公开(公告)号：US20090187011A1

公开(公告)日：2009-07-23

申请号：US12308936

申请日：2007-06-29

申请人： Shin-Ichiro Nishimura ,  Yasuro Shinohara ,  Yoshiaki Miura ,  Jun-ichi Furukawa ,  Yoko Kita ,  Akio Takimoto ,  Mika Nakano

发明人： Shin-Ichiro Nishimura ,  Yasuro Shinohara ,  Yoshiaki Miura ,  Jun-ichi Furukawa ,  Yoko Kita ,  Akio Takimoto ,  Mika Nakano

IPC分类号： C07K1/12 ,  C07C233/02

CPC分类号： C07C309/15 ,  C12P19/00 ,  G01N33/66

摘要： A means for pre-treatment in glycomic analysis of a glycoprotein is provided by the present invention.A salt of the general formula (I): wherein Z, X, R1, R2, M, m and n are the same as described in DESCRIPTION, is useful as a protein solubilizer, and an oligosaccharide is efficiently released from a sample if reductive alkylation and/or digestion by a proteinase are carried out under the presence of the said solubilizer at the first step in the glycomic analysis of glycoprotein derived from a living body.

摘要翻译： 本发明提供了糖蛋白糖基分析中预处理的手段。 通式（I）的盐：其中Z，X，R 1，R 2，M，m和n与描述中所述相同，可用作蛋白质增溶剂，并且如果还原，寡糖有效地从样品中释放 通过蛋白酶的烷基化和/或消化在所述增溶剂的存在下在来自活体的糖蛋白的糖基分析中的第一步进行。

公开(公告)号：US08058409B2

公开(公告)日：2011-11-15

申请号：US12308936

申请日：2007-06-29

申请人： Shin-Ichiro Nishimura ,  Yasuro Shinohara ,  Yoshiaki Miura ,  Jun-ichi Furukawa ,  Yoko Kita ,  Akio Takimoto ,  Mika Nakano

发明人： Shin-Ichiro Nishimura ,  Yasuro Shinohara ,  Yoshiaki Miura ,  Jun-ichi Furukawa ,  Yoko Kita ,  Akio Takimoto ,  Mika Nakano

IPC分类号： C08H1/00 ,  C07C233/00 ,  C07C309/62 ,  C12Q1/37

CPC分类号： C07C309/15 ,  C12P19/00 ,  G01N33/66

摘要： A means for pre-treatment in glycomic analysis of a glycoprotein is provided by the present invention.A salt of the general formula (I): wherein Z, X, R1, R2, M, m and n are the same as described in DESCRIPTION, is useful as a protein solubilizer, and an oligosaccharide is efficiently released from a sample if reductive alkylation and/or digestion by a proteinase are carried out under the presence of the said solubilizer at the first step in the glycomic analysis of glycoprotein derived from a living body.

摘要翻译： 本发明提供了糖蛋白糖基分析中预处理的手段。 通式（I）的盐：其中Z，X，R 1，R 2，M，m和n与描述中所述相同，可用作蛋白质增溶剂，并且如果还原，寡糖有效地从样品中释放 通过蛋白酶的烷基化和/或消化在所述增溶剂的存在下在来自活体的糖蛋白的糖基分析中的第一步进行。

公开(公告)号：US08877454B2

公开(公告)日：2014-11-04

申请号：US12681573

申请日：2008-10-03

申请人： Shinichiro Nishimura ,  Yasuro Shinohara ,  Yoshiaki Miura ,  Hiroshi Yamazaki ,  Michio Horiuchi ,  Hiroaki Motoki ,  Toshiharu Kuroda ,  Yoko Kita ,  Mika Nakano

发明人： Shinichiro Nishimura ,  Yasuro Shinohara ,  Yoshiaki Miura ,  Hiroshi Yamazaki ,  Michio Horiuchi ,  Hiroaki Motoki ,  Toshiharu Kuroda ,  Yoko Kita ,  Mika Nakano

IPC分类号： C12Q1/37 ,  G01N33/68 ,  G01N30/72 ,  H01J49/00

CPC分类号： G01N33/6848 ,  G01N30/7233 ,  G01N2400/00 ,  H01J49/00

摘要： To provide an autoanalyzer for analyzing a sugar chain contained in a biological sample, in particular, serum. Namely, it is intended to provide a method of analyzing a sugar chain in a sample, which comprises the following steps: A) the sugar chain-releasing step of releasing the sugar chain in the sample; B) the detection sample-preparing step of preparing the released sugar chain for detection; and, in the case of conducting mass spectrometry using a plate, C) the step of forming a plate for the mass spectrometry having the captured sugar chain dotted thereon which comprises the step of providing the tagged sugar chain sample solution obtained in the step B) on a collection plate; and, if required, the step of conducting an operation in a solid phase support-enclosed plate to form the plate for mass spectrometry; and D) the step of analyzing the sugar chain to be assayed.

摘要翻译： 提供一种用于分析生物样品，特别是血清中所含的糖链的自动分析仪。 即，提供一种分析样品中的糖链的方法，其包括以下步骤：A）释放样品中的糖链的糖链释放步骤; B）制备释放的糖链进行检测的检测样品制备步骤; 并且在使用板进行质谱分析的情况下，C）形成具有点状捕获的糖链的用于质谱的板的步骤，其包括提供步骤B）中获得的标记的糖链样品溶液的步骤， 在收集板上; 如果需要，在固相支持封闭板中进行操作以形成质谱板的步骤; 和D）分析要测定的糖链的步骤。

公开(公告)号：US20110111443A1

公开(公告)日：2011-05-12

申请号：US12681573

申请日：2008-10-03

申请人： Shinichiro Nishimura ,  Yasuro Shinohara ,  Yoshiaki Miura ,  Hiroshi Yamazaki ,  Michio Horiuchi ,  Hiroaki Motoki ,  Toshiharu Kuroda ,  Yoko Kita ,  Mika Nakano

发明人： Shinichiro Nishimura ,  Yasuro Shinohara ,  Yoshiaki Miura ,  Hiroshi Yamazaki ,  Michio Horiuchi ,  Hiroaki Motoki ,  Toshiharu Kuroda ,  Yoko Kita ,  Mika Nakano

IPC分类号： C12Q1/37 ,  C12M1/34 ,  C12P19/00 ,  C40B60/14

CPC分类号： G01N33/6848 ,  G01N30/7233 ,  G01N2400/00 ,  H01J49/00

摘要： To provide an autoanalyzer for analyzing a sugar chain contained in a biological sample, in particular, serum. Namely, it is intended to provide a method of analyzing a sugar chain in a sample, which comprises the following steps: A) the sugar chain-releasing step of releasing the sugar chain in the sample; B) the detection sample-preparing step of preparing the released sugar chain for detection; and, in the case of conducting mass spectrometry using a plate, C) the step of forming a plate for the mass spectrometry having the captured sugar chain dotted thereon which comprises the step of providing the tagged sugar chain sample solution obtained in the step B) on a collection plate; and, if required, the step of conducting an operation in a solid phase support-enclosed plate to form the plate for mass spectrometry; and D) the step of analyzing the sugar chain to be assayed.

摘要翻译： 提供一种用于分析生物样品，特别是血清中所含的糖链的自动分析仪。 即，提供一种分析样品中的糖链的方法，其包括以下步骤：A）释放样品中的糖链的糖链释放步骤; B）制备释放的糖链进行检测的检测样品制备步骤; 并且在使用板进行质谱分析的情况下，C）形成具有点状捕获的糖链的用于质谱的板的步骤，其包括提供步骤B）中获得的标记的糖链样品溶液的步骤， 在收集板上; 如果需要，在固相支持封闭板中进行操作以形成质谱板的步骤; 和D）分析要测定的糖链的步骤。