability for an antibody which induces and secretes a granulocyte colony-stimulating factor or a fragment thereof: or (c) a protein having at least 50% or more homology with the amino acid sequence shown in SEQ ID NO: 2, and having a binding ability for an antibody capable of inducing and secreting a granulocyte colony-stimulating factor or a fragment thereof.

摘要翻译： 作为编码由G-CSF诱导型抗体识别的抗原的基因，本发明提供编码以下蛋白质之一的基因：（a）具有SEQ ID NO所示的氨基酸序列的蛋白质， 2; （b）具有氨基酸序列的氨基酸序列的蛋白质，所述氨基酸序列包含相对于SEQ ID NO：2所示的氨基酸序列的一个或多个氨基酸的缺失，取代，添加或插入，并具有诱导的抗体的结合能力 并分泌粒细胞集落刺激因子或其片段;或（c）与SEQ ID NO：2所示的氨基酸序列具有至少50％以上同源性的蛋白质，并且具有与能够 诱导和分泌粒细胞集落刺激因子或其片段。

公开(公告)号：US07285389B1

公开(公告)日：2007-10-23

申请号：US09937905

申请日：2000-03-31

申请人： Shiken Sha ,  Yoshiko Aoki ,  Yoshisuke Nishi

发明人： Shiken Sha ,  Yoshiko Aoki ,  Yoshisuke Nishi

IPC分类号： G01N33/50 ,  C12N15/12 ,  C12N15/63 ,  C12N5/10 ,  C07K14/705

CPC分类号： C07K14/47 ,  A61K2039/505 ,  C07K14/535 ,  C07K14/705 ,  C07K16/28 ,  Y10T436/143333

摘要： The present invention provides, as a gene encoding an antigen recognized by G-CSF-inducing antibodies, a gene encoding: (a) a protein having the amino acid sequence listed as SEQ ID NO:2 of the Sequence Listing; (b) a protein having the amino acid sequence listed as SEQ ID NO:2 of the Sequence Listing with one or more amino acid deletions, substitutions, additions or insertions and also binding to an antibody or its fragment that is active to induce granulocyte colony-stimulating factor; or (c) a protein having at least 50% homology with the amino acid sequence listed as SEQ ID NO:2 and also binding to an antibody or its fragment that is active to induce granulocyte colony-stimulating factor.

摘要翻译： 作为编码由G-CSF诱导型抗体识别的抗原的基因，本发明提供编码：（a）具有序列表SEQ ID NO：2所示的氨基酸序列的蛋白质的基因。 （b）具有序列表SEQ ID NO：2所列氨基酸序列的蛋白质，其具有一个或多个氨基酸缺失，取代，添加或插入，并且还结合活性以诱导粒细胞集落的抗体或其片段 刺激因子 或（c）与SEQ ID NO：2所示的氨基酸序列具有至少50％同源性的蛋白质，并且还结合有活性的诱导粒细胞集落刺激因子的抗体或其片段。

公开(公告)号：US20080050386A1

公开(公告)日：2008-02-28

申请号：US11898715

申请日：2007-09-14

申请人： Shiken Sha ,  Yoshiko Aoki ,  Yoshisuke Nishi

发明人： Shiken Sha ,  Yoshiko Aoki ,  Yoshisuke Nishi

IPC分类号： A61K31/7088 ,  A61K38/16 ,  A61K39/395 ,  A61P7/00 ,  C07H21/04 ,  C07K14/00 ,  C07K16/00 ,  G01N33/00

CPC分类号： C07K14/47 ,  A61K2039/505 ,  C07K14/535 ,  C07K14/705 ,  C07K16/28 ,  Y10T436/143333

摘要： The present invention provides, as a gene encoding an antigen recognized by G-CSF-inducing antibodies, a gene encoding: (a) a protein having the amino acid sequence listed as SEQ ID NO:2 of the Sequence Listing; (b) a protein having the amino acid sequence listed as SEQ ID NO:2 of the Sequence Listing with one or more amino acid deletions, substitutions, additions or insertions and also binding to an antibody or its fragment that is active to induce granulocyte colony-stimulating factor; or (c) a protein having at least 50% homology with the amino acid sequence listed as SEQ ID NO:2 and also binding to an antibody or its fragment that is active to induce granulocyte colony-stimulating factor.

摘要翻译： 作为编码由G-CSF诱导型抗体识别的抗原的基因，本发明提供编码：（a）具有序列表SEQ ID NO：2所示的氨基酸序列的蛋白质的基因。 （b）具有序列表SEQ ID NO：2所列氨基酸序列的蛋白质，其具有一个或多个氨基酸缺失，取代，添加或插入，并且还结合活性以诱导粒细胞集落的抗体或其片段 刺激因子 或（c）与SEQ ID NO：2所示的氨基酸序列具有至少50％同源性的蛋白质，并且还结合有活性的诱导粒细胞集落刺激因子的抗体或其片段。

公开(公告)号：US20100316592A1

公开(公告)日：2010-12-16

申请号：US12502959

申请日：2009-07-14

申请人： Teruo AKUTA ,  Yoshiko Aoki

发明人： Teruo AKUTA ,  Yoshiko Aoki

IPC分类号： A61K38/16

CPC分类号： A61K38/195 ,  A61K9/0019 ,  A61K9/08 ,  A61K47/02 ,  A61K47/183

摘要： An objective of the present invention is to provide aqueous preparations comprising eMIP, a derivative of macrophage inflammatory protein 1α (MIP-1α) which has an immunopotentiation activity, and stabilizer(s). The present inventors conducted dedicated studies to achieve the above-described objective. As a result, the present inventors discovered that eMIP degradation is suppressed by addition of at least one or more additives selected from sodium chloride, L-histidine, L-arginine, L-arginine hydrochloride, L-lysine hydrochloride, citric acid, and sodium edetate (EDTA). Furthermore, the present inventors demonstrated that phosphate buffer of pH 5 to pH 7 is a preferable pH adjuster for the aqueous eMIP preparations of the present invention.

摘要翻译： 本发明的目的是提供包含eMIP，具有免疫增强活性的巨噬细胞炎症蛋白1α（MIP-1α）的衍生物和稳定剂的含水制剂。 本发明人进行了专门的研究以实现上述目的。 结果，本发明人发现通过添加选自氯化钠，L-组氨酸，L-精氨酸，L-精氨酸盐酸盐，L-赖氨酸盐酸盐，柠檬酸和钠中的至少一种或多种添加剂，可以抑制eMIP降解 乙二胺四乙酸（EDTA）。 此外，本发明人证明，pH5〜pH7的磷酸盐缓冲液是本发明的含水eMIP制剂的优选的pH调节剂。